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Methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis B
The stimulator of interferon genes (STING) plays a crucial role in the recognition of a viral infection and subsequent stimulation of an immune response. However, it is unclear whether methylation of the STING promoter affects STING transcription and response to antiviral therapy. The present study...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314766/ https://www.ncbi.nlm.nih.gov/pubmed/30593207 http://dx.doi.org/10.1097/MD.0000000000013904 |
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author | Wu, Chen-Si Zhao, Qian Zhang, Jun Wang, Jing-Wen Qian, Yu Fan, Yu-Chen Wang, Kai |
author_facet | Wu, Chen-Si Zhao, Qian Zhang, Jun Wang, Jing-Wen Qian, Yu Fan, Yu-Chen Wang, Kai |
author_sort | Wu, Chen-Si |
collection | PubMed |
description | The stimulator of interferon genes (STING) plays a crucial role in the recognition of a viral infection and subsequent stimulation of an immune response. However, it is unclear whether methylation of the STING promoter affects STING transcription and response to antiviral therapy. The present study determined the methylation status of the STING promoter in patients with chronic hepatitis B (CHB). This study included 198 participants, of which 159 participants had CHB and 39 were healthy controls (HCs). Methylation-specific polymerase chain reaction was performed to detect the methylation status of the STING promoter. Reverse transcription-quantitative polymerase chain reaction was performed to determine STING mRNA level in peripheral blood mononuclear cells. The methylation frequency of the STING promoter was significantly higher and STING mRNA level was lower in the patients with CHB than in the HCs. Presence of hepatitis B virus (HBV) DNA was independently correlated with an increased risk of STING promoter methylation. Virological response frequency was higher in the patients with CHB receiving entecavir (ETV) than in those receiving adefovir (ADV). In the ETV group, the virological response frequency was evidently lower in the patients with CHB having methylated STING promoters than in those having unmethylated STING promoters. However, there was no significant difference in the virological response frequency between ADV-treated patients having methylated and unmethylated STING promoters. These results indicate that the hypermethylation of the STING promoter and thus the transcriptional repression of STING weaken the effect of STING in inhibiting HBV replication and decreases the effectiveness of antiviral therapy. |
format | Online Article Text |
id | pubmed-6314766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-63147662019-01-14 Methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis B Wu, Chen-Si Zhao, Qian Zhang, Jun Wang, Jing-Wen Qian, Yu Fan, Yu-Chen Wang, Kai Medicine (Baltimore) Research Article The stimulator of interferon genes (STING) plays a crucial role in the recognition of a viral infection and subsequent stimulation of an immune response. However, it is unclear whether methylation of the STING promoter affects STING transcription and response to antiviral therapy. The present study determined the methylation status of the STING promoter in patients with chronic hepatitis B (CHB). This study included 198 participants, of which 159 participants had CHB and 39 were healthy controls (HCs). Methylation-specific polymerase chain reaction was performed to detect the methylation status of the STING promoter. Reverse transcription-quantitative polymerase chain reaction was performed to determine STING mRNA level in peripheral blood mononuclear cells. The methylation frequency of the STING promoter was significantly higher and STING mRNA level was lower in the patients with CHB than in the HCs. Presence of hepatitis B virus (HBV) DNA was independently correlated with an increased risk of STING promoter methylation. Virological response frequency was higher in the patients with CHB receiving entecavir (ETV) than in those receiving adefovir (ADV). In the ETV group, the virological response frequency was evidently lower in the patients with CHB having methylated STING promoters than in those having unmethylated STING promoters. However, there was no significant difference in the virological response frequency between ADV-treated patients having methylated and unmethylated STING promoters. These results indicate that the hypermethylation of the STING promoter and thus the transcriptional repression of STING weaken the effect of STING in inhibiting HBV replication and decreases the effectiveness of antiviral therapy. Wolters Kluwer Health 2018-12-28 /pmc/articles/PMC6314766/ /pubmed/30593207 http://dx.doi.org/10.1097/MD.0000000000013904 Text en Copyright © 2018 the Author(s). Published by Wolters Kluwer Health, Inc. http://creativecommons.org/licenses/by-nc/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial License 4.0 (CCBY-NC), where it is permissible to download, share, remix, transform, and buildup the work provided it is properly cited. The work cannot be used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc/4.0 |
spellingShingle | Research Article Wu, Chen-Si Zhao, Qian Zhang, Jun Wang, Jing-Wen Qian, Yu Fan, Yu-Chen Wang, Kai Methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis B |
title | Methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis B |
title_full | Methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis B |
title_fullStr | Methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis B |
title_full_unstemmed | Methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis B |
title_short | Methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis B |
title_sort | methylation status of the stimulator of interferon genes promoter in patients with chronic hepatitis b |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314766/ https://www.ncbi.nlm.nih.gov/pubmed/30593207 http://dx.doi.org/10.1097/MD.0000000000013904 |
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