Cargando…
A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains
Immunomagnetic separation has been widely used for the separation and concentration of foodborne pathogens from complex food samples, however it can only handle a small volume of samples. In this paper, we presented a novel fluidic device for the specific and efficient separation and concentration o...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315333/ https://www.ncbi.nlm.nih.gov/pubmed/30486364 http://dx.doi.org/10.3390/mi9120624 |
_version_ | 1783384267119656960 |
---|---|
author | Cai, Gaozhe Wang, Siyuan Zheng, Lingyan Lin, Jianhan |
author_facet | Cai, Gaozhe Wang, Siyuan Zheng, Lingyan Lin, Jianhan |
author_sort | Cai, Gaozhe |
collection | PubMed |
description | Immunomagnetic separation has been widely used for the separation and concentration of foodborne pathogens from complex food samples, however it can only handle a small volume of samples. In this paper, we presented a novel fluidic device for the specific and efficient separation and concentration of salmonella typhimurium using self-assembled magnetic nanoparticle chains. The laminated sawtooth-shaped iron foils were first mounted in the 3D-printed matrix and magnetized by a strong magnet to generate dot-array high gradient magnetic fields in the fluidic channel, which was simulated using COMSOL (5.3a, Burlington, MA, USA). Then, magnetic nanoparticles with a diameter of 150 nm, which were modified with the anti-salmonella polyclonal antibodies, were injected into the channel, and the magnetic nanoparticle chains were vertically formed at the dots and verified using a fluorescence inverted microscope. Finally, the bacterial sample was continuous-flow injected, and the target bacteria could be captured by the antibodies on the chains, followed by gold standard culture plating to determine the amount of the target bacteria. Under the optimal conditions, the target bacteria could be separated with a separation efficiency of 80% in 45 min. This fluidic device could be further improved using thinner sawtooth-shaped iron foils and stronger magnets to obtain a better dot-array magnetic field with larger magnetic intensity and denser dot distribution, and has the potential to be integrated with the existing biological assays for rapid and sensitive detection of foodborne bacteria. |
format | Online Article Text |
id | pubmed-6315333 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63153332019-01-10 A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains Cai, Gaozhe Wang, Siyuan Zheng, Lingyan Lin, Jianhan Micromachines (Basel) Article Immunomagnetic separation has been widely used for the separation and concentration of foodborne pathogens from complex food samples, however it can only handle a small volume of samples. In this paper, we presented a novel fluidic device for the specific and efficient separation and concentration of salmonella typhimurium using self-assembled magnetic nanoparticle chains. The laminated sawtooth-shaped iron foils were first mounted in the 3D-printed matrix and magnetized by a strong magnet to generate dot-array high gradient magnetic fields in the fluidic channel, which was simulated using COMSOL (5.3a, Burlington, MA, USA). Then, magnetic nanoparticles with a diameter of 150 nm, which were modified with the anti-salmonella polyclonal antibodies, were injected into the channel, and the magnetic nanoparticle chains were vertically formed at the dots and verified using a fluorescence inverted microscope. Finally, the bacterial sample was continuous-flow injected, and the target bacteria could be captured by the antibodies on the chains, followed by gold standard culture plating to determine the amount of the target bacteria. Under the optimal conditions, the target bacteria could be separated with a separation efficiency of 80% in 45 min. This fluidic device could be further improved using thinner sawtooth-shaped iron foils and stronger magnets to obtain a better dot-array magnetic field with larger magnetic intensity and denser dot distribution, and has the potential to be integrated with the existing biological assays for rapid and sensitive detection of foodborne bacteria. MDPI 2018-11-27 /pmc/articles/PMC6315333/ /pubmed/30486364 http://dx.doi.org/10.3390/mi9120624 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cai, Gaozhe Wang, Siyuan Zheng, Lingyan Lin, Jianhan A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains |
title | A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains |
title_full | A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains |
title_fullStr | A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains |
title_full_unstemmed | A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains |
title_short | A Fluidic Device for Immunomagnetic Separation of Foodborne Bacteria Using Self-Assembled Magnetic Nanoparticle Chains |
title_sort | fluidic device for immunomagnetic separation of foodborne bacteria using self-assembled magnetic nanoparticle chains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315333/ https://www.ncbi.nlm.nih.gov/pubmed/30486364 http://dx.doi.org/10.3390/mi9120624 |
work_keys_str_mv | AT caigaozhe afluidicdeviceforimmunomagneticseparationoffoodbornebacteriausingselfassembledmagneticnanoparticlechains AT wangsiyuan afluidicdeviceforimmunomagneticseparationoffoodbornebacteriausingselfassembledmagneticnanoparticlechains AT zhenglingyan afluidicdeviceforimmunomagneticseparationoffoodbornebacteriausingselfassembledmagneticnanoparticlechains AT linjianhan afluidicdeviceforimmunomagneticseparationoffoodbornebacteriausingselfassembledmagneticnanoparticlechains AT caigaozhe fluidicdeviceforimmunomagneticseparationoffoodbornebacteriausingselfassembledmagneticnanoparticlechains AT wangsiyuan fluidicdeviceforimmunomagneticseparationoffoodbornebacteriausingselfassembledmagneticnanoparticlechains AT zhenglingyan fluidicdeviceforimmunomagneticseparationoffoodbornebacteriausingselfassembledmagneticnanoparticlechains AT linjianhan fluidicdeviceforimmunomagneticseparationoffoodbornebacteriausingselfassembledmagneticnanoparticlechains |