Cargando…
Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells
Host defense peptides (HDPs) are efficient defense components of the innate immune system, playing critical roles in intestinal homeostasis and protection against pathogens. This study aims to investigate the interference effects of DON on the intestinal porcine HDPs expression in piglets and intest...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315515/ https://www.ncbi.nlm.nih.gov/pubmed/30558299 http://dx.doi.org/10.3390/toxins10120541 |
_version_ | 1783384313198280704 |
---|---|
author | Wang, Shuai Yang, Jiacheng Zhang, Beiyu Wu, Kuntan Yang, Ao Li, Chong Zhang, Jiacai Zhang, Cong Rajput, Shahid Ali Zhang, Niya Sun, Lvhui Qi, Desheng |
author_facet | Wang, Shuai Yang, Jiacheng Zhang, Beiyu Wu, Kuntan Yang, Ao Li, Chong Zhang, Jiacai Zhang, Cong Rajput, Shahid Ali Zhang, Niya Sun, Lvhui Qi, Desheng |
author_sort | Wang, Shuai |
collection | PubMed |
description | Host defense peptides (HDPs) are efficient defense components of the innate immune system, playing critical roles in intestinal homeostasis and protection against pathogens. This study aims to investigate the interference effects of DON on the intestinal porcine HDPs expression in piglets and intestinal porcine epithelial cell line (IPEC-J2) cells, and elucidate the underlying mechanisms through which it functions. In an animal experiment, intestinal HDPs were determined in weaned piglets fed control and 1.28 mg/kg or 2.89 mg/kg DON-contaminated diets. Dietary exposure to DON significantly decreased piglet average daily gain, increased intestinal permeability and depressed the expression of porcine β-defensin1 (pBD1), pBD2, pBD3, epididymis protein 2 splicing variant C (pEP2C), PMAP23, and proline/arginine-rich peptide of 39 amino acids (PR39) in the intestine (p < 0.05). In IPEC-J2 cells, DON decreased cell viability and inhibited the expression of pBD1, pBD3, pEP2C, PG1-5, and PR39 (p < 0.05). NOD2, key regulator that is responsible for HDPs production, was markedly downregulated, whereas caspase-12 was activated in the presence of DON. In conclusion, DON induced caspase-12 activation and inhibited the NOD2-mediated HDPs production, which led to an impaired intestinal barrier integrity of weaned piglets. Our study provides a promising target for future therapeutic strategies to prevent the adverse effects of DON. |
format | Online Article Text |
id | pubmed-6315515 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63155152019-01-11 Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells Wang, Shuai Yang, Jiacheng Zhang, Beiyu Wu, Kuntan Yang, Ao Li, Chong Zhang, Jiacai Zhang, Cong Rajput, Shahid Ali Zhang, Niya Sun, Lvhui Qi, Desheng Toxins (Basel) Article Host defense peptides (HDPs) are efficient defense components of the innate immune system, playing critical roles in intestinal homeostasis and protection against pathogens. This study aims to investigate the interference effects of DON on the intestinal porcine HDPs expression in piglets and intestinal porcine epithelial cell line (IPEC-J2) cells, and elucidate the underlying mechanisms through which it functions. In an animal experiment, intestinal HDPs were determined in weaned piglets fed control and 1.28 mg/kg or 2.89 mg/kg DON-contaminated diets. Dietary exposure to DON significantly decreased piglet average daily gain, increased intestinal permeability and depressed the expression of porcine β-defensin1 (pBD1), pBD2, pBD3, epididymis protein 2 splicing variant C (pEP2C), PMAP23, and proline/arginine-rich peptide of 39 amino acids (PR39) in the intestine (p < 0.05). In IPEC-J2 cells, DON decreased cell viability and inhibited the expression of pBD1, pBD3, pEP2C, PG1-5, and PR39 (p < 0.05). NOD2, key regulator that is responsible for HDPs production, was markedly downregulated, whereas caspase-12 was activated in the presence of DON. In conclusion, DON induced caspase-12 activation and inhibited the NOD2-mediated HDPs production, which led to an impaired intestinal barrier integrity of weaned piglets. Our study provides a promising target for future therapeutic strategies to prevent the adverse effects of DON. MDPI 2018-12-15 /pmc/articles/PMC6315515/ /pubmed/30558299 http://dx.doi.org/10.3390/toxins10120541 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Shuai Yang, Jiacheng Zhang, Beiyu Wu, Kuntan Yang, Ao Li, Chong Zhang, Jiacai Zhang, Cong Rajput, Shahid Ali Zhang, Niya Sun, Lvhui Qi, Desheng Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells |
title | Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells |
title_full | Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells |
title_fullStr | Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells |
title_full_unstemmed | Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells |
title_short | Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells |
title_sort | deoxynivalenol impairs porcine intestinal host defense peptide expression in weaned piglets and ipec-j2 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315515/ https://www.ncbi.nlm.nih.gov/pubmed/30558299 http://dx.doi.org/10.3390/toxins10120541 |
work_keys_str_mv | AT wangshuai deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT yangjiacheng deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT zhangbeiyu deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT wukuntan deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT yangao deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT lichong deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT zhangjiacai deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT zhangcong deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT rajputshahidali deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT zhangniya deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT sunlvhui deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells AT qidesheng deoxynivalenolimpairsporcineintestinalhostdefensepeptideexpressioninweanedpigletsandipecj2cells |