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Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells

Host defense peptides (HDPs) are efficient defense components of the innate immune system, playing critical roles in intestinal homeostasis and protection against pathogens. This study aims to investigate the interference effects of DON on the intestinal porcine HDPs expression in piglets and intest...

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Autores principales: Wang, Shuai, Yang, Jiacheng, Zhang, Beiyu, Wu, Kuntan, Yang, Ao, Li, Chong, Zhang, Jiacai, Zhang, Cong, Rajput, Shahid Ali, Zhang, Niya, Sun, Lvhui, Qi, Desheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315515/
https://www.ncbi.nlm.nih.gov/pubmed/30558299
http://dx.doi.org/10.3390/toxins10120541
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author Wang, Shuai
Yang, Jiacheng
Zhang, Beiyu
Wu, Kuntan
Yang, Ao
Li, Chong
Zhang, Jiacai
Zhang, Cong
Rajput, Shahid Ali
Zhang, Niya
Sun, Lvhui
Qi, Desheng
author_facet Wang, Shuai
Yang, Jiacheng
Zhang, Beiyu
Wu, Kuntan
Yang, Ao
Li, Chong
Zhang, Jiacai
Zhang, Cong
Rajput, Shahid Ali
Zhang, Niya
Sun, Lvhui
Qi, Desheng
author_sort Wang, Shuai
collection PubMed
description Host defense peptides (HDPs) are efficient defense components of the innate immune system, playing critical roles in intestinal homeostasis and protection against pathogens. This study aims to investigate the interference effects of DON on the intestinal porcine HDPs expression in piglets and intestinal porcine epithelial cell line (IPEC-J2) cells, and elucidate the underlying mechanisms through which it functions. In an animal experiment, intestinal HDPs were determined in weaned piglets fed control and 1.28 mg/kg or 2.89 mg/kg DON-contaminated diets. Dietary exposure to DON significantly decreased piglet average daily gain, increased intestinal permeability and depressed the expression of porcine β-defensin1 (pBD1), pBD2, pBD3, epididymis protein 2 splicing variant C (pEP2C), PMAP23, and proline/arginine-rich peptide of 39 amino acids (PR39) in the intestine (p < 0.05). In IPEC-J2 cells, DON decreased cell viability and inhibited the expression of pBD1, pBD3, pEP2C, PG1-5, and PR39 (p < 0.05). NOD2, key regulator that is responsible for HDPs production, was markedly downregulated, whereas caspase-12 was activated in the presence of DON. In conclusion, DON induced caspase-12 activation and inhibited the NOD2-mediated HDPs production, which led to an impaired intestinal barrier integrity of weaned piglets. Our study provides a promising target for future therapeutic strategies to prevent the adverse effects of DON.
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spelling pubmed-63155152019-01-11 Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells Wang, Shuai Yang, Jiacheng Zhang, Beiyu Wu, Kuntan Yang, Ao Li, Chong Zhang, Jiacai Zhang, Cong Rajput, Shahid Ali Zhang, Niya Sun, Lvhui Qi, Desheng Toxins (Basel) Article Host defense peptides (HDPs) are efficient defense components of the innate immune system, playing critical roles in intestinal homeostasis and protection against pathogens. This study aims to investigate the interference effects of DON on the intestinal porcine HDPs expression in piglets and intestinal porcine epithelial cell line (IPEC-J2) cells, and elucidate the underlying mechanisms through which it functions. In an animal experiment, intestinal HDPs were determined in weaned piglets fed control and 1.28 mg/kg or 2.89 mg/kg DON-contaminated diets. Dietary exposure to DON significantly decreased piglet average daily gain, increased intestinal permeability and depressed the expression of porcine β-defensin1 (pBD1), pBD2, pBD3, epididymis protein 2 splicing variant C (pEP2C), PMAP23, and proline/arginine-rich peptide of 39 amino acids (PR39) in the intestine (p < 0.05). In IPEC-J2 cells, DON decreased cell viability and inhibited the expression of pBD1, pBD3, pEP2C, PG1-5, and PR39 (p < 0.05). NOD2, key regulator that is responsible for HDPs production, was markedly downregulated, whereas caspase-12 was activated in the presence of DON. In conclusion, DON induced caspase-12 activation and inhibited the NOD2-mediated HDPs production, which led to an impaired intestinal barrier integrity of weaned piglets. Our study provides a promising target for future therapeutic strategies to prevent the adverse effects of DON. MDPI 2018-12-15 /pmc/articles/PMC6315515/ /pubmed/30558299 http://dx.doi.org/10.3390/toxins10120541 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Shuai
Yang, Jiacheng
Zhang, Beiyu
Wu, Kuntan
Yang, Ao
Li, Chong
Zhang, Jiacai
Zhang, Cong
Rajput, Shahid Ali
Zhang, Niya
Sun, Lvhui
Qi, Desheng
Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells
title Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells
title_full Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells
title_fullStr Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells
title_full_unstemmed Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells
title_short Deoxynivalenol Impairs Porcine Intestinal Host Defense Peptide Expression in Weaned Piglets and IPEC-J2 Cells
title_sort deoxynivalenol impairs porcine intestinal host defense peptide expression in weaned piglets and ipec-j2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315515/
https://www.ncbi.nlm.nih.gov/pubmed/30558299
http://dx.doi.org/10.3390/toxins10120541
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