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PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures
Low temperature cofired ceramics (LTCC) provide a technology for the 3-dimensional integration of sensor arrays into bioreactors covering dimensions of several hundred micrometers. Since optical control in such assemblies is not possible, the in situ detection of cell adhesion on impedance electrode...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315667/ https://www.ncbi.nlm.nih.gov/pubmed/30400265 http://dx.doi.org/10.3390/bios8040105 |
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author | Bartsch, Heike Peipmann, Ralf Klett, Maren Brauer, Dana Schober, Andreas Müller, Jens |
author_facet | Bartsch, Heike Peipmann, Ralf Klett, Maren Brauer, Dana Schober, Andreas Müller, Jens |
author_sort | Bartsch, Heike |
collection | PubMed |
description | Low temperature cofired ceramics (LTCC) provide a technology for the 3-dimensional integration of sensor arrays into bioreactors covering dimensions of several hundred micrometers. Since optical control in such assemblies is not possible, the in situ detection of cell adhesion on impedance electrodes with high spatial resolution would deliver crucial information. A current limitation is the increasing impedance of microelectrodes with decreasing diameter. This study evaluates the suitability of thick film gold electrodes, pristine and coated with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT), for the detection of cell adhesion on the electrode surface. The impedance as criterion for cell attachment is measured with a recording system for electroactive cells with the aim of improving usability. Two cell cultures with different adhesion characteristic are used for adhesion assessment on planar test chips. The impedance increase measured on individual PEDOT coated electrodes due to tight contact of cells reaches a factor of 6.8 in cultures of well-adherent HepG2 cells. Less adhered NG108-15 cells produce a maximum impedance increase by a factor of 2.6. Since the electrode impedance is significantly reduced by PEDOT coating, a reduction of the electrode diameter to values below 100 µm and spatially resolved detection is possible. The results encourage further studies using PEDOT coated thick film electrodes as bio-electronic-interfaces. We presume that such miniaturized electrodes are suitable for 3-dimensional recordings in electroactive cell cultures, providing information of local cell adhesion at the same time. |
format | Online Article Text |
id | pubmed-6315667 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63156672019-01-09 PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures Bartsch, Heike Peipmann, Ralf Klett, Maren Brauer, Dana Schober, Andreas Müller, Jens Biosensors (Basel) Article Low temperature cofired ceramics (LTCC) provide a technology for the 3-dimensional integration of sensor arrays into bioreactors covering dimensions of several hundred micrometers. Since optical control in such assemblies is not possible, the in situ detection of cell adhesion on impedance electrodes with high spatial resolution would deliver crucial information. A current limitation is the increasing impedance of microelectrodes with decreasing diameter. This study evaluates the suitability of thick film gold electrodes, pristine and coated with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT), for the detection of cell adhesion on the electrode surface. The impedance as criterion for cell attachment is measured with a recording system for electroactive cells with the aim of improving usability. Two cell cultures with different adhesion characteristic are used for adhesion assessment on planar test chips. The impedance increase measured on individual PEDOT coated electrodes due to tight contact of cells reaches a factor of 6.8 in cultures of well-adherent HepG2 cells. Less adhered NG108-15 cells produce a maximum impedance increase by a factor of 2.6. Since the electrode impedance is significantly reduced by PEDOT coating, a reduction of the electrode diameter to values below 100 µm and spatially resolved detection is possible. The results encourage further studies using PEDOT coated thick film electrodes as bio-electronic-interfaces. We presume that such miniaturized electrodes are suitable for 3-dimensional recordings in electroactive cell cultures, providing information of local cell adhesion at the same time. MDPI 2018-11-02 /pmc/articles/PMC6315667/ /pubmed/30400265 http://dx.doi.org/10.3390/bios8040105 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bartsch, Heike Peipmann, Ralf Klett, Maren Brauer, Dana Schober, Andreas Müller, Jens PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures |
title | PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures |
title_full | PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures |
title_fullStr | PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures |
title_full_unstemmed | PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures |
title_short | PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures |
title_sort | pedot coated thick film electrodes for in situ detection of cell adhesion in cell cultures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315667/ https://www.ncbi.nlm.nih.gov/pubmed/30400265 http://dx.doi.org/10.3390/bios8040105 |
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