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PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures

Low temperature cofired ceramics (LTCC) provide a technology for the 3-dimensional integration of sensor arrays into bioreactors covering dimensions of several hundred micrometers. Since optical control in such assemblies is not possible, the in situ detection of cell adhesion on impedance electrode...

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Autores principales: Bartsch, Heike, Peipmann, Ralf, Klett, Maren, Brauer, Dana, Schober, Andreas, Müller, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315667/
https://www.ncbi.nlm.nih.gov/pubmed/30400265
http://dx.doi.org/10.3390/bios8040105
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author Bartsch, Heike
Peipmann, Ralf
Klett, Maren
Brauer, Dana
Schober, Andreas
Müller, Jens
author_facet Bartsch, Heike
Peipmann, Ralf
Klett, Maren
Brauer, Dana
Schober, Andreas
Müller, Jens
author_sort Bartsch, Heike
collection PubMed
description Low temperature cofired ceramics (LTCC) provide a technology for the 3-dimensional integration of sensor arrays into bioreactors covering dimensions of several hundred micrometers. Since optical control in such assemblies is not possible, the in situ detection of cell adhesion on impedance electrodes with high spatial resolution would deliver crucial information. A current limitation is the increasing impedance of microelectrodes with decreasing diameter. This study evaluates the suitability of thick film gold electrodes, pristine and coated with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT), for the detection of cell adhesion on the electrode surface. The impedance as criterion for cell attachment is measured with a recording system for electroactive cells with the aim of improving usability. Two cell cultures with different adhesion characteristic are used for adhesion assessment on planar test chips. The impedance increase measured on individual PEDOT coated electrodes due to tight contact of cells reaches a factor of 6.8 in cultures of well-adherent HepG2 cells. Less adhered NG108-15 cells produce a maximum impedance increase by a factor of 2.6. Since the electrode impedance is significantly reduced by PEDOT coating, a reduction of the electrode diameter to values below 100 µm and spatially resolved detection is possible. The results encourage further studies using PEDOT coated thick film electrodes as bio-electronic-interfaces. We presume that such miniaturized electrodes are suitable for 3-dimensional recordings in electroactive cell cultures, providing information of local cell adhesion at the same time.
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spelling pubmed-63156672019-01-09 PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures Bartsch, Heike Peipmann, Ralf Klett, Maren Brauer, Dana Schober, Andreas Müller, Jens Biosensors (Basel) Article Low temperature cofired ceramics (LTCC) provide a technology for the 3-dimensional integration of sensor arrays into bioreactors covering dimensions of several hundred micrometers. Since optical control in such assemblies is not possible, the in situ detection of cell adhesion on impedance electrodes with high spatial resolution would deliver crucial information. A current limitation is the increasing impedance of microelectrodes with decreasing diameter. This study evaluates the suitability of thick film gold electrodes, pristine and coated with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT), for the detection of cell adhesion on the electrode surface. The impedance as criterion for cell attachment is measured with a recording system for electroactive cells with the aim of improving usability. Two cell cultures with different adhesion characteristic are used for adhesion assessment on planar test chips. The impedance increase measured on individual PEDOT coated electrodes due to tight contact of cells reaches a factor of 6.8 in cultures of well-adherent HepG2 cells. Less adhered NG108-15 cells produce a maximum impedance increase by a factor of 2.6. Since the electrode impedance is significantly reduced by PEDOT coating, a reduction of the electrode diameter to values below 100 µm and spatially resolved detection is possible. The results encourage further studies using PEDOT coated thick film electrodes as bio-electronic-interfaces. We presume that such miniaturized electrodes are suitable for 3-dimensional recordings in electroactive cell cultures, providing information of local cell adhesion at the same time. MDPI 2018-11-02 /pmc/articles/PMC6315667/ /pubmed/30400265 http://dx.doi.org/10.3390/bios8040105 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bartsch, Heike
Peipmann, Ralf
Klett, Maren
Brauer, Dana
Schober, Andreas
Müller, Jens
PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures
title PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures
title_full PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures
title_fullStr PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures
title_full_unstemmed PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures
title_short PEDOT Coated Thick Film Electrodes for In Situ Detection of Cell Adhesion in Cell Cultures
title_sort pedot coated thick film electrodes for in situ detection of cell adhesion in cell cultures
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315667/
https://www.ncbi.nlm.nih.gov/pubmed/30400265
http://dx.doi.org/10.3390/bios8040105
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