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Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae

Coping with daily and seasonal temperature fluctuations is a key adaptive process for species to colonize temperate regions all over the globe. Over the past 18,000 years, the tropical species Drosophila ananassae expanded its home range from tropical regions in Southeast Asia to more temperate regi...

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Detalles Bibliográficos
Autores principales: Königer, Annabella, Grath, Sonja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315829/
https://www.ncbi.nlm.nih.gov/pubmed/30545157
http://dx.doi.org/10.3390/genes9120624
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author Königer, Annabella
Grath, Sonja
author_facet Königer, Annabella
Grath, Sonja
author_sort Königer, Annabella
collection PubMed
description Coping with daily and seasonal temperature fluctuations is a key adaptive process for species to colonize temperate regions all over the globe. Over the past 18,000 years, the tropical species Drosophila ananassae expanded its home range from tropical regions in Southeast Asia to more temperate regions. Phenotypic assays of chill coma recovery time (CCRT) together with previously published population genetic data suggest that only a small number of genes underlie improved cold hardiness in the cold-adapted populations. We used high-throughput RNA sequencing to analyze differential gene expression before and after exposure to a cold shock in cold-tolerant lines (those with fast chill coma recovery, CCR) and cold-sensitive lines (slow CCR) from a population originating from Bangkok, Thailand (the ancestral species range). We identified two candidate genes with a significant interaction between cold tolerance and cold shock treatment: GF14647 and GF15058. Further, our data suggest that selection for increased cold tolerance did not operate through the increased activity of heat shock proteins, but more likely through the stabilization of the actin cytoskeleton and a delayed onset of apoptosis.
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spelling pubmed-63158292019-01-09 Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae Königer, Annabella Grath, Sonja Genes (Basel) Article Coping with daily and seasonal temperature fluctuations is a key adaptive process for species to colonize temperate regions all over the globe. Over the past 18,000 years, the tropical species Drosophila ananassae expanded its home range from tropical regions in Southeast Asia to more temperate regions. Phenotypic assays of chill coma recovery time (CCRT) together with previously published population genetic data suggest that only a small number of genes underlie improved cold hardiness in the cold-adapted populations. We used high-throughput RNA sequencing to analyze differential gene expression before and after exposure to a cold shock in cold-tolerant lines (those with fast chill coma recovery, CCR) and cold-sensitive lines (slow CCR) from a population originating from Bangkok, Thailand (the ancestral species range). We identified two candidate genes with a significant interaction between cold tolerance and cold shock treatment: GF14647 and GF15058. Further, our data suggest that selection for increased cold tolerance did not operate through the increased activity of heat shock proteins, but more likely through the stabilization of the actin cytoskeleton and a delayed onset of apoptosis. MDPI 2018-12-12 /pmc/articles/PMC6315829/ /pubmed/30545157 http://dx.doi.org/10.3390/genes9120624 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Königer, Annabella
Grath, Sonja
Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae
title Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae
title_full Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae
title_fullStr Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae
title_full_unstemmed Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae
title_short Transcriptome Analysis Reveals Candidate Genes for Cold Tolerance in Drosophila ananassae
title_sort transcriptome analysis reveals candidate genes for cold tolerance in drosophila ananassae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315829/
https://www.ncbi.nlm.nih.gov/pubmed/30545157
http://dx.doi.org/10.3390/genes9120624
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