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Effects of Alternative Blood Sources on Wolbachia Infected Aedes aegypti Females within and across Generations

Wolbachia bacteria have been identified as a tool for reducing the transmission of arboviruses transmitted by Aedes aegypti. Research groups around the world are now mass rearing Wolbachia-infected Ae. aegypti for deliberate release. We investigated the fitness impact of a crucial element of mass re...

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Autores principales: Paris, Véronique, Cottingham, Ellen, Ross, Perran A., Axford, Jason K., Hoffmann, Ary A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315918/
https://www.ncbi.nlm.nih.gov/pubmed/30314399
http://dx.doi.org/10.3390/insects9040140
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author Paris, Véronique
Cottingham, Ellen
Ross, Perran A.
Axford, Jason K.
Hoffmann, Ary A.
author_facet Paris, Véronique
Cottingham, Ellen
Ross, Perran A.
Axford, Jason K.
Hoffmann, Ary A.
author_sort Paris, Véronique
collection PubMed
description Wolbachia bacteria have been identified as a tool for reducing the transmission of arboviruses transmitted by Aedes aegypti. Research groups around the world are now mass rearing Wolbachia-infected Ae. aegypti for deliberate release. We investigated the fitness impact of a crucial element of mass rearing: the blood meal required by female Ae. aegypti to lay eggs. Although Ae. aegypti almost exclusively feed on human blood, it is often difficult to use human blood in disease-endemic settings. When females were fed on sheep or pig blood rather than human blood, egg hatch rates decreased in all three lines tested (uninfected, or infected by wMel, or wAlbB Wolbachia). This finding was particularly pronounced when fed on sheep blood, although fecundity was not affected. Some of these effects persisted after an additional generation on human blood. Attempts to keep populations on sheep and pig blood sources only partly succeeded, suggesting that strong adaptation is required to develop a stably infected line on an alternative blood source. There was a decrease in Wolbachia density when Ae. aegypti were fed on non-human blood sources. Density increased in lines kept for multiple generations on the alternate sources but was still reduced relative to lines kept on human blood. These findings suggest that sheep and pig blood will entail a cost when used for maintaining Wolbachia-infected Ae. aegypti. These costs should be taken into account when planning mass release programs.
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spelling pubmed-63159182019-05-05 Effects of Alternative Blood Sources on Wolbachia Infected Aedes aegypti Females within and across Generations Paris, Véronique Cottingham, Ellen Ross, Perran A. Axford, Jason K. Hoffmann, Ary A. Insects Article Wolbachia bacteria have been identified as a tool for reducing the transmission of arboviruses transmitted by Aedes aegypti. Research groups around the world are now mass rearing Wolbachia-infected Ae. aegypti for deliberate release. We investigated the fitness impact of a crucial element of mass rearing: the blood meal required by female Ae. aegypti to lay eggs. Although Ae. aegypti almost exclusively feed on human blood, it is often difficult to use human blood in disease-endemic settings. When females were fed on sheep or pig blood rather than human blood, egg hatch rates decreased in all three lines tested (uninfected, or infected by wMel, or wAlbB Wolbachia). This finding was particularly pronounced when fed on sheep blood, although fecundity was not affected. Some of these effects persisted after an additional generation on human blood. Attempts to keep populations on sheep and pig blood sources only partly succeeded, suggesting that strong adaptation is required to develop a stably infected line on an alternative blood source. There was a decrease in Wolbachia density when Ae. aegypti were fed on non-human blood sources. Density increased in lines kept for multiple generations on the alternate sources but was still reduced relative to lines kept on human blood. These findings suggest that sheep and pig blood will entail a cost when used for maintaining Wolbachia-infected Ae. aegypti. These costs should be taken into account when planning mass release programs. MDPI 2018-10-11 /pmc/articles/PMC6315918/ /pubmed/30314399 http://dx.doi.org/10.3390/insects9040140 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paris, Véronique
Cottingham, Ellen
Ross, Perran A.
Axford, Jason K.
Hoffmann, Ary A.
Effects of Alternative Blood Sources on Wolbachia Infected Aedes aegypti Females within and across Generations
title Effects of Alternative Blood Sources on Wolbachia Infected Aedes aegypti Females within and across Generations
title_full Effects of Alternative Blood Sources on Wolbachia Infected Aedes aegypti Females within and across Generations
title_fullStr Effects of Alternative Blood Sources on Wolbachia Infected Aedes aegypti Females within and across Generations
title_full_unstemmed Effects of Alternative Blood Sources on Wolbachia Infected Aedes aegypti Females within and across Generations
title_short Effects of Alternative Blood Sources on Wolbachia Infected Aedes aegypti Females within and across Generations
title_sort effects of alternative blood sources on wolbachia infected aedes aegypti females within and across generations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315918/
https://www.ncbi.nlm.nih.gov/pubmed/30314399
http://dx.doi.org/10.3390/insects9040140
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