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Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis
A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patient...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315978/ https://www.ncbi.nlm.nih.gov/pubmed/30545099 http://dx.doi.org/10.3390/bios8040128 |
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author | Gordillo-Marroquín, Cristina Gómez-Velasco, Anaximandro Sánchez-Pérez, Héctor J. Pryg, Kasey Shinners, John Murray, Nathan Muñoz-Jiménez, Sergio G. Bencomo-Alerm, Allied Gómez-Bustamante, Adriana Jonapá-Gómez, Letisia Enríquez-Ríos, Natán Martín, Miguel Romero-Sandoval, Natalia Alocilja, Evangelyn C. |
author_facet | Gordillo-Marroquín, Cristina Gómez-Velasco, Anaximandro Sánchez-Pérez, Héctor J. Pryg, Kasey Shinners, John Murray, Nathan Muñoz-Jiménez, Sergio G. Bencomo-Alerm, Allied Gómez-Bustamante, Adriana Jonapá-Gómez, Letisia Enríquez-Ríos, Natán Martín, Miguel Romero-Sandoval, Natalia Alocilja, Evangelyn C. |
author_sort | Gordillo-Marroquín, Cristina |
collection | PubMed |
description | A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1+” (in SSM) to “2+”. Extending the finding to paucibacillary cases, there is the likelihood of a “scant” grade to become “1+”. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs. |
format | Online Article Text |
id | pubmed-6315978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63159782019-01-09 Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis Gordillo-Marroquín, Cristina Gómez-Velasco, Anaximandro Sánchez-Pérez, Héctor J. Pryg, Kasey Shinners, John Murray, Nathan Muñoz-Jiménez, Sergio G. Bencomo-Alerm, Allied Gómez-Bustamante, Adriana Jonapá-Gómez, Letisia Enríquez-Ríos, Natán Martín, Miguel Romero-Sandoval, Natalia Alocilja, Evangelyn C. Biosensors (Basel) Article A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1+” (in SSM) to “2+”. Extending the finding to paucibacillary cases, there is the likelihood of a “scant” grade to become “1+”. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs. MDPI 2018-12-12 /pmc/articles/PMC6315978/ /pubmed/30545099 http://dx.doi.org/10.3390/bios8040128 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gordillo-Marroquín, Cristina Gómez-Velasco, Anaximandro Sánchez-Pérez, Héctor J. Pryg, Kasey Shinners, John Murray, Nathan Muñoz-Jiménez, Sergio G. Bencomo-Alerm, Allied Gómez-Bustamante, Adriana Jonapá-Gómez, Letisia Enríquez-Ríos, Natán Martín, Miguel Romero-Sandoval, Natalia Alocilja, Evangelyn C. Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
title | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
title_full | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
title_fullStr | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
title_full_unstemmed | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
title_short | Magnetic Nanoparticle-Based Biosensing Assay Quantitatively Enhances Acid-Fast Bacilli Count in Paucibacillary Pulmonary Tuberculosis |
title_sort | magnetic nanoparticle-based biosensing assay quantitatively enhances acid-fast bacilli count in paucibacillary pulmonary tuberculosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315978/ https://www.ncbi.nlm.nih.gov/pubmed/30545099 http://dx.doi.org/10.3390/bios8040128 |
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