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Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection

Hemolytic–uremic syndrome is a life-threating disease most often associated with Shiga toxin-producing microorganisms like Escherichia coli (STEC), including E. coli O157:H7. Shiga toxin is encoded by resident prophages present within this bacterium, and both its production and release depend on the...

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Autores principales: Howard-Varona, Cristina, Vik, Dean R., Solonenko, Natalie E., Li, Yueh-Fen, Gazitua, M. Consuelo, Chittick, Lauren, Samiec, Jennifer K., Jensen, Aubrey E., Anderson, Paige, Howard-Varona, Adrian, Kinkhabwala, Anika A., Abedon, Stephen T., Sullivan, Matthew B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315980/
https://www.ncbi.nlm.nih.gov/pubmed/30453470
http://dx.doi.org/10.3390/antibiotics7040101
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author Howard-Varona, Cristina
Vik, Dean R.
Solonenko, Natalie E.
Li, Yueh-Fen
Gazitua, M. Consuelo
Chittick, Lauren
Samiec, Jennifer K.
Jensen, Aubrey E.
Anderson, Paige
Howard-Varona, Adrian
Kinkhabwala, Anika A.
Abedon, Stephen T.
Sullivan, Matthew B.
author_facet Howard-Varona, Cristina
Vik, Dean R.
Solonenko, Natalie E.
Li, Yueh-Fen
Gazitua, M. Consuelo
Chittick, Lauren
Samiec, Jennifer K.
Jensen, Aubrey E.
Anderson, Paige
Howard-Varona, Adrian
Kinkhabwala, Anika A.
Abedon, Stephen T.
Sullivan, Matthew B.
author_sort Howard-Varona, Cristina
collection PubMed
description Hemolytic–uremic syndrome is a life-threating disease most often associated with Shiga toxin-producing microorganisms like Escherichia coli (STEC), including E. coli O157:H7. Shiga toxin is encoded by resident prophages present within this bacterium, and both its production and release depend on the induction of Shiga toxin-encoding prophages. Consequently, treatment of STEC infections tend to be largely supportive rather than antibacterial, in part due to concerns about exacerbating such prophage induction. Here we explore STEC O157:H7 prophage induction in vitro as it pertains to phage therapy—the application of bacteriophages as antibacterial agents to treat bacterial infections—to curtail prophage induction events, while also reducing STEC O157:H7 presence. We observed that cultures treated with strictly lytic phages, despite being lysed, produce substantially fewer Shiga toxin-encoding temperate-phage virions than untreated STEC controls. We therefore suggest that phage therapy could have utility as a prophylactic treatment of individuals suspected of having been recently exposed to STEC, especially if prophage induction and by extension Shiga toxin production is not exacerbated.
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spelling pubmed-63159802019-01-11 Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection Howard-Varona, Cristina Vik, Dean R. Solonenko, Natalie E. Li, Yueh-Fen Gazitua, M. Consuelo Chittick, Lauren Samiec, Jennifer K. Jensen, Aubrey E. Anderson, Paige Howard-Varona, Adrian Kinkhabwala, Anika A. Abedon, Stephen T. Sullivan, Matthew B. Antibiotics (Basel) Article Hemolytic–uremic syndrome is a life-threating disease most often associated with Shiga toxin-producing microorganisms like Escherichia coli (STEC), including E. coli O157:H7. Shiga toxin is encoded by resident prophages present within this bacterium, and both its production and release depend on the induction of Shiga toxin-encoding prophages. Consequently, treatment of STEC infections tend to be largely supportive rather than antibacterial, in part due to concerns about exacerbating such prophage induction. Here we explore STEC O157:H7 prophage induction in vitro as it pertains to phage therapy—the application of bacteriophages as antibacterial agents to treat bacterial infections—to curtail prophage induction events, while also reducing STEC O157:H7 presence. We observed that cultures treated with strictly lytic phages, despite being lysed, produce substantially fewer Shiga toxin-encoding temperate-phage virions than untreated STEC controls. We therefore suggest that phage therapy could have utility as a prophylactic treatment of individuals suspected of having been recently exposed to STEC, especially if prophage induction and by extension Shiga toxin production is not exacerbated. MDPI 2018-11-16 /pmc/articles/PMC6315980/ /pubmed/30453470 http://dx.doi.org/10.3390/antibiotics7040101 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Howard-Varona, Cristina
Vik, Dean R.
Solonenko, Natalie E.
Li, Yueh-Fen
Gazitua, M. Consuelo
Chittick, Lauren
Samiec, Jennifer K.
Jensen, Aubrey E.
Anderson, Paige
Howard-Varona, Adrian
Kinkhabwala, Anika A.
Abedon, Stephen T.
Sullivan, Matthew B.
Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection
title Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection
title_full Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection
title_fullStr Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection
title_full_unstemmed Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection
title_short Fighting Fire with Fire: Phage Potential for the Treatment of E. coli O157 Infection
title_sort fighting fire with fire: phage potential for the treatment of e. coli o157 infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6315980/
https://www.ncbi.nlm.nih.gov/pubmed/30453470
http://dx.doi.org/10.3390/antibiotics7040101
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