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Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress

Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stre...

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Autores principales: Saelao, Perot, Wang, Ying, Chanthavixay, Ganrea, Yu, Vivian, Gallardo, Rodrigo A., Dekkers, Jack C. M., Lamont, Susan J., Kelly, Terra, Zhou, Huaijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316021/
https://www.ncbi.nlm.nih.gov/pubmed/30486457
http://dx.doi.org/10.3390/genes9120579
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author Saelao, Perot
Wang, Ying
Chanthavixay, Ganrea
Yu, Vivian
Gallardo, Rodrigo A.
Dekkers, Jack C. M.
Lamont, Susan J.
Kelly, Terra
Zhou, Huaijun
author_facet Saelao, Perot
Wang, Ying
Chanthavixay, Ganrea
Yu, Vivian
Gallardo, Rodrigo A.
Dekkers, Jack C. M.
Lamont, Susan J.
Kelly, Terra
Zhou, Huaijun
author_sort Saelao, Perot
collection PubMed
description Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stress, in order to understand the genetic mechanisms of resistance during high ambient temperatures. Fayoumis, which are relatively more resistant to pathogens than Leghorns, had larger numbers of differentially expressed genes (DEGs) during the early stages of infection when compared to Leghorns and subsequently down-regulated their immune response at the latter stages to return to homeostasis. Leghorns had very few DEGs across all observed time points, with the majority of DEGs involved with metabolic and glucose-related functions. Proteomic analysis corroborates findings made within Leghorns, while also identifying interesting candidate genes missed by expression profiling. Poor correlation between changes observed in the proteomic and transcriptomic datasets highlights the potential importance of integrative approaches to understand the mechanisms of disease response. Overall, this study provides novel insights into global protein and expression profiles of these two genetic lines, and provides potential genetic targets involved with NDV resistance during heat stress in poultry.
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spelling pubmed-63160212019-01-09 Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress Saelao, Perot Wang, Ying Chanthavixay, Ganrea Yu, Vivian Gallardo, Rodrigo A. Dekkers, Jack C. M. Lamont, Susan J. Kelly, Terra Zhou, Huaijun Genes (Basel) Article Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stress, in order to understand the genetic mechanisms of resistance during high ambient temperatures. Fayoumis, which are relatively more resistant to pathogens than Leghorns, had larger numbers of differentially expressed genes (DEGs) during the early stages of infection when compared to Leghorns and subsequently down-regulated their immune response at the latter stages to return to homeostasis. Leghorns had very few DEGs across all observed time points, with the majority of DEGs involved with metabolic and glucose-related functions. Proteomic analysis corroborates findings made within Leghorns, while also identifying interesting candidate genes missed by expression profiling. Poor correlation between changes observed in the proteomic and transcriptomic datasets highlights the potential importance of integrative approaches to understand the mechanisms of disease response. Overall, this study provides novel insights into global protein and expression profiles of these two genetic lines, and provides potential genetic targets involved with NDV resistance during heat stress in poultry. MDPI 2018-11-27 /pmc/articles/PMC6316021/ /pubmed/30486457 http://dx.doi.org/10.3390/genes9120579 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saelao, Perot
Wang, Ying
Chanthavixay, Ganrea
Yu, Vivian
Gallardo, Rodrigo A.
Dekkers, Jack C. M.
Lamont, Susan J.
Kelly, Terra
Zhou, Huaijun
Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress
title Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress
title_full Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress
title_fullStr Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress
title_full_unstemmed Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress
title_short Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress
title_sort integrated proteomic and transcriptomic analysis of differential expression of chicken lung tissue in response to ndv infection during heat stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316021/
https://www.ncbi.nlm.nih.gov/pubmed/30486457
http://dx.doi.org/10.3390/genes9120579
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