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Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress
Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stre...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316021/ https://www.ncbi.nlm.nih.gov/pubmed/30486457 http://dx.doi.org/10.3390/genes9120579 |
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author | Saelao, Perot Wang, Ying Chanthavixay, Ganrea Yu, Vivian Gallardo, Rodrigo A. Dekkers, Jack C. M. Lamont, Susan J. Kelly, Terra Zhou, Huaijun |
author_facet | Saelao, Perot Wang, Ying Chanthavixay, Ganrea Yu, Vivian Gallardo, Rodrigo A. Dekkers, Jack C. M. Lamont, Susan J. Kelly, Terra Zhou, Huaijun |
author_sort | Saelao, Perot |
collection | PubMed |
description | Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stress, in order to understand the genetic mechanisms of resistance during high ambient temperatures. Fayoumis, which are relatively more resistant to pathogens than Leghorns, had larger numbers of differentially expressed genes (DEGs) during the early stages of infection when compared to Leghorns and subsequently down-regulated their immune response at the latter stages to return to homeostasis. Leghorns had very few DEGs across all observed time points, with the majority of DEGs involved with metabolic and glucose-related functions. Proteomic analysis corroborates findings made within Leghorns, while also identifying interesting candidate genes missed by expression profiling. Poor correlation between changes observed in the proteomic and transcriptomic datasets highlights the potential importance of integrative approaches to understand the mechanisms of disease response. Overall, this study provides novel insights into global protein and expression profiles of these two genetic lines, and provides potential genetic targets involved with NDV resistance during heat stress in poultry. |
format | Online Article Text |
id | pubmed-6316021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63160212019-01-09 Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress Saelao, Perot Wang, Ying Chanthavixay, Ganrea Yu, Vivian Gallardo, Rodrigo A. Dekkers, Jack C. M. Lamont, Susan J. Kelly, Terra Zhou, Huaijun Genes (Basel) Article Newcastle disease virus (NDV) is a devastating worldwide poultry pathogen with major implications for global food security. In this study, two highly inbred and genetically distinct chicken lines, Fayoumis and Leghorns, were exposed to a lentogenic strain of NDV, while under the effects of heat stress, in order to understand the genetic mechanisms of resistance during high ambient temperatures. Fayoumis, which are relatively more resistant to pathogens than Leghorns, had larger numbers of differentially expressed genes (DEGs) during the early stages of infection when compared to Leghorns and subsequently down-regulated their immune response at the latter stages to return to homeostasis. Leghorns had very few DEGs across all observed time points, with the majority of DEGs involved with metabolic and glucose-related functions. Proteomic analysis corroborates findings made within Leghorns, while also identifying interesting candidate genes missed by expression profiling. Poor correlation between changes observed in the proteomic and transcriptomic datasets highlights the potential importance of integrative approaches to understand the mechanisms of disease response. Overall, this study provides novel insights into global protein and expression profiles of these two genetic lines, and provides potential genetic targets involved with NDV resistance during heat stress in poultry. MDPI 2018-11-27 /pmc/articles/PMC6316021/ /pubmed/30486457 http://dx.doi.org/10.3390/genes9120579 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Saelao, Perot Wang, Ying Chanthavixay, Ganrea Yu, Vivian Gallardo, Rodrigo A. Dekkers, Jack C. M. Lamont, Susan J. Kelly, Terra Zhou, Huaijun Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress |
title | Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress |
title_full | Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress |
title_fullStr | Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress |
title_full_unstemmed | Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress |
title_short | Integrated Proteomic and Transcriptomic Analysis of Differential Expression of Chicken Lung Tissue in Response to NDV Infection during Heat Stress |
title_sort | integrated proteomic and transcriptomic analysis of differential expression of chicken lung tissue in response to ndv infection during heat stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316021/ https://www.ncbi.nlm.nih.gov/pubmed/30486457 http://dx.doi.org/10.3390/genes9120579 |
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