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Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink

In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nano...

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Autores principales: Molinari, Judith, Florez, Laura, Medrano, Anahí, Monsalve, Leandro, Ybarra, Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316051/
https://www.ncbi.nlm.nih.gov/pubmed/30445706
http://dx.doi.org/10.3390/bios8040109
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author Molinari, Judith
Florez, Laura
Medrano, Anahí
Monsalve, Leandro
Ybarra, Gabriel
author_facet Molinari, Judith
Florez, Laura
Medrano, Anahí
Monsalve, Leandro
Ybarra, Gabriel
author_sort Molinari, Judith
collection PubMed
description In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.
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spelling pubmed-63160512019-01-09 Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink Molinari, Judith Florez, Laura Medrano, Anahí Monsalve, Leandro Ybarra, Gabriel Biosensors (Basel) Article In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations. MDPI 2018-11-15 /pmc/articles/PMC6316051/ /pubmed/30445706 http://dx.doi.org/10.3390/bios8040109 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Molinari, Judith
Florez, Laura
Medrano, Anahí
Monsalve, Leandro
Ybarra, Gabriel
Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink
title Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink
title_full Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink
title_fullStr Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink
title_full_unstemmed Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink
title_short Electrochemical Determination of β-Lactoglobulin Employing a Polystyrene Bead-Modified Carbon Nanotube Ink
title_sort electrochemical determination of β-lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316051/
https://www.ncbi.nlm.nih.gov/pubmed/30445706
http://dx.doi.org/10.3390/bios8040109
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