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Detection and Analysis of RNA Ribose 2′-O-Methylations: Challenges and Solutions

Ribose 2′-O-methylation is certainly one of the most common RNA modifications found in almost any type of cellular RNA. It decorates transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), small nuclear RNAs (snRNAs) (and most probably small nucleolar RNAs, snoRNAs), as well as regulatory RNAs like microRNAs...

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Autores principales: Motorin, Yuri, Marchand, Virginie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316082/
https://www.ncbi.nlm.nih.gov/pubmed/30567409
http://dx.doi.org/10.3390/genes9120642
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author Motorin, Yuri
Marchand, Virginie
author_facet Motorin, Yuri
Marchand, Virginie
author_sort Motorin, Yuri
collection PubMed
description Ribose 2′-O-methylation is certainly one of the most common RNA modifications found in almost any type of cellular RNA. It decorates transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), small nuclear RNAs (snRNAs) (and most probably small nucleolar RNAs, snoRNAs), as well as regulatory RNAs like microRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs), and finally, eukaryotic messenger RNAs (mRNAs). Due to this exceptional widespread of RNA 2′-O-methylation, considerable efforts were made in order to precisely map these numerous modifications. Extensive studies of RNA 2′-O-methylation were also stimulated by the discovery of C/D-box snoRNA-guided machinery, which insures site-specific modification of hundreds 2′-O-methylated residues in archaeal and eukaryotic rRNAs and some other RNAs. In this brief review we discussed both traditional approaches of RNA biochemistry and also modern deep sequencing-based methods, used for detection/mapping and quantification of RNA 2′-O-methylations.
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spelling pubmed-63160822019-01-09 Detection and Analysis of RNA Ribose 2′-O-Methylations: Challenges and Solutions Motorin, Yuri Marchand, Virginie Genes (Basel) Review Ribose 2′-O-methylation is certainly one of the most common RNA modifications found in almost any type of cellular RNA. It decorates transfer RNAs (tRNAs), ribosomal RNAs (rRNAs), small nuclear RNAs (snRNAs) (and most probably small nucleolar RNAs, snoRNAs), as well as regulatory RNAs like microRNAs (miRNAs) and Piwi-interacting RNAs (piRNAs), and finally, eukaryotic messenger RNAs (mRNAs). Due to this exceptional widespread of RNA 2′-O-methylation, considerable efforts were made in order to precisely map these numerous modifications. Extensive studies of RNA 2′-O-methylation were also stimulated by the discovery of C/D-box snoRNA-guided machinery, which insures site-specific modification of hundreds 2′-O-methylated residues in archaeal and eukaryotic rRNAs and some other RNAs. In this brief review we discussed both traditional approaches of RNA biochemistry and also modern deep sequencing-based methods, used for detection/mapping and quantification of RNA 2′-O-methylations. MDPI 2018-12-18 /pmc/articles/PMC6316082/ /pubmed/30567409 http://dx.doi.org/10.3390/genes9120642 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Motorin, Yuri
Marchand, Virginie
Detection and Analysis of RNA Ribose 2′-O-Methylations: Challenges and Solutions
title Detection and Analysis of RNA Ribose 2′-O-Methylations: Challenges and Solutions
title_full Detection and Analysis of RNA Ribose 2′-O-Methylations: Challenges and Solutions
title_fullStr Detection and Analysis of RNA Ribose 2′-O-Methylations: Challenges and Solutions
title_full_unstemmed Detection and Analysis of RNA Ribose 2′-O-Methylations: Challenges and Solutions
title_short Detection and Analysis of RNA Ribose 2′-O-Methylations: Challenges and Solutions
title_sort detection and analysis of rna ribose 2′-o-methylations: challenges and solutions
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316082/
https://www.ncbi.nlm.nih.gov/pubmed/30567409
http://dx.doi.org/10.3390/genes9120642
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