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Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique

Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid...

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Autores principales: He, Peijun J. W., Katis, Ioannis N., Eason, Robert W., Sones, Collin L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316105/
https://www.ncbi.nlm.nih.gov/pubmed/30347807
http://dx.doi.org/10.3390/bios8040097
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author He, Peijun J. W.
Katis, Ioannis N.
Eason, Robert W.
Sones, Collin L.
author_facet He, Peijun J. W.
Katis, Ioannis N.
Eason, Robert W.
Sones, Collin L.
author_sort He, Peijun J. W.
collection PubMed
description Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure.
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spelling pubmed-63161052019-01-09 Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique He, Peijun J. W. Katis, Ioannis N. Eason, Robert W. Sones, Collin L. Biosensors (Basel) Article Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure. MDPI 2018-10-20 /pmc/articles/PMC6316105/ /pubmed/30347807 http://dx.doi.org/10.3390/bios8040097 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Peijun J. W.
Katis, Ioannis N.
Eason, Robert W.
Sones, Collin L.
Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
title Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
title_full Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
title_fullStr Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
title_full_unstemmed Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
title_short Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
title_sort rapid multiplexed detection on lateral-flow devices using a laser direct-write technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316105/
https://www.ncbi.nlm.nih.gov/pubmed/30347807
http://dx.doi.org/10.3390/bios8040097
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