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Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique
Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316105/ https://www.ncbi.nlm.nih.gov/pubmed/30347807 http://dx.doi.org/10.3390/bios8040097 |
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author | He, Peijun J. W. Katis, Ioannis N. Eason, Robert W. Sones, Collin L. |
author_facet | He, Peijun J. W. Katis, Ioannis N. Eason, Robert W. Sones, Collin L. |
author_sort | He, Peijun J. W. |
collection | PubMed |
description | Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure. |
format | Online Article Text |
id | pubmed-6316105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63161052019-01-09 Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique He, Peijun J. W. Katis, Ioannis N. Eason, Robert W. Sones, Collin L. Biosensors (Basel) Article Paper-based lateral flow devices (LFDs) are regarded as ideal low-cost diagnostic solutions for point-of-care (POC) scenarios that allow rapid detection of a single analyte within a fluidic sample, and have been in common use for a decade. In recent years, there has been an increasing need for rapid and simultaneous detection of multiple analytes present within a single sample and to facilitate this, we report here a novel solution—detection using a multi-path LFD created via the precise partitioning of the single flow-path of a standard LFD using our previously reported laser direct-write (LDW) technique. The multiple flow-paths allow the simultaneous detection of the different analytes individually within each of the parallel channels without any cross-reactivity. The appearance of coloured test lines in individual channels indicates the presence of the different analytes within a sample. We successfully present the use of a LDW-patterned multi-path LFD for multiplexed detection of a biomarker panel comprising C-reactive protein (CRP) and Serum amyloid A-1 (SAA1), used for the diagnosis of bacterial infections. Overall, we demonstrate the use of our LDW technique in the creation of a novel LFD that enables multiplexed detection of two inflammation markers within a single LFD providing a detection protocol that is comparatively more efficient than the standard sequential multiplexing procedure. MDPI 2018-10-20 /pmc/articles/PMC6316105/ /pubmed/30347807 http://dx.doi.org/10.3390/bios8040097 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article He, Peijun J. W. Katis, Ioannis N. Eason, Robert W. Sones, Collin L. Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique |
title | Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique |
title_full | Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique |
title_fullStr | Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique |
title_full_unstemmed | Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique |
title_short | Rapid Multiplexed Detection on Lateral-Flow Devices Using a Laser Direct-Write Technique |
title_sort | rapid multiplexed detection on lateral-flow devices using a laser direct-write technique |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316105/ https://www.ncbi.nlm.nih.gov/pubmed/30347807 http://dx.doi.org/10.3390/bios8040097 |
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