Cargando…

Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress

Heat shock proteins 70 (Hsp70) are required for key cellular processes and responses to environmental changes, however, there are an unknown number of hsp70 gene family members in the large yellow croaker (Larimichthys crocea). In the present study, 17 hsp70 genes were identified through the genome...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Kaida, Xu, Hanxiang, Han, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316144/
https://www.ncbi.nlm.nih.gov/pubmed/30501106
http://dx.doi.org/10.3390/genes9120590
_version_ 1783384460088049664
author Xu, Kaida
Xu, Hanxiang
Han, Zhiqiang
author_facet Xu, Kaida
Xu, Hanxiang
Han, Zhiqiang
author_sort Xu, Kaida
collection PubMed
description Heat shock proteins 70 (Hsp70) are required for key cellular processes and responses to environmental changes, however, there are an unknown number of hsp70 gene family members in the large yellow croaker (Larimichthys crocea). In the present study, 17 hsp70 genes were identified through the genome of the large yellow croaker. These genes are divided into seven evolutionarily distinct groups according to a phylogenetic tree. The orthologs of these hsp70 genes were found in humans and zebrafish. The expression patterns of the hsp70 gene family in the large yellow croaker under cold and heat stress were studied by examining transcriptome data. Six out of 17 genes were significantly unregulated or downregulated after cold or heat stress. There were two genes significantly upregulated and two genes downregulated in the liver after cold treatment, while after heat treatment, five genes were significantly upregulated, and no genes were significantly downregulated. Three expression patterns were detected: strictly heat-inducible hsp70, constitutively expressed and moderately heat-inducible hsp70, and constitutively expressed and less stress-dependent hsp70 genes. All the findings will contribute to a better understanding of the biological function of hsp70s in defending against thermal challenges.
format Online
Article
Text
id pubmed-6316144
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63161442019-01-09 Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress Xu, Kaida Xu, Hanxiang Han, Zhiqiang Genes (Basel) Article Heat shock proteins 70 (Hsp70) are required for key cellular processes and responses to environmental changes, however, there are an unknown number of hsp70 gene family members in the large yellow croaker (Larimichthys crocea). In the present study, 17 hsp70 genes were identified through the genome of the large yellow croaker. These genes are divided into seven evolutionarily distinct groups according to a phylogenetic tree. The orthologs of these hsp70 genes were found in humans and zebrafish. The expression patterns of the hsp70 gene family in the large yellow croaker under cold and heat stress were studied by examining transcriptome data. Six out of 17 genes were significantly unregulated or downregulated after cold or heat stress. There were two genes significantly upregulated and two genes downregulated in the liver after cold treatment, while after heat treatment, five genes were significantly upregulated, and no genes were significantly downregulated. Three expression patterns were detected: strictly heat-inducible hsp70, constitutively expressed and moderately heat-inducible hsp70, and constitutively expressed and less stress-dependent hsp70 genes. All the findings will contribute to a better understanding of the biological function of hsp70s in defending against thermal challenges. MDPI 2018-11-29 /pmc/articles/PMC6316144/ /pubmed/30501106 http://dx.doi.org/10.3390/genes9120590 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Kaida
Xu, Hanxiang
Han, Zhiqiang
Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
title Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
title_full Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
title_fullStr Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
title_full_unstemmed Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
title_short Genome-Wide Identification of Hsp70 Genes in the Large Yellow Croaker (Larimichthys crocea) and Their Regulated Expression Under Cold and Heat Stress
title_sort genome-wide identification of hsp70 genes in the large yellow croaker (larimichthys crocea) and their regulated expression under cold and heat stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316144/
https://www.ncbi.nlm.nih.gov/pubmed/30501106
http://dx.doi.org/10.3390/genes9120590
work_keys_str_mv AT xukaida genomewideidentificationofhsp70genesinthelargeyellowcroakerlarimichthyscroceaandtheirregulatedexpressionundercoldandheatstress
AT xuhanxiang genomewideidentificationofhsp70genesinthelargeyellowcroakerlarimichthyscroceaandtheirregulatedexpressionundercoldandheatstress
AT hanzhiqiang genomewideidentificationofhsp70genesinthelargeyellowcroakerlarimichthyscroceaandtheirregulatedexpressionundercoldandheatstress