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Microdialysis-Assessed Adipose Tissue Metabolism, Circulating Cytokines and Outcome in Critical Illness

Microdialysis (MD) can provide continuous information about tissue composition. To assess in critically ill patients adipose tissue metabolic patterns, the relationships between metabolic patterns and blood cytokine concentration associations of adipose tissue energy metabolism and clinical outcome...

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Autores principales: Ilias, Ioannis, Apollonatou, Sofia, Nikitas, Nikitas, Theodorakopoulou, Maria, Vassiliou, Alice G, Kotanidou, Anastasia, Dimopoulou, Ioanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316198/
https://www.ncbi.nlm.nih.gov/pubmed/30301230
http://dx.doi.org/10.3390/metabo8040062
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author Ilias, Ioannis
Apollonatou, Sofia
Nikitas, Nikitas
Theodorakopoulou, Maria
Vassiliou, Alice G
Kotanidou, Anastasia
Dimopoulou, Ioanna
author_facet Ilias, Ioannis
Apollonatou, Sofia
Nikitas, Nikitas
Theodorakopoulou, Maria
Vassiliou, Alice G
Kotanidou, Anastasia
Dimopoulou, Ioanna
author_sort Ilias, Ioannis
collection PubMed
description Microdialysis (MD) can provide continuous information about tissue composition. To assess in critically ill patients adipose tissue metabolic patterns, the relationships between metabolic patterns and blood cytokine concentration associations of adipose tissue energy metabolism and clinical outcome we studied 203 mechanically ventilated general intensive care unit (ICU) patients. Upon ICU admission an MD catheter was inserted into the subcutaneous adipose tissue of the upper thigh to measure lactate (L), glucose, pyruvate (P), and glycerol. Serum concentrations of IL-10, IL-6, IL-8, and TNF-α were determined within 48 h from ICU admission. Mitochondrial dysfunction was defined as L/P ratio >30 and pyruvate ≥70 μmol/L, ischemia as L/P ratio >30 and pyruvate <70 μmol/L and no ischemia/no mitochondrial dysfunction (i.e., aerobic metabolism) was as L/P ratio ≤30. Metabolism was aerobic in 74% of patients. In 13% of patients there was biochemical evidence of ischemia and in 13% of patients of mitochondrial dysfunction. Mitochondrial dysfunction was associated with poor outcome. In conclusion, MD showed that about two thirds of critically ill patients have normal aerobic adipose tissue metabolism. Mitochondrial dysfunction was not common but was associated with poor outcome. Identifying subgroups of critically ill patients is crucial as different treatment strategies may improve survival.
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spelling pubmed-63161982019-01-10 Microdialysis-Assessed Adipose Tissue Metabolism, Circulating Cytokines and Outcome in Critical Illness Ilias, Ioannis Apollonatou, Sofia Nikitas, Nikitas Theodorakopoulou, Maria Vassiliou, Alice G Kotanidou, Anastasia Dimopoulou, Ioanna Metabolites Article Microdialysis (MD) can provide continuous information about tissue composition. To assess in critically ill patients adipose tissue metabolic patterns, the relationships between metabolic patterns and blood cytokine concentration associations of adipose tissue energy metabolism and clinical outcome we studied 203 mechanically ventilated general intensive care unit (ICU) patients. Upon ICU admission an MD catheter was inserted into the subcutaneous adipose tissue of the upper thigh to measure lactate (L), glucose, pyruvate (P), and glycerol. Serum concentrations of IL-10, IL-6, IL-8, and TNF-α were determined within 48 h from ICU admission. Mitochondrial dysfunction was defined as L/P ratio >30 and pyruvate ≥70 μmol/L, ischemia as L/P ratio >30 and pyruvate <70 μmol/L and no ischemia/no mitochondrial dysfunction (i.e., aerobic metabolism) was as L/P ratio ≤30. Metabolism was aerobic in 74% of patients. In 13% of patients there was biochemical evidence of ischemia and in 13% of patients of mitochondrial dysfunction. Mitochondrial dysfunction was associated with poor outcome. In conclusion, MD showed that about two thirds of critically ill patients have normal aerobic adipose tissue metabolism. Mitochondrial dysfunction was not common but was associated with poor outcome. Identifying subgroups of critically ill patients is crucial as different treatment strategies may improve survival. MDPI 2018-10-06 /pmc/articles/PMC6316198/ /pubmed/30301230 http://dx.doi.org/10.3390/metabo8040062 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ilias, Ioannis
Apollonatou, Sofia
Nikitas, Nikitas
Theodorakopoulou, Maria
Vassiliou, Alice G
Kotanidou, Anastasia
Dimopoulou, Ioanna
Microdialysis-Assessed Adipose Tissue Metabolism, Circulating Cytokines and Outcome in Critical Illness
title Microdialysis-Assessed Adipose Tissue Metabolism, Circulating Cytokines and Outcome in Critical Illness
title_full Microdialysis-Assessed Adipose Tissue Metabolism, Circulating Cytokines and Outcome in Critical Illness
title_fullStr Microdialysis-Assessed Adipose Tissue Metabolism, Circulating Cytokines and Outcome in Critical Illness
title_full_unstemmed Microdialysis-Assessed Adipose Tissue Metabolism, Circulating Cytokines and Outcome in Critical Illness
title_short Microdialysis-Assessed Adipose Tissue Metabolism, Circulating Cytokines and Outcome in Critical Illness
title_sort microdialysis-assessed adipose tissue metabolism, circulating cytokines and outcome in critical illness
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316198/
https://www.ncbi.nlm.nih.gov/pubmed/30301230
http://dx.doi.org/10.3390/metabo8040062
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