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Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity
Cereus jamacaru D.C. (mandacaru) is a cactus used as food and in the traditional medicine. In the present study, hydroalcoholic extract of C. jamacaru was evaluated for its chemical composition, antioxidant activity, cytotoxic and anti-cytotoxic effects in human lymphocytes and sarcoma 180 cells in...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316405/ https://www.ncbi.nlm.nih.gov/pubmed/30477180 http://dx.doi.org/10.3390/ph11040130 |
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author | Vencioneck Dutra, Jean Carlos Moisés Ferreira, Jean Costalonga Pereira, Paula Roberta Ben-Hur de Oliveira, Judá Vitorino Gervásio, Suiany Bernardes Xavier, Mirieli Mantovanelli da Mota, Mainã da Luz, Anny Carolyne Rodrigues Pretti, Irany Seibert França, Hildegardo Masrouah Jamal, Claudia Pimentel Batitucci, Maria do Carmo |
author_facet | Vencioneck Dutra, Jean Carlos Moisés Ferreira, Jean Costalonga Pereira, Paula Roberta Ben-Hur de Oliveira, Judá Vitorino Gervásio, Suiany Bernardes Xavier, Mirieli Mantovanelli da Mota, Mainã da Luz, Anny Carolyne Rodrigues Pretti, Irany Seibert França, Hildegardo Masrouah Jamal, Claudia Pimentel Batitucci, Maria do Carmo |
author_sort | Vencioneck Dutra, Jean Carlos |
collection | PubMed |
description | Cereus jamacaru D.C. (mandacaru) is a cactus used as food and in the traditional medicine. In the present study, hydroalcoholic extract of C. jamacaru was evaluated for its chemical composition, antioxidant activity, cytotoxic and anti-cytotoxic effects in human lymphocytes and sarcoma 180 cells in vitro by MTT assay and antitumoral, mutagenic and cytotoxic effects on mice sarcoma-induced in vivo. Phytochemical characterization showed positive reactions for coumarin, flavanol and tyramine and total flavonoid content of 0.51 µg/mL. C. jamacaru showed antioxidant activity following DPPH (EC(50) = 427.74 µg/mL), ABTS (EC(50) = 270.57 µg/mL) and Fe(2+) chelating ions assays (EC(50) = 41.18 µg/mL). C. jamacaru induced significant decrease of sarcoma 180 viability at 24 h and 48 h of treatment, did not induce cytotoxicity in human lymphocytes and inhibits the cytotoxicity of cisplatin in vitro. Following in vivo assays, C. jamacaru promoted tumor reduction (86.07% of tumor inhibition), without inducing mutagenic or cytotoxic damage on mice blood cells. We propose that phenolic and alkaloid compounds in the extract are related to antioxidant activity, increasing its ability in metal chelating activity and promoting anti-cytotoxic activity against cisplatin, as well as these compounds may act on the cell cycle of the tumor cells in vitro and in vivo, leading to anticancer effects and tumor reduction. |
format | Online Article Text |
id | pubmed-6316405 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63164052019-01-11 Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity Vencioneck Dutra, Jean Carlos Moisés Ferreira, Jean Costalonga Pereira, Paula Roberta Ben-Hur de Oliveira, Judá Vitorino Gervásio, Suiany Bernardes Xavier, Mirieli Mantovanelli da Mota, Mainã da Luz, Anny Carolyne Rodrigues Pretti, Irany Seibert França, Hildegardo Masrouah Jamal, Claudia Pimentel Batitucci, Maria do Carmo Pharmaceuticals (Basel) Article Cereus jamacaru D.C. (mandacaru) is a cactus used as food and in the traditional medicine. In the present study, hydroalcoholic extract of C. jamacaru was evaluated for its chemical composition, antioxidant activity, cytotoxic and anti-cytotoxic effects in human lymphocytes and sarcoma 180 cells in vitro by MTT assay and antitumoral, mutagenic and cytotoxic effects on mice sarcoma-induced in vivo. Phytochemical characterization showed positive reactions for coumarin, flavanol and tyramine and total flavonoid content of 0.51 µg/mL. C. jamacaru showed antioxidant activity following DPPH (EC(50) = 427.74 µg/mL), ABTS (EC(50) = 270.57 µg/mL) and Fe(2+) chelating ions assays (EC(50) = 41.18 µg/mL). C. jamacaru induced significant decrease of sarcoma 180 viability at 24 h and 48 h of treatment, did not induce cytotoxicity in human lymphocytes and inhibits the cytotoxicity of cisplatin in vitro. Following in vivo assays, C. jamacaru promoted tumor reduction (86.07% of tumor inhibition), without inducing mutagenic or cytotoxic damage on mice blood cells. We propose that phenolic and alkaloid compounds in the extract are related to antioxidant activity, increasing its ability in metal chelating activity and promoting anti-cytotoxic activity against cisplatin, as well as these compounds may act on the cell cycle of the tumor cells in vitro and in vivo, leading to anticancer effects and tumor reduction. MDPI 2018-11-23 /pmc/articles/PMC6316405/ /pubmed/30477180 http://dx.doi.org/10.3390/ph11040130 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vencioneck Dutra, Jean Carlos Moisés Ferreira, Jean Costalonga Pereira, Paula Roberta Ben-Hur de Oliveira, Judá Vitorino Gervásio, Suiany Bernardes Xavier, Mirieli Mantovanelli da Mota, Mainã da Luz, Anny Carolyne Rodrigues Pretti, Irany Seibert França, Hildegardo Masrouah Jamal, Claudia Pimentel Batitucci, Maria do Carmo Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity |
title | Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity |
title_full | Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity |
title_fullStr | Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity |
title_full_unstemmed | Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity |
title_short | Cereus jamacaru D.C. Hydroalcoholic Extract Promotes Anti-Cytotoxic and Antitumor Activity |
title_sort | cereus jamacaru d.c. hydroalcoholic extract promotes anti-cytotoxic and antitumor activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316405/ https://www.ncbi.nlm.nih.gov/pubmed/30477180 http://dx.doi.org/10.3390/ph11040130 |
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