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Effect of Surface Coating of Gold Nanoparticles on Cytotoxicity and Cell Cycle Progression
Gold nanoparticles (GNPs) are usually wrapped with biocompatible polymers in biomedical field, however, the effect of biocompatible polymers of gold nanoparticles on cellular responses are still not fully understood. In this study, GNPs with/without polymer wrapping were used as model probes for the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316730/ https://www.ncbi.nlm.nih.gov/pubmed/30562921 http://dx.doi.org/10.3390/nano8121063 |
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author | Li, Qian Huang, Chun Liu, Liwei Hu, Rui Qu, Junle |
author_facet | Li, Qian Huang, Chun Liu, Liwei Hu, Rui Qu, Junle |
author_sort | Li, Qian |
collection | PubMed |
description | Gold nanoparticles (GNPs) are usually wrapped with biocompatible polymers in biomedical field, however, the effect of biocompatible polymers of gold nanoparticles on cellular responses are still not fully understood. In this study, GNPs with/without polymer wrapping were used as model probes for the investigation of cytotoxicity and cell cycle progression. Our results show that the bovine serum albumin (BSA) coated GNPs (BSA-GNPs) had been transported into lysosomes after endocytosis. The lysosomal accumulation had then led to increased binding between kinesin 5 and microtubules, enhanced microtubule stabilization, and eventually induced G(2)/M arrest through the regulation of cadherin 1. In contrast, the bare GNPs experienced lysosomal escape, resulting in microtubule damage and G(0)/G(1) arrest through the regulation of proliferating cell nuclear antigen. Overall, our findings showed that both naked and BSA wrapped gold nanoparticles had cytotoxicity, however, they affected cell proliferation via different pathways. This will greatly help us to regulate cell responses for different biomedical applications. |
format | Online Article Text |
id | pubmed-6316730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63167302019-01-10 Effect of Surface Coating of Gold Nanoparticles on Cytotoxicity and Cell Cycle Progression Li, Qian Huang, Chun Liu, Liwei Hu, Rui Qu, Junle Nanomaterials (Basel) Article Gold nanoparticles (GNPs) are usually wrapped with biocompatible polymers in biomedical field, however, the effect of biocompatible polymers of gold nanoparticles on cellular responses are still not fully understood. In this study, GNPs with/without polymer wrapping were used as model probes for the investigation of cytotoxicity and cell cycle progression. Our results show that the bovine serum albumin (BSA) coated GNPs (BSA-GNPs) had been transported into lysosomes after endocytosis. The lysosomal accumulation had then led to increased binding between kinesin 5 and microtubules, enhanced microtubule stabilization, and eventually induced G(2)/M arrest through the regulation of cadherin 1. In contrast, the bare GNPs experienced lysosomal escape, resulting in microtubule damage and G(0)/G(1) arrest through the regulation of proliferating cell nuclear antigen. Overall, our findings showed that both naked and BSA wrapped gold nanoparticles had cytotoxicity, however, they affected cell proliferation via different pathways. This will greatly help us to regulate cell responses for different biomedical applications. MDPI 2018-12-17 /pmc/articles/PMC6316730/ /pubmed/30562921 http://dx.doi.org/10.3390/nano8121063 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Qian Huang, Chun Liu, Liwei Hu, Rui Qu, Junle Effect of Surface Coating of Gold Nanoparticles on Cytotoxicity and Cell Cycle Progression |
title | Effect of Surface Coating of Gold Nanoparticles on Cytotoxicity and Cell Cycle Progression |
title_full | Effect of Surface Coating of Gold Nanoparticles on Cytotoxicity and Cell Cycle Progression |
title_fullStr | Effect of Surface Coating of Gold Nanoparticles on Cytotoxicity and Cell Cycle Progression |
title_full_unstemmed | Effect of Surface Coating of Gold Nanoparticles on Cytotoxicity and Cell Cycle Progression |
title_short | Effect of Surface Coating of Gold Nanoparticles on Cytotoxicity and Cell Cycle Progression |
title_sort | effect of surface coating of gold nanoparticles on cytotoxicity and cell cycle progression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316730/ https://www.ncbi.nlm.nih.gov/pubmed/30562921 http://dx.doi.org/10.3390/nano8121063 |
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