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Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications
Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from th...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316778/ https://www.ncbi.nlm.nih.gov/pubmed/30544788 http://dx.doi.org/10.3390/md16120495 |
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author | Carvalho, Ana M. Marques, Alexandra P. Silva, Tiago H. Reis, Rui L. |
author_facet | Carvalho, Ana M. Marques, Alexandra P. Silva, Tiago H. Reis, Rui L. |
author_sort | Carvalho, Ana M. |
collection | PubMed |
description | Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 °C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications. |
format | Online Article Text |
id | pubmed-6316778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63167782019-01-10 Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications Carvalho, Ana M. Marques, Alexandra P. Silva, Tiago H. Reis, Rui L. Mar Drugs Article Collagen is one of the most widely used biomaterials, not only due its biocompatibility, biodegradability and weak antigenic potential, but also due to its role in the structure and function of tissues. Searching for alternative collagen sources, the aim of this study was to extract collagen from the skin of codfish, previously obtained as a by-product of fish industrial plants, and characterize it regarding its use as a biomaterial for biomedical application, according to American Society for Testing and Materials (ASTM) Guidelines. Collagen type I with a high degree of purity was obtained through acid-extraction, as confirmed by colorimetric assays, SDS-PAGE and amino acid composition. Thermal analysis revealed a denaturing temperature around 16 °C. Moreover, collagen showed a concentration-dependent effect in metabolism and on cell adhesion of lung fibroblast MRC-5 cells. In conclusion, this study shows that collagen can be obtained from marine-origin sources, while preserving its bioactivity, supporting its use in biomedical applications. MDPI 2018-12-08 /pmc/articles/PMC6316778/ /pubmed/30544788 http://dx.doi.org/10.3390/md16120495 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Carvalho, Ana M. Marques, Alexandra P. Silva, Tiago H. Reis, Rui L. Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications |
title | Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications |
title_full | Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications |
title_fullStr | Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications |
title_full_unstemmed | Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications |
title_short | Evaluation of the Potential of Collagen from Codfish Skin as a Biomaterial for Biomedical Applications |
title_sort | evaluation of the potential of collagen from codfish skin as a biomaterial for biomedical applications |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316778/ https://www.ncbi.nlm.nih.gov/pubmed/30544788 http://dx.doi.org/10.3390/md16120495 |
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