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Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection
The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition o...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316885/ https://www.ncbi.nlm.nih.gov/pubmed/30332738 http://dx.doi.org/10.3390/bios8040091 |
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author | Milano, Francesco Giotta, Livia Chirizzi, Daniela Papazoglou, Simos Kryou, Christina De Bartolomeo, Annarita De Leo, Vincenzo Guascito, Maria Rachele Zergioti, Ioanna |
author_facet | Milano, Francesco Giotta, Livia Chirizzi, Daniela Papazoglou, Simos Kryou, Christina De Bartolomeo, Annarita De Leo, Vincenzo Guascito, Maria Rachele Zergioti, Ioanna |
author_sort | Milano, Francesco |
collection | PubMed |
description | The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition of various materials (even labile materials like enzymes) that results in intimate and stable contact with target surface. In this work, Pt, Au, and glassy carbon screen printed electrodes (SPEs) treated by LIFT with phosphate buffer have been characterized by scanning electron microscopy and atomic force microscopy to reveal a flattening effect of all surfaces. The electrochemical characterization by cyclic voltammetry shows significant differences depending on the electrode material. The electroactivity of Au is reduced while that of glassy carbon and Pt is greatly enhanced. In particular, the electrochemical behavior of a phosphate LIFT treated Pt showed a marked enrichment of hydrogen adsorbed layer, suggesting an elevated electrocatalytic activity towards glucose oxidation. When Pt electrodes modified in this way were used as an effective glucose sensor, a 1–10 mM linear response and a 10 µM detection limit were obtained. A possible role of phosphate that was securely immobilized on a Pt surface, as evidenced by XPS analysis, enhancing the glucose electrooxidation is discussed. |
format | Online Article Text |
id | pubmed-6316885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63168852019-01-09 Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection Milano, Francesco Giotta, Livia Chirizzi, Daniela Papazoglou, Simos Kryou, Christina De Bartolomeo, Annarita De Leo, Vincenzo Guascito, Maria Rachele Zergioti, Ioanna Biosensors (Basel) Article The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition of various materials (even labile materials like enzymes) that results in intimate and stable contact with target surface. In this work, Pt, Au, and glassy carbon screen printed electrodes (SPEs) treated by LIFT with phosphate buffer have been characterized by scanning electron microscopy and atomic force microscopy to reveal a flattening effect of all surfaces. The electrochemical characterization by cyclic voltammetry shows significant differences depending on the electrode material. The electroactivity of Au is reduced while that of glassy carbon and Pt is greatly enhanced. In particular, the electrochemical behavior of a phosphate LIFT treated Pt showed a marked enrichment of hydrogen adsorbed layer, suggesting an elevated electrocatalytic activity towards glucose oxidation. When Pt electrodes modified in this way were used as an effective glucose sensor, a 1–10 mM linear response and a 10 µM detection limit were obtained. A possible role of phosphate that was securely immobilized on a Pt surface, as evidenced by XPS analysis, enhancing the glucose electrooxidation is discussed. MDPI 2018-10-16 /pmc/articles/PMC6316885/ /pubmed/30332738 http://dx.doi.org/10.3390/bios8040091 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Milano, Francesco Giotta, Livia Chirizzi, Daniela Papazoglou, Simos Kryou, Christina De Bartolomeo, Annarita De Leo, Vincenzo Guascito, Maria Rachele Zergioti, Ioanna Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
title | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
title_full | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
title_fullStr | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
title_full_unstemmed | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
title_short | Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection |
title_sort | phosphate modified screen printed electrodes by lift treatment for glucose detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316885/ https://www.ncbi.nlm.nih.gov/pubmed/30332738 http://dx.doi.org/10.3390/bios8040091 |
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