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Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection

The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition o...

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Autores principales: Milano, Francesco, Giotta, Livia, Chirizzi, Daniela, Papazoglou, Simos, Kryou, Christina, De Bartolomeo, Annarita, De Leo, Vincenzo, Guascito, Maria Rachele, Zergioti, Ioanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316885/
https://www.ncbi.nlm.nih.gov/pubmed/30332738
http://dx.doi.org/10.3390/bios8040091
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author Milano, Francesco
Giotta, Livia
Chirizzi, Daniela
Papazoglou, Simos
Kryou, Christina
De Bartolomeo, Annarita
De Leo, Vincenzo
Guascito, Maria Rachele
Zergioti, Ioanna
author_facet Milano, Francesco
Giotta, Livia
Chirizzi, Daniela
Papazoglou, Simos
Kryou, Christina
De Bartolomeo, Annarita
De Leo, Vincenzo
Guascito, Maria Rachele
Zergioti, Ioanna
author_sort Milano, Francesco
collection PubMed
description The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition of various materials (even labile materials like enzymes) that results in intimate and stable contact with target surface. In this work, Pt, Au, and glassy carbon screen printed electrodes (SPEs) treated by LIFT with phosphate buffer have been characterized by scanning electron microscopy and atomic force microscopy to reveal a flattening effect of all surfaces. The electrochemical characterization by cyclic voltammetry shows significant differences depending on the electrode material. The electroactivity of Au is reduced while that of glassy carbon and Pt is greatly enhanced. In particular, the electrochemical behavior of a phosphate LIFT treated Pt showed a marked enrichment of hydrogen adsorbed layer, suggesting an elevated electrocatalytic activity towards glucose oxidation. When Pt electrodes modified in this way were used as an effective glucose sensor, a 1–10 mM linear response and a 10 µM detection limit were obtained. A possible role of phosphate that was securely immobilized on a Pt surface, as evidenced by XPS analysis, enhancing the glucose electrooxidation is discussed.
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spelling pubmed-63168852019-01-09 Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection Milano, Francesco Giotta, Livia Chirizzi, Daniela Papazoglou, Simos Kryou, Christina De Bartolomeo, Annarita De Leo, Vincenzo Guascito, Maria Rachele Zergioti, Ioanna Biosensors (Basel) Article The design of new materials as active layers is important for electrochemical sensor and biosensor development. Among the techniques for the modification and functionalization of electrodes, the laser induced forward transfer (LIFT) has emerged as a powerful physisorption method for the deposition of various materials (even labile materials like enzymes) that results in intimate and stable contact with target surface. In this work, Pt, Au, and glassy carbon screen printed electrodes (SPEs) treated by LIFT with phosphate buffer have been characterized by scanning electron microscopy and atomic force microscopy to reveal a flattening effect of all surfaces. The electrochemical characterization by cyclic voltammetry shows significant differences depending on the electrode material. The electroactivity of Au is reduced while that of glassy carbon and Pt is greatly enhanced. In particular, the electrochemical behavior of a phosphate LIFT treated Pt showed a marked enrichment of hydrogen adsorbed layer, suggesting an elevated electrocatalytic activity towards glucose oxidation. When Pt electrodes modified in this way were used as an effective glucose sensor, a 1–10 mM linear response and a 10 µM detection limit were obtained. A possible role of phosphate that was securely immobilized on a Pt surface, as evidenced by XPS analysis, enhancing the glucose electrooxidation is discussed. MDPI 2018-10-16 /pmc/articles/PMC6316885/ /pubmed/30332738 http://dx.doi.org/10.3390/bios8040091 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Milano, Francesco
Giotta, Livia
Chirizzi, Daniela
Papazoglou, Simos
Kryou, Christina
De Bartolomeo, Annarita
De Leo, Vincenzo
Guascito, Maria Rachele
Zergioti, Ioanna
Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection
title Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection
title_full Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection
title_fullStr Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection
title_full_unstemmed Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection
title_short Phosphate Modified Screen Printed Electrodes by LIFT Treatment for Glucose Detection
title_sort phosphate modified screen printed electrodes by lift treatment for glucose detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6316885/
https://www.ncbi.nlm.nih.gov/pubmed/30332738
http://dx.doi.org/10.3390/bios8040091
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