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HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation
BACKGROUND: Previous studies have demonstrated that the expression of homeobox8 (HOXB8) is higher in colorectal cancer (CRC) tissues than in normal tissues; however, the precise role of HOXB8 in human CRC cells remains to be elucidated. METHODS: We generated lentiviral constructs to overexpress and...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317211/ https://www.ncbi.nlm.nih.gov/pubmed/30622439 http://dx.doi.org/10.1186/s12935-018-0717-6 |
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author | Wang, Tingting Lin, Feiyan Sun, Xuecheng Jiang, Lei Mao, Ruibo Zhou, Shenyue Shang, Wenjing Bi, Ruichun Lu, Fengying Li, Shaotang |
author_facet | Wang, Tingting Lin, Feiyan Sun, Xuecheng Jiang, Lei Mao, Ruibo Zhou, Shenyue Shang, Wenjing Bi, Ruichun Lu, Fengying Li, Shaotang |
author_sort | Wang, Tingting |
collection | PubMed |
description | BACKGROUND: Previous studies have demonstrated that the expression of homeobox8 (HOXB8) is higher in colorectal cancer (CRC) tissues than in normal tissues; however, the precise role of HOXB8 in human CRC cells remains to be elucidated. METHODS: We generated lentiviral constructs to overexpress and silence HOXB8 in CRC cell lines, and examined their biological functions through MTT, wound healing, colony and transwell, expression of signal transducer and activator of transcription 3 (STAT3) and epithelial–mesenchymal transition (EMT) related factors through western-blot. RESULTS: HOXB8 knockdown inhibited cellular proliferation and invasion in vitro as well as carcinogenesis and metastasis in vivo. HOXB8 also induced EMT, which is characterized by the down-regulation of E-cadherin and the up-regulation of Vimentin, N-cadherin, Twist, Zeb1 and Zeb2. Moreover, HOXB8 activated STAT3, which is known to play an oncogenic role in diverse human malignancies. CONCLUSIONS: Our results indicate that HOXB8 may be an independent prognostic factor in CRC. Therefore, deserved a deeper research. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12935-018-0717-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6317211 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63172112019-01-08 HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation Wang, Tingting Lin, Feiyan Sun, Xuecheng Jiang, Lei Mao, Ruibo Zhou, Shenyue Shang, Wenjing Bi, Ruichun Lu, Fengying Li, Shaotang Cancer Cell Int Primary Research BACKGROUND: Previous studies have demonstrated that the expression of homeobox8 (HOXB8) is higher in colorectal cancer (CRC) tissues than in normal tissues; however, the precise role of HOXB8 in human CRC cells remains to be elucidated. METHODS: We generated lentiviral constructs to overexpress and silence HOXB8 in CRC cell lines, and examined their biological functions through MTT, wound healing, colony and transwell, expression of signal transducer and activator of transcription 3 (STAT3) and epithelial–mesenchymal transition (EMT) related factors through western-blot. RESULTS: HOXB8 knockdown inhibited cellular proliferation and invasion in vitro as well as carcinogenesis and metastasis in vivo. HOXB8 also induced EMT, which is characterized by the down-regulation of E-cadherin and the up-regulation of Vimentin, N-cadherin, Twist, Zeb1 and Zeb2. Moreover, HOXB8 activated STAT3, which is known to play an oncogenic role in diverse human malignancies. CONCLUSIONS: Our results indicate that HOXB8 may be an independent prognostic factor in CRC. Therefore, deserved a deeper research. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12935-018-0717-6) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-03 /pmc/articles/PMC6317211/ /pubmed/30622439 http://dx.doi.org/10.1186/s12935-018-0717-6 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Primary Research Wang, Tingting Lin, Feiyan Sun, Xuecheng Jiang, Lei Mao, Ruibo Zhou, Shenyue Shang, Wenjing Bi, Ruichun Lu, Fengying Li, Shaotang HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation |
title | HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation |
title_full | HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation |
title_fullStr | HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation |
title_full_unstemmed | HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation |
title_short | HOXB8 enhances the proliferation and metastasis of colorectal cancer cells by promoting EMT via STAT3 activation |
title_sort | hoxb8 enhances the proliferation and metastasis of colorectal cancer cells by promoting emt via stat3 activation |
topic | Primary Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317211/ https://www.ncbi.nlm.nih.gov/pubmed/30622439 http://dx.doi.org/10.1186/s12935-018-0717-6 |
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