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Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family

DNA methylation is essential for gene regulation, transposon silencing, and imprinting. Although the generation of specific DNA methylation patterns is critical for these processes, how methylation is regulated at individual loci remains unclear. Here we show that a family of four putative chromatin...

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Autores principales: Zhou, Ming, Palanca, Ana Marie S., Law, Julie A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317521/
https://www.ncbi.nlm.nih.gov/pubmed/29736015
http://dx.doi.org/10.1038/s41588-018-0115-y
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author Zhou, Ming
Palanca, Ana Marie S.
Law, Julie A.
author_facet Zhou, Ming
Palanca, Ana Marie S.
Law, Julie A.
author_sort Zhou, Ming
collection PubMed
description DNA methylation is essential for gene regulation, transposon silencing, and imprinting. Although the generation of specific DNA methylation patterns is critical for these processes, how methylation is regulated at individual loci remains unclear. Here we show that a family of four putative chromatin remodeling factors, CLASSY (CLSY) 1–4, are required for both locus-specific and global regulation of DNA methylation in Arabidopsis. Mechanistically, these factors act in connection with RNA polymerase-IV (Pol-IV) to control the production of 24-nucleotide small interfering RNAs (24nt-siRNAs), which guide DNA methylation. Individually, the CLSYs regulate Pol-IV-chromatin association and 24nt-siRNA production at thousands of distinct loci, and together, they regulate essentially all 24nt-siRNAs. Depending on the CLSYs involved, this regulation relies on different repressive chromatin modifications to facilitate locus-specific control of DNA methylation. Given the conservation between methylation systems in plants and mammals, analogous pathways likely operate in a broad range of organisms.
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spelling pubmed-63175212019-01-03 Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family Zhou, Ming Palanca, Ana Marie S. Law, Julie A. Nat Genet Article DNA methylation is essential for gene regulation, transposon silencing, and imprinting. Although the generation of specific DNA methylation patterns is critical for these processes, how methylation is regulated at individual loci remains unclear. Here we show that a family of four putative chromatin remodeling factors, CLASSY (CLSY) 1–4, are required for both locus-specific and global regulation of DNA methylation in Arabidopsis. Mechanistically, these factors act in connection with RNA polymerase-IV (Pol-IV) to control the production of 24-nucleotide small interfering RNAs (24nt-siRNAs), which guide DNA methylation. Individually, the CLSYs regulate Pol-IV-chromatin association and 24nt-siRNA production at thousands of distinct loci, and together, they regulate essentially all 24nt-siRNAs. Depending on the CLSYs involved, this regulation relies on different repressive chromatin modifications to facilitate locus-specific control of DNA methylation. Given the conservation between methylation systems in plants and mammals, analogous pathways likely operate in a broad range of organisms. 2018-05-07 2018-06 /pmc/articles/PMC6317521/ /pubmed/29736015 http://dx.doi.org/10.1038/s41588-018-0115-y Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Zhou, Ming
Palanca, Ana Marie S.
Law, Julie A.
Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
title Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
title_full Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
title_fullStr Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
title_full_unstemmed Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
title_short Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
title_sort locus-specific control of the de novo dna methylation pathway in arabidopsis by the classy family
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317521/
https://www.ncbi.nlm.nih.gov/pubmed/29736015
http://dx.doi.org/10.1038/s41588-018-0115-y
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