Cargando…
Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family
DNA methylation is essential for gene regulation, transposon silencing, and imprinting. Although the generation of specific DNA methylation patterns is critical for these processes, how methylation is regulated at individual loci remains unclear. Here we show that a family of four putative chromatin...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317521/ https://www.ncbi.nlm.nih.gov/pubmed/29736015 http://dx.doi.org/10.1038/s41588-018-0115-y |
_version_ | 1783384754911969280 |
---|---|
author | Zhou, Ming Palanca, Ana Marie S. Law, Julie A. |
author_facet | Zhou, Ming Palanca, Ana Marie S. Law, Julie A. |
author_sort | Zhou, Ming |
collection | PubMed |
description | DNA methylation is essential for gene regulation, transposon silencing, and imprinting. Although the generation of specific DNA methylation patterns is critical for these processes, how methylation is regulated at individual loci remains unclear. Here we show that a family of four putative chromatin remodeling factors, CLASSY (CLSY) 1–4, are required for both locus-specific and global regulation of DNA methylation in Arabidopsis. Mechanistically, these factors act in connection with RNA polymerase-IV (Pol-IV) to control the production of 24-nucleotide small interfering RNAs (24nt-siRNAs), which guide DNA methylation. Individually, the CLSYs regulate Pol-IV-chromatin association and 24nt-siRNA production at thousands of distinct loci, and together, they regulate essentially all 24nt-siRNAs. Depending on the CLSYs involved, this regulation relies on different repressive chromatin modifications to facilitate locus-specific control of DNA methylation. Given the conservation between methylation systems in plants and mammals, analogous pathways likely operate in a broad range of organisms. |
format | Online Article Text |
id | pubmed-6317521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-63175212019-01-03 Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family Zhou, Ming Palanca, Ana Marie S. Law, Julie A. Nat Genet Article DNA methylation is essential for gene regulation, transposon silencing, and imprinting. Although the generation of specific DNA methylation patterns is critical for these processes, how methylation is regulated at individual loci remains unclear. Here we show that a family of four putative chromatin remodeling factors, CLASSY (CLSY) 1–4, are required for both locus-specific and global regulation of DNA methylation in Arabidopsis. Mechanistically, these factors act in connection with RNA polymerase-IV (Pol-IV) to control the production of 24-nucleotide small interfering RNAs (24nt-siRNAs), which guide DNA methylation. Individually, the CLSYs regulate Pol-IV-chromatin association and 24nt-siRNA production at thousands of distinct loci, and together, they regulate essentially all 24nt-siRNAs. Depending on the CLSYs involved, this regulation relies on different repressive chromatin modifications to facilitate locus-specific control of DNA methylation. Given the conservation between methylation systems in plants and mammals, analogous pathways likely operate in a broad range of organisms. 2018-05-07 2018-06 /pmc/articles/PMC6317521/ /pubmed/29736015 http://dx.doi.org/10.1038/s41588-018-0115-y Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Zhou, Ming Palanca, Ana Marie S. Law, Julie A. Locus-specific control of the de novo DNA methylation pathway in Arabidopsis by the CLASSY family |
title | Locus-specific control of the de novo DNA
methylation pathway in Arabidopsis by the CLASSY
family |
title_full | Locus-specific control of the de novo DNA
methylation pathway in Arabidopsis by the CLASSY
family |
title_fullStr | Locus-specific control of the de novo DNA
methylation pathway in Arabidopsis by the CLASSY
family |
title_full_unstemmed | Locus-specific control of the de novo DNA
methylation pathway in Arabidopsis by the CLASSY
family |
title_short | Locus-specific control of the de novo DNA
methylation pathway in Arabidopsis by the CLASSY
family |
title_sort | locus-specific control of the de novo dna
methylation pathway in arabidopsis by the classy
family |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317521/ https://www.ncbi.nlm.nih.gov/pubmed/29736015 http://dx.doi.org/10.1038/s41588-018-0115-y |
work_keys_str_mv | AT zhouming locusspecificcontrolofthedenovodnamethylationpathwayinarabidopsisbytheclassyfamily AT palancaanamaries locusspecificcontrolofthedenovodnamethylationpathwayinarabidopsisbytheclassyfamily AT lawjuliea locusspecificcontrolofthedenovodnamethylationpathwayinarabidopsisbytheclassyfamily |