Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry

The ability to design detergents that are suitable for protein analysis by mass spectrometry (MS) represents an on-going challenge in the field of native MS. Desirable detergent characteristics include charge-reducing properties and low gas-phase stabilities of complexes formed with proteins. In thi...

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Detalles Bibliográficos
Autores principales: Urner, Leonhard H., Maier, Yasmine B., Haag, Rainer, Pagel, Kevin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2018
Materias:
Focus: Honoring Carol V. Robinson's Election to the National Academy of Sciences: Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318253/
https://www.ncbi.nlm.nih.gov/pubmed/30276626
http://dx.doi.org/10.1007/s13361-018-2063-2
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author Urner, Leonhard H.
Maier, Yasmine B.
Haag, Rainer
Pagel, Kevin
author_facet Urner, Leonhard H.
Maier, Yasmine B.
Haag, Rainer
Pagel, Kevin
author_sort Urner, Leonhard H.
collection PubMed
description The ability to design detergents that are suitable for protein analysis by mass spectrometry (MS) represents an on-going challenge in the field of native MS. Desirable detergent characteristics include charge-reducing properties and low gas-phase stabilities of complexes formed with proteins. In this work, the gas-phase properties of oligoglycerol detergents (OGDs) are optimized by fine tuning of their molecular structure. Furthermore, a tandem mass spectrometry (MS/MS) approach is presented that estimates the gas-phase properties of detergents simply by studying the dissociation behaviour of protein-detergent complexes (PDCs) formed with the soluble protein β-lactoglobulin (BLG). [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13361-018-2063-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-63182532019-01-14 Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry Urner, Leonhard H. Maier, Yasmine B. Haag, Rainer Pagel, Kevin J Am Soc Mass Spectrom Focus: Honoring Carol V. Robinson's Election to the National Academy of Sciences: Research Article The ability to design detergents that are suitable for protein analysis by mass spectrometry (MS) represents an on-going challenge in the field of native MS. Desirable detergent characteristics include charge-reducing properties and low gas-phase stabilities of complexes formed with proteins. In this work, the gas-phase properties of oligoglycerol detergents (OGDs) are optimized by fine tuning of their molecular structure. Furthermore, a tandem mass spectrometry (MS/MS) approach is presented that estimates the gas-phase properties of detergents simply by studying the dissociation behaviour of protein-detergent complexes (PDCs) formed with the soluble protein β-lactoglobulin (BLG). [Figure: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s13361-018-2063-2) contains supplementary material, which is available to authorized users. Springer US 2018-10-01 2019 /pmc/articles/PMC6318253/ /pubmed/30276626 http://dx.doi.org/10.1007/s13361-018-2063-2 Text en © The Author(s) 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Focus: Honoring Carol V. Robinson's Election to the National Academy of Sciences: Research Article
Urner, Leonhard H.
Maier, Yasmine B.
Haag, Rainer
Pagel, Kevin
Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry
title Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry
title_full Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry
title_fullStr Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry
title_full_unstemmed Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry
title_short Exploring the Potential of Dendritic Oligoglycerol Detergents for Protein Mass Spectrometry
title_sort exploring the potential of dendritic oligoglycerol detergents for protein mass spectrometry
topic Focus: Honoring Carol V. Robinson's Election to the National Academy of Sciences: Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318253/
https://www.ncbi.nlm.nih.gov/pubmed/30276626
http://dx.doi.org/10.1007/s13361-018-2063-2
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