Cargando…
Prevalence of Epstein–Barr Virus Genotypes in Pakistani Lymphoma Patients
The Epstein-Barr virus (EBV) is a herpesvirus infecting more than 90% of the human population. The tropism of EBV for B lymphocytes is evidenced in its association with many lymphoproliferative disorders. Different types of EBV (EBV-1 and EBV-2), classified on the basis of EBV nuclear antigen-2 (EBN...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
West Asia Organization for Cancer Prevention
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318387/ https://www.ncbi.nlm.nih.gov/pubmed/30486603 http://dx.doi.org/10.31557/APJCP.2018.19.11.3153 |
_version_ | 1783384865817755648 |
---|---|
author | Salahuddin, Sadia Khan, Jabbar Azhar, Joharia Whitehurst, Christopher B. Qadri, Ishtiaq Shackelford, Julia Pagano, Joseph S. Muhammad, Dost Richards, Kristy L. |
author_facet | Salahuddin, Sadia Khan, Jabbar Azhar, Joharia Whitehurst, Christopher B. Qadri, Ishtiaq Shackelford, Julia Pagano, Joseph S. Muhammad, Dost Richards, Kristy L. |
author_sort | Salahuddin, Sadia |
collection | PubMed |
description | The Epstein-Barr virus (EBV) is a herpesvirus infecting more than 90% of the human population. The tropism of EBV for B lymphocytes is evidenced in its association with many lymphoproliferative disorders. Different types of EBV (EBV-1 and EBV-2), classified on the basis of EBV nuclear antigen-2 (EBNA-2) genotyping, have been reported in benign and malignant pathologies, but there is almost no information about their frequency in the Pakistani population. The aim of this study was to determine the frequency and distribution of EBNA-2-based EBV genotypes in lymphoma patients. Genomic DNA was extracted from formalin-fixed paraffin embedded (FFPE) tissue samples obtained from 73 EBV-DNA-positive lymphoma patients. The β-globin gene was amplified to assess the presence and quality of cellular DNA from all samples. EBER-1 DNA was detected by PCR to confirm EBV presence in tissue samples. EBNA-1 mRNA relative quantification done by quantitative PCR substantiated EBNA-1 mRNA overexpression in 43.8% of EBV-positive cases in comparison to EBV-positive control cell line. EBNA-2 genotyping was done by nested PCR. Among typable samples, EBV-1 was found in 90.7% of samples while EBV-2 was present in 9.3% cases. These results show that EBV-1 was the most prevalent type in the lymphoma population of Pakistan. This epidemiology of EBV in Pakistani lymphoma patients represents an important first step in using EBV for prognosis and monitoring treatment response. |
format | Online Article Text |
id | pubmed-6318387 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | West Asia Organization for Cancer Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-63183872019-01-14 Prevalence of Epstein–Barr Virus Genotypes in Pakistani Lymphoma Patients Salahuddin, Sadia Khan, Jabbar Azhar, Joharia Whitehurst, Christopher B. Qadri, Ishtiaq Shackelford, Julia Pagano, Joseph S. Muhammad, Dost Richards, Kristy L. Asian Pac J Cancer Prev Research Article The Epstein-Barr virus (EBV) is a herpesvirus infecting more than 90% of the human population. The tropism of EBV for B lymphocytes is evidenced in its association with many lymphoproliferative disorders. Different types of EBV (EBV-1 and EBV-2), classified on the basis of EBV nuclear antigen-2 (EBNA-2) genotyping, have been reported in benign and malignant pathologies, but there is almost no information about their frequency in the Pakistani population. The aim of this study was to determine the frequency and distribution of EBNA-2-based EBV genotypes in lymphoma patients. Genomic DNA was extracted from formalin-fixed paraffin embedded (FFPE) tissue samples obtained from 73 EBV-DNA-positive lymphoma patients. The β-globin gene was amplified to assess the presence and quality of cellular DNA from all samples. EBER-1 DNA was detected by PCR to confirm EBV presence in tissue samples. EBNA-1 mRNA relative quantification done by quantitative PCR substantiated EBNA-1 mRNA overexpression in 43.8% of EBV-positive cases in comparison to EBV-positive control cell line. EBNA-2 genotyping was done by nested PCR. Among typable samples, EBV-1 was found in 90.7% of samples while EBV-2 was present in 9.3% cases. These results show that EBV-1 was the most prevalent type in the lymphoma population of Pakistan. This epidemiology of EBV in Pakistani lymphoma patients represents an important first step in using EBV for prognosis and monitoring treatment response. West Asia Organization for Cancer Prevention 2018 /pmc/articles/PMC6318387/ /pubmed/30486603 http://dx.doi.org/10.31557/APJCP.2018.19.11.3153 Text en Copyright: © Asian Pacific Journal of Cancer Prevention http://creativecommons.org/licenses/BY-SA/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License |
spellingShingle | Research Article Salahuddin, Sadia Khan, Jabbar Azhar, Joharia Whitehurst, Christopher B. Qadri, Ishtiaq Shackelford, Julia Pagano, Joseph S. Muhammad, Dost Richards, Kristy L. Prevalence of Epstein–Barr Virus Genotypes in Pakistani Lymphoma Patients |
title | Prevalence of Epstein–Barr Virus Genotypes in Pakistani Lymphoma Patients |
title_full | Prevalence of Epstein–Barr Virus Genotypes in Pakistani Lymphoma Patients |
title_fullStr | Prevalence of Epstein–Barr Virus Genotypes in Pakistani Lymphoma Patients |
title_full_unstemmed | Prevalence of Epstein–Barr Virus Genotypes in Pakistani Lymphoma Patients |
title_short | Prevalence of Epstein–Barr Virus Genotypes in Pakistani Lymphoma Patients |
title_sort | prevalence of epstein–barr virus genotypes in pakistani lymphoma patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318387/ https://www.ncbi.nlm.nih.gov/pubmed/30486603 http://dx.doi.org/10.31557/APJCP.2018.19.11.3153 |
work_keys_str_mv | AT salahuddinsadia prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients AT khanjabbar prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients AT azharjoharia prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients AT whitehurstchristopherb prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients AT qadriishtiaq prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients AT shackelfordjulia prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients AT paganojosephs prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients AT muhammaddost prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients AT richardskristyl prevalenceofepsteinbarrvirusgenotypesinpakistanilymphomapatients |