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Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum

BACKGROUND: Raw-starch-digesting glucoamylases (RSDGs) from filamentous fungi have great commercial values in starch processing; however, the regulatory mechanisms associated with their production in filamentous fungi remain unknown. Penicillium oxalicum HP7-1 isolated by our laboratory secretes RSD...

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Autores principales: Zhang, Mei-Yuan, Zhao, Shuai, Ning, Yuan-Ni, Fu, Li-Hao, Li, Cheng-Xi, Wang, Qi, You, Ran, Wang, Chen-Ying, Xu, Han-Nan, Luo, Xue-Mei, Feng, Jia-Xun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318894/
https://www.ncbi.nlm.nih.gov/pubmed/30622649
http://dx.doi.org/10.1186/s13068-018-1345-z
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author Zhang, Mei-Yuan
Zhao, Shuai
Ning, Yuan-Ni
Fu, Li-Hao
Li, Cheng-Xi
Wang, Qi
You, Ran
Wang, Chen-Ying
Xu, Han-Nan
Luo, Xue-Mei
Feng, Jia-Xun
author_facet Zhang, Mei-Yuan
Zhao, Shuai
Ning, Yuan-Ni
Fu, Li-Hao
Li, Cheng-Xi
Wang, Qi
You, Ran
Wang, Chen-Ying
Xu, Han-Nan
Luo, Xue-Mei
Feng, Jia-Xun
author_sort Zhang, Mei-Yuan
collection PubMed
description BACKGROUND: Raw-starch-digesting glucoamylases (RSDGs) from filamentous fungi have great commercial values in starch processing; however, the regulatory mechanisms associated with their production in filamentous fungi remain unknown. Penicillium oxalicum HP7-1 isolated by our laboratory secretes RSDG with suitable properties but at low production levels. Here, we screened and identified novel regulators of RSDG gene expression in P. oxalicum through transcriptional profiling and genetic analyses. RESULTS: Penicillium oxalicum HP7-1 transcriptomes in the presence of glucose and starch, respectively, used as the sole carbon source were comparatively analyzed, resulting in screening of 23 candidate genes regulating the expression of RSDG genes. Following deletion of 15 of the candidate genes in the parental P. oxalicum strain ∆PoxKu70, enzymatic assays revealed five mutants exhibiting significant reduction in the production of raw-starch-digesting enzymes (RSDEs). The deleted genes (POX01907, POX03446, POX06509, POX07078, and POX09752), were the first report to regulate RSDE production of P. oxalicum. Further analysis revealed that ∆POX01907 lost the most RSDE production (83.4%), and that POX01907 regulated the expression of major amylase genes, including the RSDG gene POX01356/PoxGA15A, a glucoamylase gene POX02412, and the α-amylase gene POX09352/Amy13A, during the late-stage growth of P. oxalicum. CONCLUSION: Our results revealed a novel essential regulatory gene POX01907 encoding a transcription factor in controlling the production of RSDE, regulating the expression of an important RSDG gene POX01356/PoxGA15A, in P. oxalicum. These results provide insight into the regulatory mechanism of fungal amylolytic enzyme production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1345-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-63188942019-01-08 Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum Zhang, Mei-Yuan Zhao, Shuai Ning, Yuan-Ni Fu, Li-Hao Li, Cheng-Xi Wang, Qi You, Ran Wang, Chen-Ying Xu, Han-Nan Luo, Xue-Mei Feng, Jia-Xun Biotechnol Biofuels Research BACKGROUND: Raw-starch-digesting glucoamylases (RSDGs) from filamentous fungi have great commercial values in starch processing; however, the regulatory mechanisms associated with their production in filamentous fungi remain unknown. Penicillium oxalicum HP7-1 isolated by our laboratory secretes RSDG with suitable properties but at low production levels. Here, we screened and identified novel regulators of RSDG gene expression in P. oxalicum through transcriptional profiling and genetic analyses. RESULTS: Penicillium oxalicum HP7-1 transcriptomes in the presence of glucose and starch, respectively, used as the sole carbon source were comparatively analyzed, resulting in screening of 23 candidate genes regulating the expression of RSDG genes. Following deletion of 15 of the candidate genes in the parental P. oxalicum strain ∆PoxKu70, enzymatic assays revealed five mutants exhibiting significant reduction in the production of raw-starch-digesting enzymes (RSDEs). The deleted genes (POX01907, POX03446, POX06509, POX07078, and POX09752), were the first report to regulate RSDE production of P. oxalicum. Further analysis revealed that ∆POX01907 lost the most RSDE production (83.4%), and that POX01907 regulated the expression of major amylase genes, including the RSDG gene POX01356/PoxGA15A, a glucoamylase gene POX02412, and the α-amylase gene POX09352/Amy13A, during the late-stage growth of P. oxalicum. CONCLUSION: Our results revealed a novel essential regulatory gene POX01907 encoding a transcription factor in controlling the production of RSDE, regulating the expression of an important RSDG gene POX01356/PoxGA15A, in P. oxalicum. These results provide insight into the regulatory mechanism of fungal amylolytic enzyme production. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13068-018-1345-z) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-04 /pmc/articles/PMC6318894/ /pubmed/30622649 http://dx.doi.org/10.1186/s13068-018-1345-z Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Mei-Yuan
Zhao, Shuai
Ning, Yuan-Ni
Fu, Li-Hao
Li, Cheng-Xi
Wang, Qi
You, Ran
Wang, Chen-Ying
Xu, Han-Nan
Luo, Xue-Mei
Feng, Jia-Xun
Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum
title Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum
title_full Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum
title_fullStr Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum
title_full_unstemmed Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum
title_short Identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in Penicillium oxalicum
title_sort identification of an essential regulator controlling the production of raw-starch-digesting glucoamylase in penicillium oxalicum
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318894/
https://www.ncbi.nlm.nih.gov/pubmed/30622649
http://dx.doi.org/10.1186/s13068-018-1345-z
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