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Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep

Fasciolosis is a parasitic disease of medical and economic importance. This retrospective study was conducted on 110 Fasciola flukes collected from livers of 14 infected Naimi sheep slaughtered at Riyadh abattoir in Saudi Arabia during winter season of 2016. Collected specimens were analyzed for the...

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Autor principal: Alajmi, Reem A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319025/
https://www.ncbi.nlm.nih.gov/pubmed/30622414
http://dx.doi.org/10.1016/j.sjbs.2017.06.010
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author Alajmi, Reem A.
author_facet Alajmi, Reem A.
author_sort Alajmi, Reem A.
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description Fasciolosis is a parasitic disease of medical and economic importance. This retrospective study was conducted on 110 Fasciola flukes collected from livers of 14 infected Naimi sheep slaughtered at Riyadh abattoir in Saudi Arabia during winter season of 2016. Collected specimens were analyzed for their species identification on the basis of partial sequences of mitochondrial 28S rRNA gene. Results have shown the presence of both Fasciola hepatica (F. hepatica) and Fasciola gigantica (F. gigantica) species. Where Fasciola hepatica was predominate (80%). Both intra-species and interspecies genetic distance was studied and results showed that the intraspecific variability among individuals of both species i.e., F. hepatica and F. gigantica, ranging between 0 and 1% while the interspecific diversity between F. hepatica and F. gigantica was only 1%. In conclusion, mitochondrial 28S rRNA gene is a proved as a good marker in identifying Fasciola of different species. Where, the F. hepatica and F. gigantica are present in sheep breed in Riyadh region, Saudi Arabia.
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spelling pubmed-63190252019-01-08 Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep Alajmi, Reem A. Saudi J Biol Sci Article Fasciolosis is a parasitic disease of medical and economic importance. This retrospective study was conducted on 110 Fasciola flukes collected from livers of 14 infected Naimi sheep slaughtered at Riyadh abattoir in Saudi Arabia during winter season of 2016. Collected specimens were analyzed for their species identification on the basis of partial sequences of mitochondrial 28S rRNA gene. Results have shown the presence of both Fasciola hepatica (F. hepatica) and Fasciola gigantica (F. gigantica) species. Where Fasciola hepatica was predominate (80%). Both intra-species and interspecies genetic distance was studied and results showed that the intraspecific variability among individuals of both species i.e., F. hepatica and F. gigantica, ranging between 0 and 1% while the interspecific diversity between F. hepatica and F. gigantica was only 1%. In conclusion, mitochondrial 28S rRNA gene is a proved as a good marker in identifying Fasciola of different species. Where, the F. hepatica and F. gigantica are present in sheep breed in Riyadh region, Saudi Arabia. Elsevier 2019-01 2017-06-20 /pmc/articles/PMC6319025/ /pubmed/30622414 http://dx.doi.org/10.1016/j.sjbs.2017.06.010 Text en © 2017 The Author http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Alajmi, Reem A.
Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep
title Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep
title_full Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep
title_fullStr Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep
title_full_unstemmed Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep
title_short Molecular characterization of Fasciola flukes using mitochondrial 28S rRNA gene in Naimi Saudi sheep
title_sort molecular characterization of fasciola flukes using mitochondrial 28s rrna gene in naimi saudi sheep
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319025/
https://www.ncbi.nlm.nih.gov/pubmed/30622414
http://dx.doi.org/10.1016/j.sjbs.2017.06.010
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