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Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease
BACKGROUND: Niemann-Pick disease type A (NPDA), a disease caused by mutations in acid sphingomyelinase (ASM), involves severe neurodegeneration and early death. Intracellular lipid accumulation and plasma membrane alterations are implicated in the pathology. ASM is also linked to the mechanism of pl...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320626/ https://www.ncbi.nlm.nih.gov/pubmed/30611303 http://dx.doi.org/10.1186/s13395-018-0187-5 |
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author | Michailowsky, V. Li, H. Mittra, B. Iyer, S. R. Mazála, D. A. G. Corrotte, M. Wang, Y. Chin, E. R. Lovering, R. M. Andrews, N. W. |
author_facet | Michailowsky, V. Li, H. Mittra, B. Iyer, S. R. Mazála, D. A. G. Corrotte, M. Wang, Y. Chin, E. R. Lovering, R. M. Andrews, N. W. |
author_sort | Michailowsky, V. |
collection | PubMed |
description | BACKGROUND: Niemann-Pick disease type A (NPDA), a disease caused by mutations in acid sphingomyelinase (ASM), involves severe neurodegeneration and early death. Intracellular lipid accumulation and plasma membrane alterations are implicated in the pathology. ASM is also linked to the mechanism of plasma membrane repair, so we investigated the impact of ASM deficiency in skeletal muscle, a tissue that undergoes frequent cycles of injury and repair in vivo. METHODS: Utilizing the NPDA/B mouse model ASM(−/−) and wild type (WT) littermates, we performed excitation-contraction coupling/Ca(2+) mobilization and sarcolemma injury/repair assays with isolated flexor digitorum brevis fibers, proteomic analyses with quadriceps femoris, flexor digitorum brevis, and tibialis posterior muscle and in vivo tests of the contractile force (maximal isometric torque) of the quadriceps femoris muscle before and after eccentric contraction-induced muscle injury. RESULTS: ASM(−/−) flexor digitorum brevis fibers showed impaired excitation-contraction coupling compared to WT, a defect expressed as reduced tetanic [Ca(2+)](i) in response to electrical stimulation and early failure in sustaining [Ca(2+)](i) during repeated tetanic contractions. When injured mechanically by needle passage, ASM(−/−) flexor digitorum brevis fibers showed susceptibility to injury similar to WT, but a reduced ability to reseal the sarcolemma. Proteomic analyses revealed changes in a small group of skeletal muscle proteins as a consequence of ASM deficiency, with downregulation of calsequestrin occurring in the three different muscles analyzed. In vivo, the loss in maximal isometric torque of WT quadriceps femoris was similar immediately after and 2 min after injury. The loss in ASM(−/−) mice immediately after injury was similar to WT, but was markedly larger at 2 min after injury. CONCLUSIONS: Skeletal muscle fibers from ASM(−/−) mice have an impairment in intracellular Ca(2+) handling that results in reduced Ca(2+) mobilization and a more rapid decline in peak Ca(2+) transients during repeated contraction-relaxation cycles. Isolated fibers show reduced ability to repair damage to the sarcolemma, and this is associated with an exaggerated deficit in force during recovery from an in vivo eccentric contraction-induced muscle injury. Our findings uncover the possibility that skeletal muscle functional defects may play a role in the pathology of NPDA/B disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13395-018-0187-5) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-6320626 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-63206262019-01-09 Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease Michailowsky, V. Li, H. Mittra, B. Iyer, S. R. Mazála, D. A. G. Corrotte, M. Wang, Y. Chin, E. R. Lovering, R. M. Andrews, N. W. Skelet Muscle Research BACKGROUND: Niemann-Pick disease type A (NPDA), a disease caused by mutations in acid sphingomyelinase (ASM), involves severe neurodegeneration and early death. Intracellular lipid accumulation and plasma membrane alterations are implicated in the pathology. ASM is also linked to the mechanism of plasma membrane repair, so we investigated the impact of ASM deficiency in skeletal muscle, a tissue that undergoes frequent cycles of injury and repair in vivo. METHODS: Utilizing the NPDA/B mouse model ASM(−/−) and wild type (WT) littermates, we performed excitation-contraction coupling/Ca(2+) mobilization and sarcolemma injury/repair assays with isolated flexor digitorum brevis fibers, proteomic analyses with quadriceps femoris, flexor digitorum brevis, and tibialis posterior muscle and in vivo tests of the contractile force (maximal isometric torque) of the quadriceps femoris muscle before and after eccentric contraction-induced muscle injury. RESULTS: ASM(−/−) flexor digitorum brevis fibers showed impaired excitation-contraction coupling compared to WT, a defect expressed as reduced tetanic [Ca(2+)](i) in response to electrical stimulation and early failure in sustaining [Ca(2+)](i) during repeated tetanic contractions. When injured mechanically by needle passage, ASM(−/−) flexor digitorum brevis fibers showed susceptibility to injury similar to WT, but a reduced ability to reseal the sarcolemma. Proteomic analyses revealed changes in a small group of skeletal muscle proteins as a consequence of ASM deficiency, with downregulation of calsequestrin occurring in the three different muscles analyzed. In vivo, the loss in maximal isometric torque of WT quadriceps femoris was similar immediately after and 2 min after injury. The loss in ASM(−/−) mice immediately after injury was similar to WT, but was markedly larger at 2 min after injury. CONCLUSIONS: Skeletal muscle fibers from ASM(−/−) mice have an impairment in intracellular Ca(2+) handling that results in reduced Ca(2+) mobilization and a more rapid decline in peak Ca(2+) transients during repeated contraction-relaxation cycles. Isolated fibers show reduced ability to repair damage to the sarcolemma, and this is associated with an exaggerated deficit in force during recovery from an in vivo eccentric contraction-induced muscle injury. Our findings uncover the possibility that skeletal muscle functional defects may play a role in the pathology of NPDA/B disease. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13395-018-0187-5) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-05 /pmc/articles/PMC6320626/ /pubmed/30611303 http://dx.doi.org/10.1186/s13395-018-0187-5 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Michailowsky, V. Li, H. Mittra, B. Iyer, S. R. Mazála, D. A. G. Corrotte, M. Wang, Y. Chin, E. R. Lovering, R. M. Andrews, N. W. Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease |
title | Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease |
title_full | Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease |
title_fullStr | Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease |
title_full_unstemmed | Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease |
title_short | Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease |
title_sort | defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of niemann-pick type a/b disease |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320626/ https://www.ncbi.nlm.nih.gov/pubmed/30611303 http://dx.doi.org/10.1186/s13395-018-0187-5 |
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