Cargando…

MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1

BACKGROUND: MicroRNAs (miRNAs) have been widely recognized as essential regulators in human cancers, including colorectal cancer (CRC). Whether miR-769 is implicated in CRC progression remains elusive. The present study aimed to determine the function of miR-769 in CRC. MATERIAL/METHODS: MiR-769 exp...

Descripción completa

Detalles Bibliográficos
Autores principales: Han, Chao, Song, Yingming, Lian, Changhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320662/
https://www.ncbi.nlm.nih.gov/pubmed/30565566
http://dx.doi.org/10.12659/MSM.911663
_version_ 1783385269770125312
author Han, Chao
Song, Yingming
Lian, Changhong
author_facet Han, Chao
Song, Yingming
Lian, Changhong
author_sort Han, Chao
collection PubMed
description BACKGROUND: MicroRNAs (miRNAs) have been widely recognized as essential regulators in human cancers, including colorectal cancer (CRC). Whether miR-769 is implicated in CRC progression remains elusive. The present study aimed to determine the function of miR-769 in CRC. MATERIAL/METHODS: MiR-769 expression in CRC tissues and adjacent normal tissues were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization. Kaplan-Meier curve analysis was utilized to determine the association between miR-769 expression and prognosis in CRC patients. The effects of miR-769 overexpression on CRC cell proliferation, cell cycle and invasion were analyzed using Cell Counting Kit-8 (CCK8), fluorescence activated cell sorting (FACS), and Transwell assays. Western blot was utilized to assess the effect of miR-769 on HEY1 expression. RESULTS: MiR-769 expression was decreased in CRC tissues. MiR-769 level was negatively correlated with the prognosis of CRC patients. Additionally, miR-769 overexpression remarkably inhibited cell proliferation, arrested CRC cells in G0 stage, and reduced cellular invasion. As to the mechanism, HEY1 was a direct target of miR-769; HEY1 level was inversely correlated with that of miR-769 in CRC tissues. Finally, overexpression of HEY1 reversed the effects of miR-769 on cell proliferation and invasion in CRC. CONCLUSIONS: Our findings demonstrated that miR-769 suppressed the proliferation and invasion of CRC cells through targeting HEY1, which implied that miR-769 might be a novel therapeutic target for CRC treatment.
format Online
Article
Text
id pubmed-6320662
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher International Scientific Literature, Inc.
record_format MEDLINE/PubMed
spelling pubmed-63206622019-01-25 MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1 Han, Chao Song, Yingming Lian, Changhong Med Sci Monit Lab/In Vitro Research BACKGROUND: MicroRNAs (miRNAs) have been widely recognized as essential regulators in human cancers, including colorectal cancer (CRC). Whether miR-769 is implicated in CRC progression remains elusive. The present study aimed to determine the function of miR-769 in CRC. MATERIAL/METHODS: MiR-769 expression in CRC tissues and adjacent normal tissues were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization. Kaplan-Meier curve analysis was utilized to determine the association between miR-769 expression and prognosis in CRC patients. The effects of miR-769 overexpression on CRC cell proliferation, cell cycle and invasion were analyzed using Cell Counting Kit-8 (CCK8), fluorescence activated cell sorting (FACS), and Transwell assays. Western blot was utilized to assess the effect of miR-769 on HEY1 expression. RESULTS: MiR-769 expression was decreased in CRC tissues. MiR-769 level was negatively correlated with the prognosis of CRC patients. Additionally, miR-769 overexpression remarkably inhibited cell proliferation, arrested CRC cells in G0 stage, and reduced cellular invasion. As to the mechanism, HEY1 was a direct target of miR-769; HEY1 level was inversely correlated with that of miR-769 in CRC tissues. Finally, overexpression of HEY1 reversed the effects of miR-769 on cell proliferation and invasion in CRC. CONCLUSIONS: Our findings demonstrated that miR-769 suppressed the proliferation and invasion of CRC cells through targeting HEY1, which implied that miR-769 might be a novel therapeutic target for CRC treatment. International Scientific Literature, Inc. 2018-12-19 /pmc/articles/PMC6320662/ /pubmed/30565566 http://dx.doi.org/10.12659/MSM.911663 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Han, Chao
Song, Yingming
Lian, Changhong
MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1
title MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1
title_full MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1
title_fullStr MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1
title_full_unstemmed MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1
title_short MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1
title_sort mir-769 inhibits colorectal cancer cell proliferation and invasion by targeting hey1
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320662/
https://www.ncbi.nlm.nih.gov/pubmed/30565566
http://dx.doi.org/10.12659/MSM.911663
work_keys_str_mv AT hanchao mir769inhibitscolorectalcancercellproliferationandinvasionbytargetinghey1
AT songyingming mir769inhibitscolorectalcancercellproliferationandinvasionbytargetinghey1
AT lianchanghong mir769inhibitscolorectalcancercellproliferationandinvasionbytargetinghey1