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MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1
BACKGROUND: MicroRNAs (miRNAs) have been widely recognized as essential regulators in human cancers, including colorectal cancer (CRC). Whether miR-769 is implicated in CRC progression remains elusive. The present study aimed to determine the function of miR-769 in CRC. MATERIAL/METHODS: MiR-769 exp...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320662/ https://www.ncbi.nlm.nih.gov/pubmed/30565566 http://dx.doi.org/10.12659/MSM.911663 |
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author | Han, Chao Song, Yingming Lian, Changhong |
author_facet | Han, Chao Song, Yingming Lian, Changhong |
author_sort | Han, Chao |
collection | PubMed |
description | BACKGROUND: MicroRNAs (miRNAs) have been widely recognized as essential regulators in human cancers, including colorectal cancer (CRC). Whether miR-769 is implicated in CRC progression remains elusive. The present study aimed to determine the function of miR-769 in CRC. MATERIAL/METHODS: MiR-769 expression in CRC tissues and adjacent normal tissues were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization. Kaplan-Meier curve analysis was utilized to determine the association between miR-769 expression and prognosis in CRC patients. The effects of miR-769 overexpression on CRC cell proliferation, cell cycle and invasion were analyzed using Cell Counting Kit-8 (CCK8), fluorescence activated cell sorting (FACS), and Transwell assays. Western blot was utilized to assess the effect of miR-769 on HEY1 expression. RESULTS: MiR-769 expression was decreased in CRC tissues. MiR-769 level was negatively correlated with the prognosis of CRC patients. Additionally, miR-769 overexpression remarkably inhibited cell proliferation, arrested CRC cells in G0 stage, and reduced cellular invasion. As to the mechanism, HEY1 was a direct target of miR-769; HEY1 level was inversely correlated with that of miR-769 in CRC tissues. Finally, overexpression of HEY1 reversed the effects of miR-769 on cell proliferation and invasion in CRC. CONCLUSIONS: Our findings demonstrated that miR-769 suppressed the proliferation and invasion of CRC cells through targeting HEY1, which implied that miR-769 might be a novel therapeutic target for CRC treatment. |
format | Online Article Text |
id | pubmed-6320662 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-63206622019-01-25 MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1 Han, Chao Song, Yingming Lian, Changhong Med Sci Monit Lab/In Vitro Research BACKGROUND: MicroRNAs (miRNAs) have been widely recognized as essential regulators in human cancers, including colorectal cancer (CRC). Whether miR-769 is implicated in CRC progression remains elusive. The present study aimed to determine the function of miR-769 in CRC. MATERIAL/METHODS: MiR-769 expression in CRC tissues and adjacent normal tissues were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and in situ hybridization. Kaplan-Meier curve analysis was utilized to determine the association between miR-769 expression and prognosis in CRC patients. The effects of miR-769 overexpression on CRC cell proliferation, cell cycle and invasion were analyzed using Cell Counting Kit-8 (CCK8), fluorescence activated cell sorting (FACS), and Transwell assays. Western blot was utilized to assess the effect of miR-769 on HEY1 expression. RESULTS: MiR-769 expression was decreased in CRC tissues. MiR-769 level was negatively correlated with the prognosis of CRC patients. Additionally, miR-769 overexpression remarkably inhibited cell proliferation, arrested CRC cells in G0 stage, and reduced cellular invasion. As to the mechanism, HEY1 was a direct target of miR-769; HEY1 level was inversely correlated with that of miR-769 in CRC tissues. Finally, overexpression of HEY1 reversed the effects of miR-769 on cell proliferation and invasion in CRC. CONCLUSIONS: Our findings demonstrated that miR-769 suppressed the proliferation and invasion of CRC cells through targeting HEY1, which implied that miR-769 might be a novel therapeutic target for CRC treatment. International Scientific Literature, Inc. 2018-12-19 /pmc/articles/PMC6320662/ /pubmed/30565566 http://dx.doi.org/10.12659/MSM.911663 Text en © Med Sci Monit, 2018 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Lab/In Vitro Research Han, Chao Song, Yingming Lian, Changhong MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1 |
title | MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1 |
title_full | MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1 |
title_fullStr | MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1 |
title_full_unstemmed | MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1 |
title_short | MiR-769 Inhibits Colorectal Cancer Cell Proliferation and Invasion by Targeting HEY1 |
title_sort | mir-769 inhibits colorectal cancer cell proliferation and invasion by targeting hey1 |
topic | Lab/In Vitro Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320662/ https://www.ncbi.nlm.nih.gov/pubmed/30565566 http://dx.doi.org/10.12659/MSM.911663 |
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