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Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine

Background: Antimicrobial peptides (AMP) play a pivotal role in innate host defense and in immune response. The delineation of new MS-based profiling tools, which are able to produce panels of AMP of the nasal fluid (NF), may be attractive for the discovery of new potential diagnostic markers of res...

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Autores principales: Preianò, Mariaimmacolata, Maggisano, Giuseppina, Murfuni, Maria Stella, Villella, Chiara, Colica, Carmela, Fregola, Annalisa, Pelaia, Corrado, Lombardo, Nicola, Pelaia, Girolamo, Savino, Rocco, Terracciano, Rosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320778/
https://www.ncbi.nlm.nih.gov/pubmed/30545076
http://dx.doi.org/10.3390/ijms19124005
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author Preianò, Mariaimmacolata
Maggisano, Giuseppina
Murfuni, Maria Stella
Villella, Chiara
Colica, Carmela
Fregola, Annalisa
Pelaia, Corrado
Lombardo, Nicola
Pelaia, Girolamo
Savino, Rocco
Terracciano, Rosa
author_facet Preianò, Mariaimmacolata
Maggisano, Giuseppina
Murfuni, Maria Stella
Villella, Chiara
Colica, Carmela
Fregola, Annalisa
Pelaia, Corrado
Lombardo, Nicola
Pelaia, Girolamo
Savino, Rocco
Terracciano, Rosa
author_sort Preianò, Mariaimmacolata
collection PubMed
description Background: Antimicrobial peptides (AMP) play a pivotal role in innate host defense and in immune response. The delineation of new MS-based profiling tools, which are able to produce panels of AMP of the nasal fluid (NF), may be attractive for the discovery of new potential diagnostic markers of respiratory disorders. Methods: Swabs collected NF from healthy patients and from patients with respiratory disorders. We used a fast procedure based on mesoporous silica particles (MPS) to enrich NF in its AMP component in combination with MALDI-TOF/TOF MS as a key tool for rapidly analyzing clinical samples. Results: Reproducible MS peptide fingerprints were generated for each subject and several AMP were detected including (Human Neutrophil Peptides) HNPs, Statherin, Thymosin-β4, Peptide P-D, II-2, β-MSP, SLPI, Lysozyme-C, and their proteo-forms. In particular, Statherin, Thymosin-β4, and Peptide P-D were accurately identified by direct MS/MS sequencing. Examples of applicability of this tool are shown. AMP fingerprints were obtained before and after a nasal polypectomy as well as before and post-treatment with azelastine/fluticasone in one case of allergic rhinitis. Conclusion: The potential of our platform to be implemented by new mesoporous materials for capturing a wider picture of AMP might offer an amazing opportunity for diagnostic clinical studies on individual and population scales.
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spelling pubmed-63207782019-01-07 Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine Preianò, Mariaimmacolata Maggisano, Giuseppina Murfuni, Maria Stella Villella, Chiara Colica, Carmela Fregola, Annalisa Pelaia, Corrado Lombardo, Nicola Pelaia, Girolamo Savino, Rocco Terracciano, Rosa Int J Mol Sci Article Background: Antimicrobial peptides (AMP) play a pivotal role in innate host defense and in immune response. The delineation of new MS-based profiling tools, which are able to produce panels of AMP of the nasal fluid (NF), may be attractive for the discovery of new potential diagnostic markers of respiratory disorders. Methods: Swabs collected NF from healthy patients and from patients with respiratory disorders. We used a fast procedure based on mesoporous silica particles (MPS) to enrich NF in its AMP component in combination with MALDI-TOF/TOF MS as a key tool for rapidly analyzing clinical samples. Results: Reproducible MS peptide fingerprints were generated for each subject and several AMP were detected including (Human Neutrophil Peptides) HNPs, Statherin, Thymosin-β4, Peptide P-D, II-2, β-MSP, SLPI, Lysozyme-C, and their proteo-forms. In particular, Statherin, Thymosin-β4, and Peptide P-D were accurately identified by direct MS/MS sequencing. Examples of applicability of this tool are shown. AMP fingerprints were obtained before and after a nasal polypectomy as well as before and post-treatment with azelastine/fluticasone in one case of allergic rhinitis. Conclusion: The potential of our platform to be implemented by new mesoporous materials for capturing a wider picture of AMP might offer an amazing opportunity for diagnostic clinical studies on individual and population scales. MDPI 2018-12-12 /pmc/articles/PMC6320778/ /pubmed/30545076 http://dx.doi.org/10.3390/ijms19124005 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Preianò, Mariaimmacolata
Maggisano, Giuseppina
Murfuni, Maria Stella
Villella, Chiara
Colica, Carmela
Fregola, Annalisa
Pelaia, Corrado
Lombardo, Nicola
Pelaia, Girolamo
Savino, Rocco
Terracciano, Rosa
Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine
title Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine
title_full Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine
title_fullStr Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine
title_full_unstemmed Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine
title_short Rapid Detection and Identification of Antimicrobial Peptide Fingerprints of Nasal Fluid by Mesoporous Silica Particles and MALDI-TOF/TOF Mass Spectrometry: From the Analytical Approach to the Diagnostic Applicability in Precision Medicine
title_sort rapid detection and identification of antimicrobial peptide fingerprints of nasal fluid by mesoporous silica particles and maldi-tof/tof mass spectrometry: from the analytical approach to the diagnostic applicability in precision medicine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320778/
https://www.ncbi.nlm.nih.gov/pubmed/30545076
http://dx.doi.org/10.3390/ijms19124005
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