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Further Stabilization of Alcalase Immobilized on Glyoxyl Supports: Amination Plus Modification with Glutaraldehyde
Alcalase was immobilized on glyoxyl 4% CL agarose beads. This permitted to have Alcalase preparations with 50% activity retention versus Boc-l-alanine 4-nitrophenyl ester. However, the recovered activity versus casein was under 20% at 50 °C, as it may be expected from the most likely area of the pro...
Autores principales: | Hussain, Fouzia, Arana-Peña, Sara, Morellon-Sterling, Roberto, Barbosa, Oveimar, Ait Braham, Sabrina, Kamal, Shagufta, Fernandez-Lafuente, Roberto |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320783/ https://www.ncbi.nlm.nih.gov/pubmed/30513981 http://dx.doi.org/10.3390/molecules23123188 |
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