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Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings

The aim of this study is to design, develop and evaluate new biohybrid sponges based on polymers (collagen and polyvinyl alcohol) with and without indomethacin as anti-inflammatory drug model to be used for tissue regeneration in wound healing. Type I fibrillar collagen in the form of a gel and diff...

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Autores principales: Marin, Ștefania, Albu Kaya, Mădălina Georgiana, Ghica, Mihaela Violeta, Dinu-Pîrvu, Cristina, Popa, Lăcrămioara, Udeanu, Denisa Ioana, Mihai, Geanina, Enachescu, Marius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320800/
https://www.ncbi.nlm.nih.gov/pubmed/30423969
http://dx.doi.org/10.3390/pharmaceutics10040224
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author Marin, Ștefania
Albu Kaya, Mădălina Georgiana
Ghica, Mihaela Violeta
Dinu-Pîrvu, Cristina
Popa, Lăcrămioara
Udeanu, Denisa Ioana
Mihai, Geanina
Enachescu, Marius
author_facet Marin, Ștefania
Albu Kaya, Mădălina Georgiana
Ghica, Mihaela Violeta
Dinu-Pîrvu, Cristina
Popa, Lăcrămioara
Udeanu, Denisa Ioana
Mihai, Geanina
Enachescu, Marius
author_sort Marin, Ștefania
collection PubMed
description The aim of this study is to design, develop and evaluate new biohybrid sponges based on polymers (collagen and polyvinyl alcohol) with and without indomethacin as anti-inflammatory drug model to be used for tissue regeneration in wound healing. Type I fibrillar collagen in the form of a gel and different concentrations of polyvinyl alcohol were mixed together to prepare composite gels. Both control samples, without indomethacin and with indomethacin, were obtained. All samples were crosslinked with glutaraldehyde. By freeze-drying of hydrogels, the spongious forms (matrices) were obtained. The matrices were characterized by FT-IR spectroscopy, scanning electron microscopy (SEM), water absorption, enzymatic degradation and in vitro indomethacin release. The pharmacological effect of the spongious biohybrid matrices was determined on an experimental model of burns induced to Wistar rats. The SEM images showed a porous structure with interconnected pores. Collagen sponges present a structure with pore sizes between 20 and 200 µm, which became more and more compact with polyvinyl alcohol addition. The FT-IR showed interactions between collagen and polyvinyl alcohol. The enzymatic degradation indicated that the most stable matrix is the one with the ratio 75:25 of collagen:polyvinyl alcohol (ACI75), the other ones being degradable in time. The kinetic data of indomethacin release from matrices were fitted with different kinetic models and highlighted a biphasic release of the drug. Such kinetic profiles are targeted in skin wound healing for which important aspects are impaired inflammation and local pain. The treatment with sponges associated with anti-inflammatory drug had beneficial effects on the healing process in experimentally induced burns compared to the corresponding matrices without indomethacin and the classical treated control group.
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spelling pubmed-63208002019-01-11 Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings Marin, Ștefania Albu Kaya, Mădălina Georgiana Ghica, Mihaela Violeta Dinu-Pîrvu, Cristina Popa, Lăcrămioara Udeanu, Denisa Ioana Mihai, Geanina Enachescu, Marius Pharmaceutics Article The aim of this study is to design, develop and evaluate new biohybrid sponges based on polymers (collagen and polyvinyl alcohol) with and without indomethacin as anti-inflammatory drug model to be used for tissue regeneration in wound healing. Type I fibrillar collagen in the form of a gel and different concentrations of polyvinyl alcohol were mixed together to prepare composite gels. Both control samples, without indomethacin and with indomethacin, were obtained. All samples were crosslinked with glutaraldehyde. By freeze-drying of hydrogels, the spongious forms (matrices) were obtained. The matrices were characterized by FT-IR spectroscopy, scanning electron microscopy (SEM), water absorption, enzymatic degradation and in vitro indomethacin release. The pharmacological effect of the spongious biohybrid matrices was determined on an experimental model of burns induced to Wistar rats. The SEM images showed a porous structure with interconnected pores. Collagen sponges present a structure with pore sizes between 20 and 200 µm, which became more and more compact with polyvinyl alcohol addition. The FT-IR showed interactions between collagen and polyvinyl alcohol. The enzymatic degradation indicated that the most stable matrix is the one with the ratio 75:25 of collagen:polyvinyl alcohol (ACI75), the other ones being degradable in time. The kinetic data of indomethacin release from matrices were fitted with different kinetic models and highlighted a biphasic release of the drug. Such kinetic profiles are targeted in skin wound healing for which important aspects are impaired inflammation and local pain. The treatment with sponges associated with anti-inflammatory drug had beneficial effects on the healing process in experimentally induced burns compared to the corresponding matrices without indomethacin and the classical treated control group. MDPI 2018-11-09 /pmc/articles/PMC6320800/ /pubmed/30423969 http://dx.doi.org/10.3390/pharmaceutics10040224 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marin, Ștefania
Albu Kaya, Mădălina Georgiana
Ghica, Mihaela Violeta
Dinu-Pîrvu, Cristina
Popa, Lăcrămioara
Udeanu, Denisa Ioana
Mihai, Geanina
Enachescu, Marius
Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings
title Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings
title_full Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings
title_fullStr Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings
title_full_unstemmed Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings
title_short Collagen-Polyvinyl Alcohol-Indomethacin Biohybrid Matrices as Wound Dressings
title_sort collagen-polyvinyl alcohol-indomethacin biohybrid matrices as wound dressings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320800/
https://www.ncbi.nlm.nih.gov/pubmed/30423969
http://dx.doi.org/10.3390/pharmaceutics10040224
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