Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species

Bacterial periplasmic binding proteins (PBPs) undergo a pronounced ligand-induced conformational change which can be employed to monitor ligand concentrations. The most common strategy to take advantage of this conformational change for a biosensor design is to use a Förster resonance energy transfe...

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Detalles Bibliográficos
Autores principales: Höfig, Henning, Cerminara, Michele, Ritter, Ilona, Schöne, Antonie, Pohl, Martina, Steffen, Victoria, Walter, Julia, Vergara Dal Pont, Ignacio, Katranidis, Alexandros, Fitter, Jörg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320824/
https://www.ncbi.nlm.nih.gov/pubmed/30486450
http://dx.doi.org/10.3390/molecules23123105

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