Cargando…

Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model

Cost-effective and functionalized scaffolds are in high demand for stem-cell-based regenerative medicine to treat refractory bone defects in craniofacial abnormalities and injuries. One potential strategy is to utilize pharmacological and cost-effective plant polyphenols and biocompatible proteins,...

Descripción completa

Detalles Bibliográficos
Autores principales: Sasayama, Satoshi, Hara, Tomoya, Tanaka, Tomonari, Honda, Yoshitomo, Baba, Shunsuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320852/
https://www.ncbi.nlm.nih.gov/pubmed/30501071
http://dx.doi.org/10.3390/ijms19123803
_version_ 1783385302224601088
author Sasayama, Satoshi
Hara, Tomoya
Tanaka, Tomonari
Honda, Yoshitomo
Baba, Shunsuke
author_facet Sasayama, Satoshi
Hara, Tomoya
Tanaka, Tomonari
Honda, Yoshitomo
Baba, Shunsuke
author_sort Sasayama, Satoshi
collection PubMed
description Cost-effective and functionalized scaffolds are in high demand for stem-cell-based regenerative medicine to treat refractory bone defects in craniofacial abnormalities and injuries. One potential strategy is to utilize pharmacological and cost-effective plant polyphenols and biocompatible proteins, such as gelatin. Nevertheless, the use of chemically modified proteins with plant polyphenols in this strategy has not been standardized. Here, we demonstrated that gelatin chemically modified with epigallocatechin gallate (EGCG), the major catechin isolated from green tea, can be a useful material to induce bone regeneration in a rat congenial cleft-jaw model in vivo when used with/without adipose-derived stem cells or dedifferentiated fat cells. Vacuum-heated gelatin sponges modified with EGCG (vhEGCG-GS) induced superior osteogenesis from these two cell types compared with vacuum-heated gelatin sponges (vhGS). The EGCG-modification converted the water wettability of vhGS to a hydrophilic property (contact angle: 110° to 3.8°) and the zeta potential to a negative surface charge; the modification enhanced the cell adhesion property and promoted calcium phosphate precipitation. These results suggest that the EGCG-modification with chemical synthesis can be a useful platform to modify the physicochemical property of gelatin. This alteration is likely to provide a preferable microenvironment for multipotent progenitor cells, inducing superior bone formation in vivo.
format Online
Article
Text
id pubmed-6320852
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63208522019-01-07 Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model Sasayama, Satoshi Hara, Tomoya Tanaka, Tomonari Honda, Yoshitomo Baba, Shunsuke Int J Mol Sci Article Cost-effective and functionalized scaffolds are in high demand for stem-cell-based regenerative medicine to treat refractory bone defects in craniofacial abnormalities and injuries. One potential strategy is to utilize pharmacological and cost-effective plant polyphenols and biocompatible proteins, such as gelatin. Nevertheless, the use of chemically modified proteins with plant polyphenols in this strategy has not been standardized. Here, we demonstrated that gelatin chemically modified with epigallocatechin gallate (EGCG), the major catechin isolated from green tea, can be a useful material to induce bone regeneration in a rat congenial cleft-jaw model in vivo when used with/without adipose-derived stem cells or dedifferentiated fat cells. Vacuum-heated gelatin sponges modified with EGCG (vhEGCG-GS) induced superior osteogenesis from these two cell types compared with vacuum-heated gelatin sponges (vhGS). The EGCG-modification converted the water wettability of vhGS to a hydrophilic property (contact angle: 110° to 3.8°) and the zeta potential to a negative surface charge; the modification enhanced the cell adhesion property and promoted calcium phosphate precipitation. These results suggest that the EGCG-modification with chemical synthesis can be a useful platform to modify the physicochemical property of gelatin. This alteration is likely to provide a preferable microenvironment for multipotent progenitor cells, inducing superior bone formation in vivo. MDPI 2018-11-29 /pmc/articles/PMC6320852/ /pubmed/30501071 http://dx.doi.org/10.3390/ijms19123803 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sasayama, Satoshi
Hara, Tomoya
Tanaka, Tomonari
Honda, Yoshitomo
Baba, Shunsuke
Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
title Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
title_full Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
title_fullStr Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
title_full_unstemmed Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
title_short Osteogenesis of Multipotent Progenitor Cells using the Epigallocatechin Gallate-Modified Gelatin Sponge Scaffold in the Rat Congenital Cleft-Jaw Model
title_sort osteogenesis of multipotent progenitor cells using the epigallocatechin gallate-modified gelatin sponge scaffold in the rat congenital cleft-jaw model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320852/
https://www.ncbi.nlm.nih.gov/pubmed/30501071
http://dx.doi.org/10.3390/ijms19123803
work_keys_str_mv AT sasayamasatoshi osteogenesisofmultipotentprogenitorcellsusingtheepigallocatechingallatemodifiedgelatinspongescaffoldintheratcongenitalcleftjawmodel
AT haratomoya osteogenesisofmultipotentprogenitorcellsusingtheepigallocatechingallatemodifiedgelatinspongescaffoldintheratcongenitalcleftjawmodel
AT tanakatomonari osteogenesisofmultipotentprogenitorcellsusingtheepigallocatechingallatemodifiedgelatinspongescaffoldintheratcongenitalcleftjawmodel
AT hondayoshitomo osteogenesisofmultipotentprogenitorcellsusingtheepigallocatechingallatemodifiedgelatinspongescaffoldintheratcongenitalcleftjawmodel
AT babashunsuke osteogenesisofmultipotentprogenitorcellsusingtheepigallocatechingallatemodifiedgelatinspongescaffoldintheratcongenitalcleftjawmodel