Cargando…
Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway
Aurantio-obtusin, an anthraquinone compound, isolated from dried seeds of Cassia obtusifolia L. (syn. Senna obtusifolia; Fabaceae) and Cassia tora L. (syn. Senna tora). Although the biological activities of Semen Cassiae have been reported, the anti-inflammatory mechanism of aurantio-obtusin, its ma...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320883/ https://www.ncbi.nlm.nih.gov/pubmed/30486383 http://dx.doi.org/10.3390/molecules23123093 |
_version_ | 1783385310762106880 |
---|---|
author | Hou, Jingyi Gu, Yu Zhao, Shuai Huo, Mengqi Wang, Shifeng Zhang, Yanling Qiao, Yanjiang Li, Xi |
author_facet | Hou, Jingyi Gu, Yu Zhao, Shuai Huo, Mengqi Wang, Shifeng Zhang, Yanling Qiao, Yanjiang Li, Xi |
author_sort | Hou, Jingyi |
collection | PubMed |
description | Aurantio-obtusin, an anthraquinone compound, isolated from dried seeds of Cassia obtusifolia L. (syn. Senna obtusifolia; Fabaceae) and Cassia tora L. (syn. Senna tora). Although the biological activities of Semen Cassiae have been reported, the anti-inflammatory mechanism of aurantio-obtusin, its main compound, on RAW264.7 cells, remained unknown. We investigated the anti-inflammatory effect of aurantio-obtusin on lipopolysaccharide- (LPS)-induced RAW264.7 cells in vitro and elucidated the possible underlying molecular mechanisms. Nitric oxide production (NO) and prostaglandin E(2) (PGE(2)) were measured by the Griess colorimetric method and enzyme-linked immunosorbent assay (ELISA), respectively. Protein expression levels of cyclooxygenase 2 (COX-2) were monitored by cell-based ELISA. Interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) synthesis were analyzed using ELISA. The mRNA expression of nitric oxide synthase (iNOS), COX-2, and the critical pro-inflammatory cytokines (IL-6 and TNF-α) were detected by quantitative real-time PCR. Aurantio-obtusin significantly decreased the production of NO, PGE(2), and inhibited the protein expression of COX-2, TNF-α and IL-6, which were similar to those gene expression of iNOS, COX-2, TNF-α and IL-6 (p < 0.01). Consistent with the pro-inflammatory gene expression, the Aurantio-obtusin efficiently reduced the LPS-induced activation of nuclear factor-κB in RAW264.7 cells. These results suggested that aurantio-obtusin may function as a therapeutic agent and can be considered in the further development of treatments for a variety of inflammatory diseases. Further studies may provide scientific evidence for the use of aurantio-obstusin as a new therapeutic agent for inflammation-related diseases. |
format | Online Article Text |
id | pubmed-6320883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63208832019-01-14 Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway Hou, Jingyi Gu, Yu Zhao, Shuai Huo, Mengqi Wang, Shifeng Zhang, Yanling Qiao, Yanjiang Li, Xi Molecules Article Aurantio-obtusin, an anthraquinone compound, isolated from dried seeds of Cassia obtusifolia L. (syn. Senna obtusifolia; Fabaceae) and Cassia tora L. (syn. Senna tora). Although the biological activities of Semen Cassiae have been reported, the anti-inflammatory mechanism of aurantio-obtusin, its main compound, on RAW264.7 cells, remained unknown. We investigated the anti-inflammatory effect of aurantio-obtusin on lipopolysaccharide- (LPS)-induced RAW264.7 cells in vitro and elucidated the possible underlying molecular mechanisms. Nitric oxide production (NO) and prostaglandin E(2) (PGE(2)) were measured by the Griess colorimetric method and enzyme-linked immunosorbent assay (ELISA), respectively. Protein expression levels of cyclooxygenase 2 (COX-2) were monitored by cell-based ELISA. Interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) synthesis were analyzed using ELISA. The mRNA expression of nitric oxide synthase (iNOS), COX-2, and the critical pro-inflammatory cytokines (IL-6 and TNF-α) were detected by quantitative real-time PCR. Aurantio-obtusin significantly decreased the production of NO, PGE(2), and inhibited the protein expression of COX-2, TNF-α and IL-6, which were similar to those gene expression of iNOS, COX-2, TNF-α and IL-6 (p < 0.01). Consistent with the pro-inflammatory gene expression, the Aurantio-obtusin efficiently reduced the LPS-induced activation of nuclear factor-κB in RAW264.7 cells. These results suggested that aurantio-obtusin may function as a therapeutic agent and can be considered in the further development of treatments for a variety of inflammatory diseases. Further studies may provide scientific evidence for the use of aurantio-obstusin as a new therapeutic agent for inflammation-related diseases. MDPI 2018-11-27 /pmc/articles/PMC6320883/ /pubmed/30486383 http://dx.doi.org/10.3390/molecules23123093 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hou, Jingyi Gu, Yu Zhao, Shuai Huo, Mengqi Wang, Shifeng Zhang, Yanling Qiao, Yanjiang Li, Xi Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway |
title | Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway |
title_full | Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway |
title_fullStr | Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway |
title_full_unstemmed | Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway |
title_short | Anti-Inflammatory Effects of Aurantio-Obtusin from Seed of Cassia obtusifolia L. through Modulation of the NF-κB Pathway |
title_sort | anti-inflammatory effects of aurantio-obtusin from seed of cassia obtusifolia l. through modulation of the nf-κb pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320883/ https://www.ncbi.nlm.nih.gov/pubmed/30486383 http://dx.doi.org/10.3390/molecules23123093 |
work_keys_str_mv | AT houjingyi antiinflammatoryeffectsofaurantioobtusinfromseedofcassiaobtusifolialthroughmodulationofthenfkbpathway AT guyu antiinflammatoryeffectsofaurantioobtusinfromseedofcassiaobtusifolialthroughmodulationofthenfkbpathway AT zhaoshuai antiinflammatoryeffectsofaurantioobtusinfromseedofcassiaobtusifolialthroughmodulationofthenfkbpathway AT huomengqi antiinflammatoryeffectsofaurantioobtusinfromseedofcassiaobtusifolialthroughmodulationofthenfkbpathway AT wangshifeng antiinflammatoryeffectsofaurantioobtusinfromseedofcassiaobtusifolialthroughmodulationofthenfkbpathway AT zhangyanling antiinflammatoryeffectsofaurantioobtusinfromseedofcassiaobtusifolialthroughmodulationofthenfkbpathway AT qiaoyanjiang antiinflammatoryeffectsofaurantioobtusinfromseedofcassiaobtusifolialthroughmodulationofthenfkbpathway AT lixi antiinflammatoryeffectsofaurantioobtusinfromseedofcassiaobtusifolialthroughmodulationofthenfkbpathway |