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Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy

Sinisan has been widely used to treat depression. However, its pharmacologically-effective constituents are largely unknown, and the pharmacological effects and clinical efficacies of Sinisan-containing processed medicinal herbs may change. To address these important issues, we developed an ultra-hi...

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Autores principales: Zhou, Jia, Cai, Hao, Tu, Sicong, Duan, Yu, Pei, Ke, Xu, Yangyang, Liu, Jing, Niu, Minjie, Zhang, Yating, Shen, Lin, Zhou, Qigang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320893/
https://www.ncbi.nlm.nih.gov/pubmed/30501046
http://dx.doi.org/10.3390/molecules23123128
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author Zhou, Jia
Cai, Hao
Tu, Sicong
Duan, Yu
Pei, Ke
Xu, Yangyang
Liu, Jing
Niu, Minjie
Zhang, Yating
Shen, Lin
Zhou, Qigang
author_facet Zhou, Jia
Cai, Hao
Tu, Sicong
Duan, Yu
Pei, Ke
Xu, Yangyang
Liu, Jing
Niu, Minjie
Zhang, Yating
Shen, Lin
Zhou, Qigang
author_sort Zhou, Jia
collection PubMed
description Sinisan has been widely used to treat depression. However, its pharmacologically-effective constituents are largely unknown, and the pharmacological effects and clinical efficacies of Sinisan-containing processed medicinal herbs may change. To address these important issues, we developed an ultra-high performance liquid chromatography coupled with electrospray ionization tandem quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) method coupled with multiple statistical strategies to analyze the compound profiles of Sinisan, including individual herb, herb-pair, and complicated Chinese medicinal formula. As a result, 122 different constituents from individual herb, herb-pair, and complicated Chinese medicinal formula were identified totally. Through the comparison of three progressive levels, it suggests that processing herbal medicine and/or altering medicinal formula compatibility could change herbal chemical constituents, resulting in different pharmacological effects. This is also the first report that saikosaponin h/i and saikosaponin g have been identified in Sinisan.
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spelling pubmed-63208932019-01-14 Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy Zhou, Jia Cai, Hao Tu, Sicong Duan, Yu Pei, Ke Xu, Yangyang Liu, Jing Niu, Minjie Zhang, Yating Shen, Lin Zhou, Qigang Molecules Article Sinisan has been widely used to treat depression. However, its pharmacologically-effective constituents are largely unknown, and the pharmacological effects and clinical efficacies of Sinisan-containing processed medicinal herbs may change. To address these important issues, we developed an ultra-high performance liquid chromatography coupled with electrospray ionization tandem quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) method coupled with multiple statistical strategies to analyze the compound profiles of Sinisan, including individual herb, herb-pair, and complicated Chinese medicinal formula. As a result, 122 different constituents from individual herb, herb-pair, and complicated Chinese medicinal formula were identified totally. Through the comparison of three progressive levels, it suggests that processing herbal medicine and/or altering medicinal formula compatibility could change herbal chemical constituents, resulting in different pharmacological effects. This is also the first report that saikosaponin h/i and saikosaponin g have been identified in Sinisan. MDPI 2018-11-29 /pmc/articles/PMC6320893/ /pubmed/30501046 http://dx.doi.org/10.3390/molecules23123128 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Jia
Cai, Hao
Tu, Sicong
Duan, Yu
Pei, Ke
Xu, Yangyang
Liu, Jing
Niu, Minjie
Zhang, Yating
Shen, Lin
Zhou, Qigang
Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy
title Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy
title_full Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy
title_fullStr Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy
title_full_unstemmed Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy
title_short Identification and Analysis of Compound Profiles of Sinisan Based on ‘Individual Herb, Herb-Pair, Herbal Formula’ before and after Processing Using UHPLC-Q-TOF/MS Coupled with Multiple Statistical Strategy
title_sort identification and analysis of compound profiles of sinisan based on ‘individual herb, herb-pair, herbal formula’ before and after processing using uhplc-q-tof/ms coupled with multiple statistical strategy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320893/
https://www.ncbi.nlm.nih.gov/pubmed/30501046
http://dx.doi.org/10.3390/molecules23123128
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