Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins

Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the o...

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Autores principales: Bozhanova, Nina G., Baranov, Mikhail S., Baleeva, Nadezhda S., Gavrikov, Alexey S., Mishin, Alexander S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320917/
https://www.ncbi.nlm.nih.gov/pubmed/30486502
http://dx.doi.org/10.3390/ijms19123778
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author Bozhanova, Nina G.
Baranov, Mikhail S.
Baleeva, Nadezhda S.
Gavrikov, Alexey S.
Mishin, Alexander S.
author_facet Bozhanova, Nina G.
Baranov, Mikhail S.
Baleeva, Nadezhda S.
Gavrikov, Alexey S.
Mishin, Alexander S.
author_sort Bozhanova, Nina G.
collection PubMed
description Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs.
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spelling pubmed-63209172019-01-07 Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins Bozhanova, Nina G. Baranov, Mikhail S. Baleeva, Nadezhda S. Gavrikov, Alexey S. Mishin, Alexander S. Int J Mol Sci Article Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs. MDPI 2018-11-28 /pmc/articles/PMC6320917/ /pubmed/30486502 http://dx.doi.org/10.3390/ijms19123778 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bozhanova, Nina G.
Baranov, Mikhail S.
Baleeva, Nadezhda S.
Gavrikov, Alexey S.
Mishin, Alexander S.
Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins
title Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins
title_full Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins
title_fullStr Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins
title_full_unstemmed Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins
title_short Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins
title_sort red-shifted aminated derivatives of gfp chromophore for live-cell protein labeling with lipocalins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320917/
https://www.ncbi.nlm.nih.gov/pubmed/30486502
http://dx.doi.org/10.3390/ijms19123778
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