Cargando…
Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice
Most Quantitative Trait Loci (QTL) and gene isolation approaches, such as positional- or map-based cloning, are time-consuming and low-throughput methods. Understanding and detecting the genetic material that controls a phenotype is a key means to functionally analyzing genes as well as to enhance c...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321147/ https://www.ncbi.nlm.nih.gov/pubmed/30545055 http://dx.doi.org/10.3390/ijms19124000 |
_version_ | 1783385372804251648 |
---|---|
author | Zegeye, Workie Anley Zhang, Yingxin Cao, Liyong Cheng, Shihua |
author_facet | Zegeye, Workie Anley Zhang, Yingxin Cao, Liyong Cheng, Shihua |
author_sort | Zegeye, Workie Anley |
collection | PubMed |
description | Most Quantitative Trait Loci (QTL) and gene isolation approaches, such as positional- or map-based cloning, are time-consuming and low-throughput methods. Understanding and detecting the genetic material that controls a phenotype is a key means to functionally analyzing genes as well as to enhance crop agronomic traits. In this regard, high-throughput technologies have great prospects for changing the paradigms of DNA marker revealing, genotyping, and for discovering crop genetics and genomic study. Bulk segregant analysis, based on whole genome resequencing approaches, permits the rapid isolation of the genes or QTL responsible for the causative mutation of the phenotypes. MutMap, MutMap Gap, MutMap+, modified MutMap, and QTL-seq methods are among those approaches that have been confirmed to be fruitful gene mapping approaches for crop plants, such as rice, irrespective of whether the characters are determined by polygenes. As a result, in the present study we reviewed the progress made by all these methods to identify QTL or genes in rice. |
format | Online Article Text |
id | pubmed-6321147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63211472019-01-07 Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice Zegeye, Workie Anley Zhang, Yingxin Cao, Liyong Cheng, Shihua Int J Mol Sci Review Most Quantitative Trait Loci (QTL) and gene isolation approaches, such as positional- or map-based cloning, are time-consuming and low-throughput methods. Understanding and detecting the genetic material that controls a phenotype is a key means to functionally analyzing genes as well as to enhance crop agronomic traits. In this regard, high-throughput technologies have great prospects for changing the paradigms of DNA marker revealing, genotyping, and for discovering crop genetics and genomic study. Bulk segregant analysis, based on whole genome resequencing approaches, permits the rapid isolation of the genes or QTL responsible for the causative mutation of the phenotypes. MutMap, MutMap Gap, MutMap+, modified MutMap, and QTL-seq methods are among those approaches that have been confirmed to be fruitful gene mapping approaches for crop plants, such as rice, irrespective of whether the characters are determined by polygenes. As a result, in the present study we reviewed the progress made by all these methods to identify QTL or genes in rice. MDPI 2018-12-12 /pmc/articles/PMC6321147/ /pubmed/30545055 http://dx.doi.org/10.3390/ijms19124000 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Zegeye, Workie Anley Zhang, Yingxin Cao, Liyong Cheng, Shihua Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice |
title | Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice |
title_full | Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice |
title_fullStr | Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice |
title_full_unstemmed | Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice |
title_short | Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice |
title_sort | whole genome resequencing from bulked populations as a rapid qtl and gene identification method in rice |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321147/ https://www.ncbi.nlm.nih.gov/pubmed/30545055 http://dx.doi.org/10.3390/ijms19124000 |
work_keys_str_mv | AT zegeyeworkieanley wholegenomeresequencingfrombulkedpopulationsasarapidqtlandgeneidentificationmethodinrice AT zhangyingxin wholegenomeresequencingfrombulkedpopulationsasarapidqtlandgeneidentificationmethodinrice AT caoliyong wholegenomeresequencingfrombulkedpopulationsasarapidqtlandgeneidentificationmethodinrice AT chengshihua wholegenomeresequencingfrombulkedpopulationsasarapidqtlandgeneidentificationmethodinrice |