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Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice

Most Quantitative Trait Loci (QTL) and gene isolation approaches, such as positional- or map-based cloning, are time-consuming and low-throughput methods. Understanding and detecting the genetic material that controls a phenotype is a key means to functionally analyzing genes as well as to enhance c...

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Autores principales: Zegeye, Workie Anley, Zhang, Yingxin, Cao, Liyong, Cheng, Shihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321147/
https://www.ncbi.nlm.nih.gov/pubmed/30545055
http://dx.doi.org/10.3390/ijms19124000
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author Zegeye, Workie Anley
Zhang, Yingxin
Cao, Liyong
Cheng, Shihua
author_facet Zegeye, Workie Anley
Zhang, Yingxin
Cao, Liyong
Cheng, Shihua
author_sort Zegeye, Workie Anley
collection PubMed
description Most Quantitative Trait Loci (QTL) and gene isolation approaches, such as positional- or map-based cloning, are time-consuming and low-throughput methods. Understanding and detecting the genetic material that controls a phenotype is a key means to functionally analyzing genes as well as to enhance crop agronomic traits. In this regard, high-throughput technologies have great prospects for changing the paradigms of DNA marker revealing, genotyping, and for discovering crop genetics and genomic study. Bulk segregant analysis, based on whole genome resequencing approaches, permits the rapid isolation of the genes or QTL responsible for the causative mutation of the phenotypes. MutMap, MutMap Gap, MutMap+, modified MutMap, and QTL-seq methods are among those approaches that have been confirmed to be fruitful gene mapping approaches for crop plants, such as rice, irrespective of whether the characters are determined by polygenes. As a result, in the present study we reviewed the progress made by all these methods to identify QTL or genes in rice.
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spelling pubmed-63211472019-01-07 Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice Zegeye, Workie Anley Zhang, Yingxin Cao, Liyong Cheng, Shihua Int J Mol Sci Review Most Quantitative Trait Loci (QTL) and gene isolation approaches, such as positional- or map-based cloning, are time-consuming and low-throughput methods. Understanding and detecting the genetic material that controls a phenotype is a key means to functionally analyzing genes as well as to enhance crop agronomic traits. In this regard, high-throughput technologies have great prospects for changing the paradigms of DNA marker revealing, genotyping, and for discovering crop genetics and genomic study. Bulk segregant analysis, based on whole genome resequencing approaches, permits the rapid isolation of the genes or QTL responsible for the causative mutation of the phenotypes. MutMap, MutMap Gap, MutMap+, modified MutMap, and QTL-seq methods are among those approaches that have been confirmed to be fruitful gene mapping approaches for crop plants, such as rice, irrespective of whether the characters are determined by polygenes. As a result, in the present study we reviewed the progress made by all these methods to identify QTL or genes in rice. MDPI 2018-12-12 /pmc/articles/PMC6321147/ /pubmed/30545055 http://dx.doi.org/10.3390/ijms19124000 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Zegeye, Workie Anley
Zhang, Yingxin
Cao, Liyong
Cheng, Shihua
Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice
title Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice
title_full Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice
title_fullStr Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice
title_full_unstemmed Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice
title_short Whole Genome Resequencing from Bulked Populations as a Rapid QTL and Gene Identification Method in Rice
title_sort whole genome resequencing from bulked populations as a rapid qtl and gene identification method in rice
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321147/
https://www.ncbi.nlm.nih.gov/pubmed/30545055
http://dx.doi.org/10.3390/ijms19124000
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