Cargando…

Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line

Primary adipose tissue-derived multipotent stem/stromal cells (adMSCs) demonstrate unusual signaling regulatory mechanisms, i.e., increased of sensitivity to catecholamines in response to noradrenaline. This phenomenon is called “heterologous sensitization”, and was previously found only in embryoni...

Descripción completa

Detalles Bibliográficos
Autores principales: Tyurin-Kuzmin, Pyotr A., Chechekhin, Vadim I., Ivanova, Anastasiya M., Dyikanov, Daniyar T., Sysoeva, Veronika Y., Kalinina, Natalia I., Tkachuk, Vsevolod A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321284/
https://www.ncbi.nlm.nih.gov/pubmed/30469522
http://dx.doi.org/10.3390/ijms19123712
_version_ 1783385405357293568
author Tyurin-Kuzmin, Pyotr A.
Chechekhin, Vadim I.
Ivanova, Anastasiya M.
Dyikanov, Daniyar T.
Sysoeva, Veronika Y.
Kalinina, Natalia I.
Tkachuk, Vsevolod A.
author_facet Tyurin-Kuzmin, Pyotr A.
Chechekhin, Vadim I.
Ivanova, Anastasiya M.
Dyikanov, Daniyar T.
Sysoeva, Veronika Y.
Kalinina, Natalia I.
Tkachuk, Vsevolod A.
author_sort Tyurin-Kuzmin, Pyotr A.
collection PubMed
description Primary adipose tissue-derived multipotent stem/stromal cells (adMSCs) demonstrate unusual signaling regulatory mechanisms, i.e., increased of sensitivity to catecholamines in response to noradrenaline. This phenomenon is called “heterologous sensitization”, and was previously found only in embryonic cells. Since further elucidation of the molecular mechanisms that are responsible for such sensitization in primary adMSCs was difficult due to the high heterogeneity in adrenergic receptor expression, we employed immortalized adipose-derived mesenchymal stem cell lines (hTERT-MSCs). Using flow cytometry and immunofluorescence microscopy, we demonstrated that the proportion of cells expressing adrenergic receptor isoforms does not differ significantly in hTERT-MSCs cells compared to the primary adMSCs culture. However, using analysis of Ca(2+)-mobilization in single cells, we found that these cells did not demonstrate the sensitization seen in primary adMSCs. Consistently, these cells did not activate cAMP synthesis in response to noradrenaline. These data indicate that immortalized adipose-derived mesenchymal stem cell lines demonstrated impaired ability to respond to noradrenaline compared to primary adMSCs. These data draw attention to the usage of immortalized cells for MSCs-based regenerative medicine, especially in the field of pharmacology.
format Online
Article
Text
id pubmed-6321284
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63212842019-01-07 Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line Tyurin-Kuzmin, Pyotr A. Chechekhin, Vadim I. Ivanova, Anastasiya M. Dyikanov, Daniyar T. Sysoeva, Veronika Y. Kalinina, Natalia I. Tkachuk, Vsevolod A. Int J Mol Sci Communication Primary adipose tissue-derived multipotent stem/stromal cells (adMSCs) demonstrate unusual signaling regulatory mechanisms, i.e., increased of sensitivity to catecholamines in response to noradrenaline. This phenomenon is called “heterologous sensitization”, and was previously found only in embryonic cells. Since further elucidation of the molecular mechanisms that are responsible for such sensitization in primary adMSCs was difficult due to the high heterogeneity in adrenergic receptor expression, we employed immortalized adipose-derived mesenchymal stem cell lines (hTERT-MSCs). Using flow cytometry and immunofluorescence microscopy, we demonstrated that the proportion of cells expressing adrenergic receptor isoforms does not differ significantly in hTERT-MSCs cells compared to the primary adMSCs culture. However, using analysis of Ca(2+)-mobilization in single cells, we found that these cells did not demonstrate the sensitization seen in primary adMSCs. Consistently, these cells did not activate cAMP synthesis in response to noradrenaline. These data indicate that immortalized adipose-derived mesenchymal stem cell lines demonstrated impaired ability to respond to noradrenaline compared to primary adMSCs. These data draw attention to the usage of immortalized cells for MSCs-based regenerative medicine, especially in the field of pharmacology. MDPI 2018-11-22 /pmc/articles/PMC6321284/ /pubmed/30469522 http://dx.doi.org/10.3390/ijms19123712 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Tyurin-Kuzmin, Pyotr A.
Chechekhin, Vadim I.
Ivanova, Anastasiya M.
Dyikanov, Daniyar T.
Sysoeva, Veronika Y.
Kalinina, Natalia I.
Tkachuk, Vsevolod A.
Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line
title Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line
title_full Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line
title_fullStr Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line
title_full_unstemmed Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line
title_short Noradrenaline Sensitivity Is Severely Impaired in Immortalized Adipose-Derived Mesenchymal Stem Cell Line
title_sort noradrenaline sensitivity is severely impaired in immortalized adipose-derived mesenchymal stem cell line
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321284/
https://www.ncbi.nlm.nih.gov/pubmed/30469522
http://dx.doi.org/10.3390/ijms19123712
work_keys_str_mv AT tyurinkuzminpyotra noradrenalinesensitivityisseverelyimpairedinimmortalizedadiposederivedmesenchymalstemcellline
AT chechekhinvadimi noradrenalinesensitivityisseverelyimpairedinimmortalizedadiposederivedmesenchymalstemcellline
AT ivanovaanastasiyam noradrenalinesensitivityisseverelyimpairedinimmortalizedadiposederivedmesenchymalstemcellline
AT dyikanovdaniyart noradrenalinesensitivityisseverelyimpairedinimmortalizedadiposederivedmesenchymalstemcellline
AT sysoevaveronikay noradrenalinesensitivityisseverelyimpairedinimmortalizedadiposederivedmesenchymalstemcellline
AT kalininanataliai noradrenalinesensitivityisseverelyimpairedinimmortalizedadiposederivedmesenchymalstemcellline
AT tkachukvsevoloda noradrenalinesensitivityisseverelyimpairedinimmortalizedadiposederivedmesenchymalstemcellline