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Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats

Inflammatory bowel disease is triggered by an uncontrolled immune response associated with genetic, environmental, and intestinal microbiota imbalance. Ipomoea asarifolia (IA), popularly known as “salsa” or “brave salsa”, belongs to the Convolvulaceae family. The aim of this approach was to study th...

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Autores principales: da Silva, Valéria Costa, de Araújo, Aurigena Antunes, Araújo, Daline Fernandes de Souza, Lima, Maíra Conceição Jerônimo Souza, Vasconcelos, Roseane Carvalho, de Araújo Júnior, Raimundo Fernandes, Langasnner, Silvana Maria Zucolotto, Pedrosa, Matheus de Freitas Fernandes, de Medeiros, Caroline Addison Carvalho Xavier, Guerra, Gerlane Coelho Bernardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321343/
https://www.ncbi.nlm.nih.gov/pubmed/30545135
http://dx.doi.org/10.3390/ijms19124016
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author da Silva, Valéria Costa
de Araújo, Aurigena Antunes
Araújo, Daline Fernandes de Souza
Lima, Maíra Conceição Jerônimo Souza
Vasconcelos, Roseane Carvalho
de Araújo Júnior, Raimundo Fernandes
Langasnner, Silvana Maria Zucolotto
Pedrosa, Matheus de Freitas Fernandes
de Medeiros, Caroline Addison Carvalho Xavier
Guerra, Gerlane Coelho Bernardo
author_facet da Silva, Valéria Costa
de Araújo, Aurigena Antunes
Araújo, Daline Fernandes de Souza
Lima, Maíra Conceição Jerônimo Souza
Vasconcelos, Roseane Carvalho
de Araújo Júnior, Raimundo Fernandes
Langasnner, Silvana Maria Zucolotto
Pedrosa, Matheus de Freitas Fernandes
de Medeiros, Caroline Addison Carvalho Xavier
Guerra, Gerlane Coelho Bernardo
author_sort da Silva, Valéria Costa
collection PubMed
description Inflammatory bowel disease is triggered by an uncontrolled immune response associated with genetic, environmental, and intestinal microbiota imbalance. Ipomoea asarifolia (IA), popularly known as “salsa” or “brave salsa”, belongs to the Convolvulaceae family. The aim of this approach was to study the preventive effect of IA aqueous extract in 2,4-dinitrobenzene sulfonic acid (DNBS)-induced colitis in rats. Rats pretreated with IA extract or sulfasalazine (SSZ) received intracolonic instillation of DNBS in 50% ethanol (v/v). IA extract presented a protective effect against intestinal inflammation, with improvement in the disease activity index and macroscopic damage. IA or SSZ significantly reduced myeloperoxidase activity, and also down-regulation of the gene expression of JNK1, NF-κβ-p65, STAT3, and decreased levels of TNFα, IL-1β, and increased IL-10, associated with a significant improvement of oxidative stress, in addition to a reduction in MDA and an increase of glutathione in colonic tissue. The protective effect of the extract was also confirmed in histological evaluation, showing preservation of the colonic cytoarchitecture. Immunohistochemical analysis revealed down-regulation of NF-κβ-p65, iNOS, IL-17, and up-regulation of SOCs-1 and MUC-2. IA extract presents antioxidant and anti-inflammatory intestinal properties, and proved to be a potential application for preventing damage induced by DNBS.
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spelling pubmed-63213432019-01-07 Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats da Silva, Valéria Costa de Araújo, Aurigena Antunes Araújo, Daline Fernandes de Souza Lima, Maíra Conceição Jerônimo Souza Vasconcelos, Roseane Carvalho de Araújo Júnior, Raimundo Fernandes Langasnner, Silvana Maria Zucolotto Pedrosa, Matheus de Freitas Fernandes de Medeiros, Caroline Addison Carvalho Xavier Guerra, Gerlane Coelho Bernardo Int J Mol Sci Article Inflammatory bowel disease is triggered by an uncontrolled immune response associated with genetic, environmental, and intestinal microbiota imbalance. Ipomoea asarifolia (IA), popularly known as “salsa” or “brave salsa”, belongs to the Convolvulaceae family. The aim of this approach was to study the preventive effect of IA aqueous extract in 2,4-dinitrobenzene sulfonic acid (DNBS)-induced colitis in rats. Rats pretreated with IA extract or sulfasalazine (SSZ) received intracolonic instillation of DNBS in 50% ethanol (v/v). IA extract presented a protective effect against intestinal inflammation, with improvement in the disease activity index and macroscopic damage. IA or SSZ significantly reduced myeloperoxidase activity, and also down-regulation of the gene expression of JNK1, NF-κβ-p65, STAT3, and decreased levels of TNFα, IL-1β, and increased IL-10, associated with a significant improvement of oxidative stress, in addition to a reduction in MDA and an increase of glutathione in colonic tissue. The protective effect of the extract was also confirmed in histological evaluation, showing preservation of the colonic cytoarchitecture. Immunohistochemical analysis revealed down-regulation of NF-κβ-p65, iNOS, IL-17, and up-regulation of SOCs-1 and MUC-2. IA extract presents antioxidant and anti-inflammatory intestinal properties, and proved to be a potential application for preventing damage induced by DNBS. MDPI 2018-12-12 /pmc/articles/PMC6321343/ /pubmed/30545135 http://dx.doi.org/10.3390/ijms19124016 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva, Valéria Costa
de Araújo, Aurigena Antunes
Araújo, Daline Fernandes de Souza
Lima, Maíra Conceição Jerônimo Souza
Vasconcelos, Roseane Carvalho
de Araújo Júnior, Raimundo Fernandes
Langasnner, Silvana Maria Zucolotto
Pedrosa, Matheus de Freitas Fernandes
de Medeiros, Caroline Addison Carvalho Xavier
Guerra, Gerlane Coelho Bernardo
Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats
title Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats
title_full Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats
title_fullStr Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats
title_full_unstemmed Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats
title_short Intestinal Anti-Inflammatory Activity of the Aqueous Extract from Ipomoea asarifolia in DNBS-Induced Colitis in Rats
title_sort intestinal anti-inflammatory activity of the aqueous extract from ipomoea asarifolia in dnbs-induced colitis in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321343/
https://www.ncbi.nlm.nih.gov/pubmed/30545135
http://dx.doi.org/10.3390/ijms19124016
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