Cargando…
Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction
Three phenylethanoid glycosides, echinacoside (1), salidroside (3), and acteoside (6), and three secoiridoid glycosides, isonuezhenide (2), nuezhenoside G13 (4), and specnuezhenide (5), have been extracted and separated by a combined method of ultrahigh pressure extraction (UPE) and high-speed count...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321428/ https://www.ncbi.nlm.nih.gov/pubmed/30567348 http://dx.doi.org/10.3390/molecules23123353 |
_version_ | 1783385440098713600 |
---|---|
author | He, Fengwei Chen, Li Liu, Qian Wang, Xiao Li, Jia Yu, Jinqian |
author_facet | He, Fengwei Chen, Li Liu, Qian Wang, Xiao Li, Jia Yu, Jinqian |
author_sort | He, Fengwei |
collection | PubMed |
description | Three phenylethanoid glycosides, echinacoside (1), salidroside (3), and acteoside (6), and three secoiridoid glycosides, isonuezhenide (2), nuezhenoside G13 (4), and specnuezhenide (5), have been extracted and separated by a combined method of ultrahigh pressure extraction (UPE) and high-speed counter-current chromatography (HSCCC) from Ligustri Lucidi Fructus. For the UPE, the optimal extraction was developed with conditions including solvent of 90% ethanol, sample to solvent ratio of 1:20 g/mL, pressure of 200 MPa, and time of 2 min, which rendered the yields of compounds 4 and 5 were 15.0 and 78.0 mg/g, respectively. For the HSCCC separation, the strategy of changing flow rates between 1.0 and 2.0 mL/min allowed the acquisition for 2.7 mg of compound 1, 4.5 mg of compound 2, 6.8 mg of compound 3, 5.9 mg of compound 4, 11.2 mg of compound 5, and 2.2 mg of compound 6 in one separation run under the solvent system of ethyl acetate:n-butanol:water (2:1:3, v/v) from 200 mg of the UPE extract. The structures of these phenylethanoid and secoiridoid glycosides were elucidated by extensive spectroscopic methods. |
format | Online Article Text |
id | pubmed-6321428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63214282019-01-14 Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction He, Fengwei Chen, Li Liu, Qian Wang, Xiao Li, Jia Yu, Jinqian Molecules Article Three phenylethanoid glycosides, echinacoside (1), salidroside (3), and acteoside (6), and three secoiridoid glycosides, isonuezhenide (2), nuezhenoside G13 (4), and specnuezhenide (5), have been extracted and separated by a combined method of ultrahigh pressure extraction (UPE) and high-speed counter-current chromatography (HSCCC) from Ligustri Lucidi Fructus. For the UPE, the optimal extraction was developed with conditions including solvent of 90% ethanol, sample to solvent ratio of 1:20 g/mL, pressure of 200 MPa, and time of 2 min, which rendered the yields of compounds 4 and 5 were 15.0 and 78.0 mg/g, respectively. For the HSCCC separation, the strategy of changing flow rates between 1.0 and 2.0 mL/min allowed the acquisition for 2.7 mg of compound 1, 4.5 mg of compound 2, 6.8 mg of compound 3, 5.9 mg of compound 4, 11.2 mg of compound 5, and 2.2 mg of compound 6 in one separation run under the solvent system of ethyl acetate:n-butanol:water (2:1:3, v/v) from 200 mg of the UPE extract. The structures of these phenylethanoid and secoiridoid glycosides were elucidated by extensive spectroscopic methods. MDPI 2018-12-18 /pmc/articles/PMC6321428/ /pubmed/30567348 http://dx.doi.org/10.3390/molecules23123353 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article He, Fengwei Chen, Li Liu, Qian Wang, Xiao Li, Jia Yu, Jinqian Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction |
title | Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction |
title_full | Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction |
title_fullStr | Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction |
title_full_unstemmed | Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction |
title_short | Preparative Separation of Phenylethanoid and Secoiridoid Glycosides from Ligustri Lucidi Fructus by High-Speed Counter-Current Chromatography Coupled with Ultrahigh Pressure Extraction |
title_sort | preparative separation of phenylethanoid and secoiridoid glycosides from ligustri lucidi fructus by high-speed counter-current chromatography coupled with ultrahigh pressure extraction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321428/ https://www.ncbi.nlm.nih.gov/pubmed/30567348 http://dx.doi.org/10.3390/molecules23123353 |
work_keys_str_mv | AT hefengwei preparativeseparationofphenylethanoidandsecoiridoidglycosidesfromligustrilucidifructusbyhighspeedcountercurrentchromatographycoupledwithultrahighpressureextraction AT chenli preparativeseparationofphenylethanoidandsecoiridoidglycosidesfromligustrilucidifructusbyhighspeedcountercurrentchromatographycoupledwithultrahighpressureextraction AT liuqian preparativeseparationofphenylethanoidandsecoiridoidglycosidesfromligustrilucidifructusbyhighspeedcountercurrentchromatographycoupledwithultrahighpressureextraction AT wangxiao preparativeseparationofphenylethanoidandsecoiridoidglycosidesfromligustrilucidifructusbyhighspeedcountercurrentchromatographycoupledwithultrahighpressureextraction AT lijia preparativeseparationofphenylethanoidandsecoiridoidglycosidesfromligustrilucidifructusbyhighspeedcountercurrentchromatographycoupledwithultrahighpressureextraction AT yujinqian preparativeseparationofphenylethanoidandsecoiridoidglycosidesfromligustrilucidifructusbyhighspeedcountercurrentchromatographycoupledwithultrahighpressureextraction |