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Daidzein-Stimulated Increase in the Ciliary Beating Amplitude via an [Cl(−)](i) Decrease in Ciliated Human Nasal Epithelial Cells

The effects of the isoflavone daidzein on the ciliary beat distance (CBD, which is a parameter assessing the amplitude of ciliary beating) and the ciliary beat frequency (CBF) were examined in ciliated human nasal epithelial cells (cHNECs) in primary culture. Daidzein decreased [Cl(−)](i) and enhanc...

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Detalles Bibliográficos
Autores principales: Inui, Taka-aki, Yasuda, Makoto, Hirano, Shigeru, Ikeuchi, Yukiko, Kogiso, Haruka, Inui, Toshio, Marunaka, Yoshinori, Nakahari, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321431/
https://www.ncbi.nlm.nih.gov/pubmed/30486295
http://dx.doi.org/10.3390/ijms19123754
Descripción
Sumario:The effects of the isoflavone daidzein on the ciliary beat distance (CBD, which is a parameter assessing the amplitude of ciliary beating) and the ciliary beat frequency (CBF) were examined in ciliated human nasal epithelial cells (cHNECs) in primary culture. Daidzein decreased [Cl(−)](i) and enhanced CBD in cHNECs. The CBD increase that was stimulated by daidzein was mimicked by Cl(−)-free NO(3)(−) solution and bumetanide (an inhibitor of Na(+)/K(+)/2Cl(−) cotransport), both of which decreased [Cl(−)](i.) Moreover, the CBD increase was inhibited by 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, a Cl(−) channel blocker), which increased [Cl(−)](i). CBF was also decreased by NPPB. The rate of [Cl(−)](i) decrease evoked by Cl(−)-free NO(3)(−) solution was enhanced by daidzein. These results suggest that daidzein activates Cl(−) channels in cHNECs. Moreover, daidzein enhanced the microbead transport driven by beating cilia in the cell sheet of cHNECs, suggesting that an increase in CBD enhances ciliary transport. An [Cl(−)](i) decrease enhanced CBD, but not CBF, in cHNECs at 37 °C, although it enhanced both at 25 °C. Intracellular Cl(−) affects both CBD and CBF in a temperature-dependent manner. In conclusion, daidzein, which activates Cl(−) channels to decrease [Cl(−)](i), stimulated CBD increase in cHNECs at 37 °C. CBD is a crucial factor that can increase ciliary transport in the airways under physiological conditions.