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Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells
Gastrin-releasing peptide (GRP), a member of bombesin-like peptides, and its receptor (GRP-R) play an important role in various physiological and pathological conditions. In this work, we investigated the role of GRP-R on adipogenesis in 3T3-L1 adipocytes. The expression of GRP-R was significantly i...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321486/ https://www.ncbi.nlm.nih.gov/pubmed/30544709 http://dx.doi.org/10.3390/ijms19123971 |
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author | Kim, Mi-Kyoung Park, Hyun-Joo Kim, Yeon Bae, Soo-Kyung Kim, Hyung Joon Bae, Moon-Kyoung |
author_facet | Kim, Mi-Kyoung Park, Hyun-Joo Kim, Yeon Bae, Soo-Kyung Kim, Hyung Joon Bae, Moon-Kyoung |
author_sort | Kim, Mi-Kyoung |
collection | PubMed |
description | Gastrin-releasing peptide (GRP), a member of bombesin-like peptides, and its receptor (GRP-R) play an important role in various physiological and pathological conditions. In this work, we investigated the role of GRP-R on adipogenesis in 3T3-L1 adipocytes. The expression of GRP-R was significantly increased during the adipocyte differentiation of 3T3-L1 cells. The inhibition of GRP-R by the antagonist RC-3095 affected adipogenesis in 3T3-L1 cells, which reduced lipid accumulation and regulated the expression of adipogenic genes. Moreover, cyclic AMP response element-binding protein (CREB) directly bound to the GRP-R promoter upon exposure to adipogenic stimuli. The down-regulation of GRP-R by the knockdown of CREB inhibited adipocyte differentiation of 3T3-L1 cells. Together these results suggest that the regulation of GRP-R activity or expression has an influence on adipogenesis through regulating adipogenic related genes. |
format | Online Article Text |
id | pubmed-6321486 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63214862019-01-07 Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells Kim, Mi-Kyoung Park, Hyun-Joo Kim, Yeon Bae, Soo-Kyung Kim, Hyung Joon Bae, Moon-Kyoung Int J Mol Sci Article Gastrin-releasing peptide (GRP), a member of bombesin-like peptides, and its receptor (GRP-R) play an important role in various physiological and pathological conditions. In this work, we investigated the role of GRP-R on adipogenesis in 3T3-L1 adipocytes. The expression of GRP-R was significantly increased during the adipocyte differentiation of 3T3-L1 cells. The inhibition of GRP-R by the antagonist RC-3095 affected adipogenesis in 3T3-L1 cells, which reduced lipid accumulation and regulated the expression of adipogenic genes. Moreover, cyclic AMP response element-binding protein (CREB) directly bound to the GRP-R promoter upon exposure to adipogenic stimuli. The down-regulation of GRP-R by the knockdown of CREB inhibited adipocyte differentiation of 3T3-L1 cells. Together these results suggest that the regulation of GRP-R activity or expression has an influence on adipogenesis through regulating adipogenic related genes. MDPI 2018-12-10 /pmc/articles/PMC6321486/ /pubmed/30544709 http://dx.doi.org/10.3390/ijms19123971 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kim, Mi-Kyoung Park, Hyun-Joo Kim, Yeon Bae, Soo-Kyung Kim, Hyung Joon Bae, Moon-Kyoung Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells |
title | Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells |
title_full | Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells |
title_fullStr | Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells |
title_full_unstemmed | Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells |
title_short | Involvement of Gastrin-Releasing Peptide Receptor in the Regulation of Adipocyte Differentiation in 3T3-L1 Cells |
title_sort | involvement of gastrin-releasing peptide receptor in the regulation of adipocyte differentiation in 3t3-l1 cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321486/ https://www.ncbi.nlm.nih.gov/pubmed/30544709 http://dx.doi.org/10.3390/ijms19123971 |
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