Cargando…
Interactions of Bromocarbazoles with Human Serum Albumin Using Spectroscopic Methods
The 1,3,6,8-tetrabromocarbazole and 3-bromocarbazole have attracted great attention in the ecotoxicology field recently as hazardous environmental contaminants. In this study, the quenching mechanism of these two substances binding with human serum albumin (HSA) has been investigated with spectrosco...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321538/ https://www.ncbi.nlm.nih.gov/pubmed/30487451 http://dx.doi.org/10.3390/molecules23123120 |
_version_ | 1783385466422165504 |
---|---|
author | Yan, Xiaodan Yuan, Dongjie Pan, Dandan |
author_facet | Yan, Xiaodan Yuan, Dongjie Pan, Dandan |
author_sort | Yan, Xiaodan |
collection | PubMed |
description | The 1,3,6,8-tetrabromocarbazole and 3-bromocarbazole have attracted great attention in the ecotoxicology field recently as hazardous environmental contaminants. In this study, the quenching mechanism of these two substances binding with human serum albumin (HSA) has been investigated with spectroscopic methods. Through fluorescence quenching and binding site experiments with steady-state fluorescence and UV-Vis spectra, the intrinsic fluorescence of HSA quenched by 1,3,6,8-tetrabromocarbazole and 3-bromocarbazole both in static process, are activated by binding to site II (subdomain IIIA) of the HSA. In addition, it was not only found that the conformation and secondary structure of the proteins changes, but also that their spontaneous binding processes were driven by electrostatic interactions as well as hydrophobic forces for HSA-1,3,6,8-tetrabromocarbazole, and by typical hydrophobic forces for HSA-3-bromocarbazole. The above studies are beneficial to enhance our understanding of the ecotoxicology and environmental behaviors of halogenated carbazoles. |
format | Online Article Text |
id | pubmed-6321538 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63215382019-01-14 Interactions of Bromocarbazoles with Human Serum Albumin Using Spectroscopic Methods Yan, Xiaodan Yuan, Dongjie Pan, Dandan Molecules Article The 1,3,6,8-tetrabromocarbazole and 3-bromocarbazole have attracted great attention in the ecotoxicology field recently as hazardous environmental contaminants. In this study, the quenching mechanism of these two substances binding with human serum albumin (HSA) has been investigated with spectroscopic methods. Through fluorescence quenching and binding site experiments with steady-state fluorescence and UV-Vis spectra, the intrinsic fluorescence of HSA quenched by 1,3,6,8-tetrabromocarbazole and 3-bromocarbazole both in static process, are activated by binding to site II (subdomain IIIA) of the HSA. In addition, it was not only found that the conformation and secondary structure of the proteins changes, but also that their spontaneous binding processes were driven by electrostatic interactions as well as hydrophobic forces for HSA-1,3,6,8-tetrabromocarbazole, and by typical hydrophobic forces for HSA-3-bromocarbazole. The above studies are beneficial to enhance our understanding of the ecotoxicology and environmental behaviors of halogenated carbazoles. MDPI 2018-11-28 /pmc/articles/PMC6321538/ /pubmed/30487451 http://dx.doi.org/10.3390/molecules23123120 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yan, Xiaodan Yuan, Dongjie Pan, Dandan Interactions of Bromocarbazoles with Human Serum Albumin Using Spectroscopic Methods |
title | Interactions of Bromocarbazoles with Human Serum Albumin Using Spectroscopic Methods |
title_full | Interactions of Bromocarbazoles with Human Serum Albumin Using Spectroscopic Methods |
title_fullStr | Interactions of Bromocarbazoles with Human Serum Albumin Using Spectroscopic Methods |
title_full_unstemmed | Interactions of Bromocarbazoles with Human Serum Albumin Using Spectroscopic Methods |
title_short | Interactions of Bromocarbazoles with Human Serum Albumin Using Spectroscopic Methods |
title_sort | interactions of bromocarbazoles with human serum albumin using spectroscopic methods |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321538/ https://www.ncbi.nlm.nih.gov/pubmed/30487451 http://dx.doi.org/10.3390/molecules23123120 |
work_keys_str_mv | AT yanxiaodan interactionsofbromocarbazoleswithhumanserumalbuminusingspectroscopicmethods AT yuandongjie interactionsofbromocarbazoleswithhumanserumalbuminusingspectroscopicmethods AT pandandan interactionsofbromocarbazoleswithhumanserumalbuminusingspectroscopicmethods |