Cargando…

Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development

Gastric cancer (GC) is the fifth most common cancer and the third most frequent cause of cancer deaths worldwide. The high death rate associated with GC, and lack of appropriate biomarkers for diagnosis, prognosis, and treatment emphasize the need for identification of novel molecules. Given the eme...

Descripción completa

Detalles Bibliográficos
Autores principales: Foroughi, Kobra, Amini, Mohammad, Atashi, Amir, Mahmoodzadeh, Habibollah, Hamann, Ute, Manoochehri, Mehdi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321575/
https://www.ncbi.nlm.nih.gov/pubmed/30518158
http://dx.doi.org/10.3390/ijms19123881
_version_ 1783385475200843776
author Foroughi, Kobra
Amini, Mohammad
Atashi, Amir
Mahmoodzadeh, Habibollah
Hamann, Ute
Manoochehri, Mehdi
author_facet Foroughi, Kobra
Amini, Mohammad
Atashi, Amir
Mahmoodzadeh, Habibollah
Hamann, Ute
Manoochehri, Mehdi
author_sort Foroughi, Kobra
collection PubMed
description Gastric cancer (GC) is the fifth most common cancer and the third most frequent cause of cancer deaths worldwide. The high death rate associated with GC, and lack of appropriate biomarkers for diagnosis, prognosis, and treatment emphasize the need for identification of novel molecules. Given the emerging roles for long non-coding RNAs (lncRNAs) in cancer development, we studied novel lncRNA candidates involved in gastric carcinogenesis. LncRNA candidate discovery was performed using analyses of available datasets and literature. Validation was done using an internal sample set of GC/normal tissues, and external independent datasets. Network analysis and functional annotation of co-expressed protein coding genes were performed using the weighted gene correlation network analysis (WGCNA) and ingenuity pathway analysis. Two novel lncRNAs, PCAT18 and LINC01133, associated with GC development were identified by analysis of the discovery Gene Expression Omnibus (GEO) datasets. The down-regulation of these genes in GC tissues was successfully validated internally and externally. The results showed a tissue-specific down-regulation of PCAT18 and LINC01133 in gastrointestinal tissues. WGCNA and ingenuity pathway analyses revealed that the genes co-expressed with the two lncRNAs were mostly involved in metabolic pathways and networks of gastrointestinal disease and function. Our findings of a tissue-specific down-regulation of PCAT18 and LINC01133 in gastric and other gastrointestinal cancers imply that these lncRNAs may have a tumor suppressive function in the development of these tumor entities. The two lncRNA biomarkers may contribute to a better understanding of the complex mechanisms of gastric carcinogenesis.
format Online
Article
Text
id pubmed-6321575
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-63215752019-01-07 Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development Foroughi, Kobra Amini, Mohammad Atashi, Amir Mahmoodzadeh, Habibollah Hamann, Ute Manoochehri, Mehdi Int J Mol Sci Article Gastric cancer (GC) is the fifth most common cancer and the third most frequent cause of cancer deaths worldwide. The high death rate associated with GC, and lack of appropriate biomarkers for diagnosis, prognosis, and treatment emphasize the need for identification of novel molecules. Given the emerging roles for long non-coding RNAs (lncRNAs) in cancer development, we studied novel lncRNA candidates involved in gastric carcinogenesis. LncRNA candidate discovery was performed using analyses of available datasets and literature. Validation was done using an internal sample set of GC/normal tissues, and external independent datasets. Network analysis and functional annotation of co-expressed protein coding genes were performed using the weighted gene correlation network analysis (WGCNA) and ingenuity pathway analysis. Two novel lncRNAs, PCAT18 and LINC01133, associated with GC development were identified by analysis of the discovery Gene Expression Omnibus (GEO) datasets. The down-regulation of these genes in GC tissues was successfully validated internally and externally. The results showed a tissue-specific down-regulation of PCAT18 and LINC01133 in gastrointestinal tissues. WGCNA and ingenuity pathway analyses revealed that the genes co-expressed with the two lncRNAs were mostly involved in metabolic pathways and networks of gastrointestinal disease and function. Our findings of a tissue-specific down-regulation of PCAT18 and LINC01133 in gastric and other gastrointestinal cancers imply that these lncRNAs may have a tumor suppressive function in the development of these tumor entities. The two lncRNA biomarkers may contribute to a better understanding of the complex mechanisms of gastric carcinogenesis. MDPI 2018-12-04 /pmc/articles/PMC6321575/ /pubmed/30518158 http://dx.doi.org/10.3390/ijms19123881 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Foroughi, Kobra
Amini, Mohammad
Atashi, Amir
Mahmoodzadeh, Habibollah
Hamann, Ute
Manoochehri, Mehdi
Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development
title Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development
title_full Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development
title_fullStr Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development
title_full_unstemmed Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development
title_short Tissue-Specific Down-Regulation of the Long Non-Coding RNAs PCAT18 and LINC01133 in Gastric Cancer Development
title_sort tissue-specific down-regulation of the long non-coding rnas pcat18 and linc01133 in gastric cancer development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321575/
https://www.ncbi.nlm.nih.gov/pubmed/30518158
http://dx.doi.org/10.3390/ijms19123881
work_keys_str_mv AT foroughikobra tissuespecificdownregulationofthelongnoncodingrnaspcat18andlinc01133ingastriccancerdevelopment
AT aminimohammad tissuespecificdownregulationofthelongnoncodingrnaspcat18andlinc01133ingastriccancerdevelopment
AT atashiamir tissuespecificdownregulationofthelongnoncodingrnaspcat18andlinc01133ingastriccancerdevelopment
AT mahmoodzadehhabibollah tissuespecificdownregulationofthelongnoncodingrnaspcat18andlinc01133ingastriccancerdevelopment
AT hamannute tissuespecificdownregulationofthelongnoncodingrnaspcat18andlinc01133ingastriccancerdevelopment
AT manoochehrimehdi tissuespecificdownregulationofthelongnoncodingrnaspcat18andlinc01133ingastriccancerdevelopment