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Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment
In this methodological paper, lyophilized fibroin-coated alginate microcarriers (LFAMs) proposed as mesenchymal stem cells (MSCs) delivery systems and optimal MSCs seeding conditions for cell adhesion rate and cell arrangement, was defined by a Design of Experiment (DoE) approach. Cells were co-incu...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321597/ https://www.ncbi.nlm.nih.gov/pubmed/30352986 http://dx.doi.org/10.3390/pharmaceutics10040200 |
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author | Perucca Orfei, Carlotta Talò, Giuseppe Viganò, Marco Perteghella, Sara Lugano, Gaia Fabro Fontana, Francesca Ragni, Enrico Colombini, Alessandra De Luca, Paola Moretti, Matteo Torre, Maria Luisa de Girolamo, Laura |
author_facet | Perucca Orfei, Carlotta Talò, Giuseppe Viganò, Marco Perteghella, Sara Lugano, Gaia Fabro Fontana, Francesca Ragni, Enrico Colombini, Alessandra De Luca, Paola Moretti, Matteo Torre, Maria Luisa de Girolamo, Laura |
author_sort | Perucca Orfei, Carlotta |
collection | PubMed |
description | In this methodological paper, lyophilized fibroin-coated alginate microcarriers (LFAMs) proposed as mesenchymal stem cells (MSCs) delivery systems and optimal MSCs seeding conditions for cell adhesion rate and cell arrangement, was defined by a Design of Experiment (DoE) approach. Cells were co-incubated with microcarriers in a bioreactor for different time intervals and conditions: variable stirring speed, dynamic culture intermittent or continuous, and different volumes of cells-LFAMs loaded in the bioreactor. Intermittent dynamic culture resulted as the most determinant parameter; the volume of LFAMs/cells suspension and the speed used for the dynamic culture contributed as well, whereas time was a less influencing parameter. The optimized seeding conditions were: 98 min of incubation time, 12.3 RPM of speed, and 401.5 µL volume of cells-LFAMs suspension cultured with the intermittent dynamic condition. This DoE predicted protocol was then validated on both human Adipose-derived Stem Cells (hASCs) and human Bone Marrow Stem Cells (hBMSCs), revealing a good cell adhesion rate on the surface of the carriers. In conclusion, microcarriers can be used as cell delivery systems at the target site (by injection or arthroscopic technique), to maintain MSCs and their activity at the injured site for regenerative medicine. |
format | Online Article Text |
id | pubmed-6321597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-63215972019-01-11 Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment Perucca Orfei, Carlotta Talò, Giuseppe Viganò, Marco Perteghella, Sara Lugano, Gaia Fabro Fontana, Francesca Ragni, Enrico Colombini, Alessandra De Luca, Paola Moretti, Matteo Torre, Maria Luisa de Girolamo, Laura Pharmaceutics Article In this methodological paper, lyophilized fibroin-coated alginate microcarriers (LFAMs) proposed as mesenchymal stem cells (MSCs) delivery systems and optimal MSCs seeding conditions for cell adhesion rate and cell arrangement, was defined by a Design of Experiment (DoE) approach. Cells were co-incubated with microcarriers in a bioreactor for different time intervals and conditions: variable stirring speed, dynamic culture intermittent or continuous, and different volumes of cells-LFAMs loaded in the bioreactor. Intermittent dynamic culture resulted as the most determinant parameter; the volume of LFAMs/cells suspension and the speed used for the dynamic culture contributed as well, whereas time was a less influencing parameter. The optimized seeding conditions were: 98 min of incubation time, 12.3 RPM of speed, and 401.5 µL volume of cells-LFAMs suspension cultured with the intermittent dynamic condition. This DoE predicted protocol was then validated on both human Adipose-derived Stem Cells (hASCs) and human Bone Marrow Stem Cells (hBMSCs), revealing a good cell adhesion rate on the surface of the carriers. In conclusion, microcarriers can be used as cell delivery systems at the target site (by injection or arthroscopic technique), to maintain MSCs and their activity at the injured site for regenerative medicine. MDPI 2018-10-24 /pmc/articles/PMC6321597/ /pubmed/30352986 http://dx.doi.org/10.3390/pharmaceutics10040200 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Perucca Orfei, Carlotta Talò, Giuseppe Viganò, Marco Perteghella, Sara Lugano, Gaia Fabro Fontana, Francesca Ragni, Enrico Colombini, Alessandra De Luca, Paola Moretti, Matteo Torre, Maria Luisa de Girolamo, Laura Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment |
title | Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment |
title_full | Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment |
title_fullStr | Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment |
title_full_unstemmed | Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment |
title_short | Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment |
title_sort | silk/fibroin microcarriers for mesenchymal stem cell delivery: optimization of cell seeding by the design of experiment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321597/ https://www.ncbi.nlm.nih.gov/pubmed/30352986 http://dx.doi.org/10.3390/pharmaceutics10040200 |
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