Cargando…

Silk/Fibroin Microcarriers for Mesenchymal Stem Cell Delivery: Optimization of Cell Seeding by the Design of Experiment

In this methodological paper, lyophilized fibroin-coated alginate microcarriers (LFAMs) proposed as mesenchymal stem cells (MSCs) delivery systems and optimal MSCs seeding conditions for cell adhesion rate and cell arrangement, was defined by a Design of Experiment (DoE) approach. Cells were co-incu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Perucca Orfei, Carlotta, Talò, Giuseppe, Viganò, Marco, Perteghella, Sara, Lugano, Gaia, Fabro Fontana, Francesca, Ragni, Enrico, Colombini, Alessandra, De Luca, Paola, Moretti, Matteo, Torre, Maria Luisa, de Girolamo, Laura
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321597/ https://www.ncbi.nlm.nih.gov/pubmed/30352986 http://dx.doi.org/10.3390/pharmaceutics10040200

Ejemplares similares

Evaluation of Different Seeding Methods for Cell-Seeded Collagen Matrix-Supported Autologous Chondrocyte Transplantation
por: Viganò, Marco, et al.
Publicado: (2019)

Fabrication of Innovative Silk/Alginate Microcarriers for Mesenchymal Stem Cell Delivery and Tissue Regeneration
por: Perteghella, Sara, et al.
Publicado: (2017)

Identification of miRNA Reference Genes in Extracellular Vesicles from Adipose Derived Mesenchymal Stem Cells for Studying Osteoarthritis
por: Ragni, Enrico, et al.
Publicado: (2019)

Insights into Inflammatory Priming of Adipose-Derived Mesenchymal Stem Cells: Validation of Extracellular Vesicles-Embedded miRNA Reference Genes as A Crucial Step for Donor Selection
por: Ragni, Enrico, et al.
Publicado: (2019)

Autologous Microfragmented Adipose Tissue Reduces the Catabolic and Fibrosis Response in an In Vitro Model of Tendon Cell Inflammation
por: Viganò, Marco, et al.
Publicado: (2019)

Interaction with hyaluronan matrix and miRNA cargo as contributors for in vitro potential of mesenchymal stem cell-derived extracellular vesicles in a model of human osteoarthritic synoviocytes
por: Ragni, Enrico, et al.
Publicado: (2019)

Secreted Factors and EV-miRNAs Orchestrate the Healing Capacity of Adipose Mesenchymal Stem Cells for the Treatment of Knee Osteoarthritis
por: Ragni, Enrico, et al.
Publicado: (2020)

Inflammatory priming enhances mesenchymal stromal cell secretome potential as a clinical product for regenerative medicine approaches through secreted factors and EV-miRNAs: the example of joint disease
por: Ragni, Enrico, et al.
Publicado: (2020)

Cartilage Protective and Immunomodulatory Features of Osteoarthritis Synovial Fluid-Treated Adipose-Derived Mesenchymal Stem Cells Secreted Factors and Extracellular Vesicles-Embedded miRNAs
por: Ragni, Enrico, et al.
Publicado: (2021)

miRNA Reference Genes in Extracellular Vesicles Released from Amniotic Membrane-Derived Mesenchymal Stromal Cells
por: Ragni, Enrico, et al.
Publicado: (2020)

Silk fibroin/gelatin microcarriers as scaffolds for bone tissue engineering
por: Luetchford, Kim A., et al.
Publicado: (2020)

Innovative Visualization and Quantification of Extracellular Vesicles Interaction with and Incorporation in Target Cells in 3D Microenvironments
por: Ragni, Enrico, et al.
Publicado: (2020)

Characterization of Microfragmented Adipose Tissue Architecture, Mesenchymal Stromal Cell Content and Release of Paracrine Mediators
por: Ragni, Enrico, et al.
Publicado: (2022)

miR-103a-3p and miR-22-5p Are Reliable Reference Genes in Extracellular Vesicles From Cartilage, Adipose Tissue, and Bone Marrow Cells
por: Ragni, Enrico, et al.
Publicado: (2021)

Endogenous Controls for the Evaluation of Osteoarthritis-Related miRNAs in Extracellular Vesicles from Bone-Marrow-Derived Mesenchymal Stromal Cells and the Impact of Osteoarthritis Synovial Fluid
por: Ragni, Enrico, et al.
Publicado: (2022)

Adipose-Derived Mesenchymal Stromal Cells Treated with Interleukin 1 Beta Produced Chondro-Protective Vesicles Able to Fast Penetrate in Cartilage
por: Colombini, Alessandra, et al.
Publicado: (2021)

In Vitro Study of Extracellular Vesicles Migration in Cartilage-Derived Osteoarthritis Samples Using Real-Time Quantitative Multimodal Nonlinear Optics Imaging
por: Mortati, Leonardo, et al.
Publicado: (2020)

In Vitro Induction of Tendon-Specific Markers in Tendon Cells, Adipose- and Bone Marrow-Derived Stem Cells is Dependent on TGFβ3, BMP-12 and Ascorbic Acid Stimulation
por: Perucca Orfei, Carlotta, et al.
Publicado: (2019)

A(2A) adenosine receptors are involved in the reparative response of tendon cells to pulsed electromagnetic fields
por: Colombini, Alessandra, et al.
Publicado: (2020)

Amniotic membrane‐mesenchymal stromal cells secreted factors and extracellular vesicle‐miRNAs: Anti‐inflammatory and regenerative features for musculoskeletal tissues
por: Ragni, Enrico, et al.
Publicado: (2021)

Secreted Factors and Extracellular Vesicles Account for the Immunomodulatory and Tissue Regenerative Properties of Bone-Marrow-Derived Mesenchymal Stromal Cells for Osteoarthritis
por: Ragni, Enrico, et al.
Publicado: (2022)

Joint Tissue Protective and Immune-Modulating miRNA Landscape of Mesenchymal Stromal Cell-Derived Extracellular Vesicles under Different Osteoarthritis-Mimicking Conditions
por: Ragni, Enrico, et al.
Publicado: (2022)

Pulsed electromagnetic fields improve the healing process of Achilles tendinopathy: a pilot study in a rat model
por: Perucca Orfei, Carlotta, et al.
Publicado: (2020)

Vitamin D’s Effect on the Proliferation and Inflammation of Human Intervertebral Disc Cells in Relation to the Functional Vitamin D Receptor Gene FokI Polymorphism
por: De Luca, Paola, et al.
Publicado: (2018)

High-Throughput Gene and Protein Analysis Revealed the Response of Disc Cells to Vitamin D, Depending on the VDR FokI Variants
por: Colombini, Alessandra, et al.
Publicado: (2021)

Tissue-Protective and Anti-Inflammatory Landmark of PRP-Treated Mesenchymal Stromal Cells Secretome for Osteoarthritis
por: Ragni, Enrico, et al.
Publicado: (2022)

Molecular Characterization of Secreted Factors and Extracellular Vesicles-Embedded miRNAs from Bone Marrow-Derived Mesenchymal Stromal Cells in Presence of Synovial Fluid from Osteoarthritis Patients
por: Ragni, Enrico, et al.
Publicado: (2022)

Different culture conditions affect the growth of human tendon stem/progenitor cells (TSPCs) within a mixed tendon cells (TCs) population
por: Viganò, M., et al.
Publicado: (2017)

Human Diseased Articular Cartilage Contains a Mesenchymal Stem Cell-Like Population of Chondroprogenitors with Strong Immunomodulatory Responses
por: De Luca, Paola, et al.
Publicado: (2019)

Reliable Reference Genes for Gene Expression Assessment in Tendon-Derived Cells under Inflammatory and Pro-Fibrotic/Healing Stimuli
por: Ragni, Enrico, et al.
Publicado: (2019)

Mesenchymal stem cells as therapeutic target of biophysical stimulation for the treatment of musculoskeletal disorders
por: Viganò, Marco, et al.
Publicado: (2016)

High Levels of Circulating Type II Collagen Degradation Marker (CTx-II) Are Associated with Specific VDR Polymorphisms in Patients with Adult Vertebral Osteochondrosis
por: Cauci, Sabina, et al.
Publicado: (2017)

The Effect of Three Different Suture Anchors for Rotator Cuff Repair on Primary Cultures of Human Bone Marrow Mesenchymal Stem Cells
por: Thiébat, Gabriele, et al.
Publicado: (2018)

Silk Fibroin Bioink for 3D Printing in Tissue Regeneration: Controlled Release of MSC extracellular Vesicles
por: Bari, Elia, et al.
Publicado: (2023)

Silk Fibroin Hydrogels Incorporated with the Antioxidant Extract of Stryphnodendron adstringens Bark
por: de Brito, Vivian P., et al.
Publicado: (2022)

Impacts of Blended Bombyx mori Silk Fibroin and Recombinant Spider Silk Fibroin Hydrogels on Cell Growth
por: Laomeephol, Chavee, et al.
Publicado: (2021)

Treatment of Achilles Tendinopathy with Autologous Adipose-derived Stromal Vascular Fraction: Results of a Randomized Prospective Clinical Trial
por: de Girolamo, Laura, et al.
Publicado: (2016)

Novel silk fibroin nanoparticles incorporated silk fibroin hydrogel for inhibition of cancer stem cells and tumor growth
por: Wu, Puyuan, et al.
Publicado: (2018)

Silk fibroin microgels as a platform for cell microencapsulation
por: Bono, Nina, et al.
Publicado: (2022)

Superior Osteo-Inductive and Osteo-Conductive Properties of Trabecular Titanium vs. PEEK Scaffolds on Human Mesenchymal Stem Cells: A Proof of Concept for the Use of Fusion Cages
por: Ragni, Enrico, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_oblupfr6uuetp14mrdlfc3tgvo