XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC

OBJECTIVE: The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells. METHODS: The expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detected by...

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Autores principales: Xiao, Zhihua, Wang, Yijun, Ding, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321695/
https://www.ncbi.nlm.nih.gov/pubmed/30627419
http://dx.doi.org/10.1186/s13578-018-0269-4
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author Xiao, Zhihua
Wang, Yijun
Ding, Hao
author_facet Xiao, Zhihua
Wang, Yijun
Ding, Hao
author_sort Xiao, Zhihua
collection PubMed
description OBJECTIVE: The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells. METHODS: The expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detected by transwell assay. TargetScan was used to predict potential targets of miR-29a-3p. RESULTS: In this study, we found that the expression of XPD and miR-29a-3p was downregulated in HCC samples and HCC cell lines. XPD suppressed proliferation and migration of HCC cell via regulating miR-29a-3p expression. Target prediction analysis and dual-luciferase reporter assay confirmed Mdm2 and PDGF-B were direct targets of miR-29a-3p, and miR-29a-3p suppressed proliferation and migration of HCC cells via regulating the expression of Mdm2 or PDGF-B. CONCLUSIONS: Our data indicated that XPD suppressed cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC.
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spelling pubmed-63216952019-01-09 XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC Xiao, Zhihua Wang, Yijun Ding, Hao Cell Biosci Research OBJECTIVE: The aim of this study was to investigate the role of XPD in migration and invasion of hepatocellular carcinoma (HCC) cells. METHODS: The expression of XPD and miR-29a-3p was examined by western blot and qRT-PCR, cell proliferation was detected by MTT assay, cell migration was detected by transwell assay. TargetScan was used to predict potential targets of miR-29a-3p. RESULTS: In this study, we found that the expression of XPD and miR-29a-3p was downregulated in HCC samples and HCC cell lines. XPD suppressed proliferation and migration of HCC cell via regulating miR-29a-3p expression. Target prediction analysis and dual-luciferase reporter assay confirmed Mdm2 and PDGF-B were direct targets of miR-29a-3p, and miR-29a-3p suppressed proliferation and migration of HCC cells via regulating the expression of Mdm2 or PDGF-B. CONCLUSIONS: Our data indicated that XPD suppressed cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC. BioMed Central 2019-01-05 /pmc/articles/PMC6321695/ /pubmed/30627419 http://dx.doi.org/10.1186/s13578-018-0269-4 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Xiao, Zhihua
Wang, Yijun
Ding, Hao
XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC
title XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC
title_full XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC
title_fullStr XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC
title_full_unstemmed XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC
title_short XPD suppresses cell proliferation and migration via miR-29a-3p-Mdm2/PDGF-B axis in HCC
title_sort xpd suppresses cell proliferation and migration via mir-29a-3p-mdm2/pdgf-b axis in hcc
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321695/
https://www.ncbi.nlm.nih.gov/pubmed/30627419
http://dx.doi.org/10.1186/s13578-018-0269-4
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AT dinghao xpdsuppressescellproliferationandmigrationviamir29a3pmdm2pdgfbaxisinhcc

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