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De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim

BACKGROUND: Celastrus angulatus Maxim is a kind of crucial and traditional insecticidal plant widely distributed in the mountains of southwest China. Celangulin V is the efficient insecticidal sesquiterpenoid of C. angulatus and widely used in pest control in China, but the low yield and discontinuo...

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Autores principales: Li, Weiguo, Xu, Ranran, Yan, Xiaoguang, Liang, Dongmei, Zhang, Lei, Qin, Xiaoyu, Caiyin, Qinggele, Zhao, Guangrong, Xiao, Wenhai, Hu, Zhaonong, Qiao, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321707/
https://www.ncbi.nlm.nih.gov/pubmed/30611193
http://dx.doi.org/10.1186/s12864-018-5397-z
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author Li, Weiguo
Xu, Ranran
Yan, Xiaoguang
Liang, Dongmei
Zhang, Lei
Qin, Xiaoyu
Caiyin, Qinggele
Zhao, Guangrong
Xiao, Wenhai
Hu, Zhaonong
Qiao, Jianjun
author_facet Li, Weiguo
Xu, Ranran
Yan, Xiaoguang
Liang, Dongmei
Zhang, Lei
Qin, Xiaoyu
Caiyin, Qinggele
Zhao, Guangrong
Xiao, Wenhai
Hu, Zhaonong
Qiao, Jianjun
author_sort Li, Weiguo
collection PubMed
description BACKGROUND: Celastrus angulatus Maxim is a kind of crucial and traditional insecticidal plant widely distributed in the mountains of southwest China. Celangulin V is the efficient insecticidal sesquiterpenoid of C. angulatus and widely used in pest control in China, but the low yield and discontinuous supply impeded its further popularization and application. Fortunately, the development of synthetic biology provided an opportunity for sustainable supply of Celangulin V, for which understanding its biosynthetic pathway is indispensable. RESULTS: In this study, six cDNA libraries were prepared from leaf and root of C. angulatus before global transcriptome analyses using the BGISEQ-500 platform. A total of 104,950 unigenes were finally obtained with an average length of 1200 bp in six transcriptome databases of C. angulatus, in which 51,817 unigenes classified into 25 KOG classifications, 39,866 unigenes categorized into 55 GO functional groups, and 48,810 unigenes assigned to 135 KEGG pathways, 145 of which were putative biosynthetic genes of sesquiterpenoid and triterpenoid. 16 unigenes were speculated to be related to Celangulin V biosynthesis. De novo assembled sequences were verified by Quantitative Real-Time PCR (qRT-PCR) analysis. CONCLUSIONS: This study is the first report on transcriptome analysis of C. angulatus, and 16 unigenes probably involved in the biosynthesis of Celangulin V were finally collected. The transcriptome data will make great contributions to research for this specific insecticidal plant and the further gene mining for biosynthesis of Celangulin V and other sesquiterpene polyol esters. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5397-z) contains supplementary material, which is available to authorized users.
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spelling pubmed-63217072019-01-09 De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim Li, Weiguo Xu, Ranran Yan, Xiaoguang Liang, Dongmei Zhang, Lei Qin, Xiaoyu Caiyin, Qinggele Zhao, Guangrong Xiao, Wenhai Hu, Zhaonong Qiao, Jianjun BMC Genomics Research Article BACKGROUND: Celastrus angulatus Maxim is a kind of crucial and traditional insecticidal plant widely distributed in the mountains of southwest China. Celangulin V is the efficient insecticidal sesquiterpenoid of C. angulatus and widely used in pest control in China, but the low yield and discontinuous supply impeded its further popularization and application. Fortunately, the development of synthetic biology provided an opportunity for sustainable supply of Celangulin V, for which understanding its biosynthetic pathway is indispensable. RESULTS: In this study, six cDNA libraries were prepared from leaf and root of C. angulatus before global transcriptome analyses using the BGISEQ-500 platform. A total of 104,950 unigenes were finally obtained with an average length of 1200 bp in six transcriptome databases of C. angulatus, in which 51,817 unigenes classified into 25 KOG classifications, 39,866 unigenes categorized into 55 GO functional groups, and 48,810 unigenes assigned to 135 KEGG pathways, 145 of which were putative biosynthetic genes of sesquiterpenoid and triterpenoid. 16 unigenes were speculated to be related to Celangulin V biosynthesis. De novo assembled sequences were verified by Quantitative Real-Time PCR (qRT-PCR) analysis. CONCLUSIONS: This study is the first report on transcriptome analysis of C. angulatus, and 16 unigenes probably involved in the biosynthesis of Celangulin V were finally collected. The transcriptome data will make great contributions to research for this specific insecticidal plant and the further gene mining for biosynthesis of Celangulin V and other sesquiterpene polyol esters. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12864-018-5397-z) contains supplementary material, which is available to authorized users. BioMed Central 2019-01-05 /pmc/articles/PMC6321707/ /pubmed/30611193 http://dx.doi.org/10.1186/s12864-018-5397-z Text en © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Li, Weiguo
Xu, Ranran
Yan, Xiaoguang
Liang, Dongmei
Zhang, Lei
Qin, Xiaoyu
Caiyin, Qinggele
Zhao, Guangrong
Xiao, Wenhai
Hu, Zhaonong
Qiao, Jianjun
De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim
title De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim
title_full De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim
title_fullStr De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim
title_full_unstemmed De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim
title_short De novo leaf and root transcriptome analysis to explore biosynthetic pathway of Celangulin V in Celastrus angulatus maxim
title_sort de novo leaf and root transcriptome analysis to explore biosynthetic pathway of celangulin v in celastrus angulatus maxim
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321707/
https://www.ncbi.nlm.nih.gov/pubmed/30611193
http://dx.doi.org/10.1186/s12864-018-5397-z
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