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Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons

BACKGROUND: Transcranial ultrasonic stimulation is a novel noninvasive tool for neuromodulation, and has high spatial resolution and deep penetration. Although it can increase excitation of neurons, its effects on neuron are poorly understood. This study was to evaluate effect of ultrasonic stimulat...

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Autores principales: Cui, Kun, Zhang, Shuai, Sun, Jinyao, Zhang, Xueying, Ding, Chong, Xu, Guizhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321733/
https://www.ncbi.nlm.nih.gov/pubmed/30611209
http://dx.doi.org/10.1186/s12868-018-0485-1
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author Cui, Kun
Zhang, Shuai
Sun, Jinyao
Zhang, Xueying
Ding, Chong
Xu, Guizhi
author_facet Cui, Kun
Zhang, Shuai
Sun, Jinyao
Zhang, Xueying
Ding, Chong
Xu, Guizhi
author_sort Cui, Kun
collection PubMed
description BACKGROUND: Transcranial ultrasonic stimulation is a novel noninvasive tool for neuromodulation, and has high spatial resolution and deep penetration. Although it can increase excitation of neurons, its effects on neuron are poorly understood. This study was to evaluate effect of ultrasonic stimulation (US) on neurons in vitro. In this paper, the effect of US on the excitability and voltage-dependent [Formula: see text] currents of CA1 pyramidal neurons in the rat hippocampus was studied using patch clamp. RESULTS: Our results suggest that US increased the spontaneous firing rate and inhibited transient outward potassium current ([Formula: see text] ) and delayed rectifier potassium current ([Formula: see text] . Furthermore, US altered the activation of [Formula: see text] channels, inactivation and recovery properties of [Formula: see text] channels. After US, the [Formula: see text] activation curves significantly moved to the negative voltage direction and increased its slope factor. Moreover, the data showed that US moved the inactivation curve of [Formula: see text] to the negative voltage and increased the slope factor. Besides, US delayed the recovery of [Formula: see text] channel. CONCLUSIONS: Our data indicate that US can increase excitation of neurons by inhibiting potassium currents. Different US decreased the voltage sensitivity of [Formula: see text] activation differentially. Moreover, the more time is needed for US to make the [Formula: see text] channels open again after inactivating. US may play a physiological role by inhibiting voltage-dependent potassium currents in neuromodulation. Our research can provide a theoretical basis for the future clinical application of ultrasound in neuromodulation.
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spelling pubmed-63217332019-01-09 Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons Cui, Kun Zhang, Shuai Sun, Jinyao Zhang, Xueying Ding, Chong Xu, Guizhi BMC Neurosci Research Article BACKGROUND: Transcranial ultrasonic stimulation is a novel noninvasive tool for neuromodulation, and has high spatial resolution and deep penetration. Although it can increase excitation of neurons, its effects on neuron are poorly understood. This study was to evaluate effect of ultrasonic stimulation (US) on neurons in vitro. In this paper, the effect of US on the excitability and voltage-dependent [Formula: see text] currents of CA1 pyramidal neurons in the rat hippocampus was studied using patch clamp. RESULTS: Our results suggest that US increased the spontaneous firing rate and inhibited transient outward potassium current ([Formula: see text] ) and delayed rectifier potassium current ([Formula: see text] . Furthermore, US altered the activation of [Formula: see text] channels, inactivation and recovery properties of [Formula: see text] channels. After US, the [Formula: see text] activation curves significantly moved to the negative voltage direction and increased its slope factor. Moreover, the data showed that US moved the inactivation curve of [Formula: see text] to the negative voltage and increased the slope factor. Besides, US delayed the recovery of [Formula: see text] channel. CONCLUSIONS: Our data indicate that US can increase excitation of neurons by inhibiting potassium currents. Different US decreased the voltage sensitivity of [Formula: see text] activation differentially. Moreover, the more time is needed for US to make the [Formula: see text] channels open again after inactivating. US may play a physiological role by inhibiting voltage-dependent potassium currents in neuromodulation. Our research can provide a theoretical basis for the future clinical application of ultrasound in neuromodulation. BioMed Central 2019-01-05 /pmc/articles/PMC6321733/ /pubmed/30611209 http://dx.doi.org/10.1186/s12868-018-0485-1 Text en © The Author(s) 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Cui, Kun
Zhang, Shuai
Sun, Jinyao
Zhang, Xueying
Ding, Chong
Xu, Guizhi
Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons
title Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons
title_full Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons
title_fullStr Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons
title_full_unstemmed Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons
title_short Inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal CA1 neurons
title_sort inhibitory effect of ultrasonic stimulation on the voltage-dependent potassium currents in rat hippocampal ca1 neurons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321733/
https://www.ncbi.nlm.nih.gov/pubmed/30611209
http://dx.doi.org/10.1186/s12868-018-0485-1
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