Cargando…

The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats

Radioactive dermatitis is caused by the exposure of skin and mucous membranes to radiation fields. The pathogenesis of radioactive dermatitis is complex and difficult to cure. Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) may serve as a promising candidate for the therapy of cutaneous wou...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, JiaYang, Zhang, YunFeng, Song, XianJi, Zhu, Jiajing, Zhu, QingSan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322144/
https://www.ncbi.nlm.nih.gov/pubmed/30350716
http://dx.doi.org/10.1177/0963689718807410
_version_ 1783385561816367104
author Sun, JiaYang
Zhang, YunFeng
Song, XianJi
Zhu, Jiajing
Zhu, QingSan
author_facet Sun, JiaYang
Zhang, YunFeng
Song, XianJi
Zhu, Jiajing
Zhu, QingSan
author_sort Sun, JiaYang
collection PubMed
description Radioactive dermatitis is caused by the exposure of skin and mucous membranes to radiation fields. The pathogenesis of radioactive dermatitis is complex and difficult to cure. Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) may serve as a promising candidate for the therapy of cutaneous wounds. The aim of this study was to investigate whether a WJ-MSC-derived conditioned medium (MSC-CM) could be used to treat radiation-induced skin wounds in rats using a radiation-induced cutaneous injury model. The present study was designed to examine MSC-CM therapy in the recovery of radiation-induced skin wounds in vitro and in vivo. Firstly, we prepared the MSC-CM and tested the effects of the MSC-CM on human umbilical vein endothelial cell proliferation in vitro. After that, we used a β-ray beam to make skin wounds in rats and tested the effects of MSC-CM on cutaneous wound healing in vivo. Our results indicated that MSC-CM secreted factors that promoted HUVEC proliferation, regeneration of sebaceous glands, and angiogenesis. Importantly, MSC-CM promoted wound healing in excess of the positive control (epidermal growth factor), with no, or smaller, scar formation. In conclusion, MSC-CM significantly accelerated wound closure and enhanced the wound healing quality. MSC-CM has a beneficial therapeutic effect on radiation-induced cutaneous injury skin in rats and in this way MSC-CM may serve as a basis of a novel cell-free therapeutic approach for radiation dermatitis.
format Online
Article
Text
id pubmed-6322144
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher SAGE Publications
record_format MEDLINE/PubMed
spelling pubmed-63221442019-01-14 The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats Sun, JiaYang Zhang, YunFeng Song, XianJi Zhu, Jiajing Zhu, QingSan Cell Transplant Original Articles Radioactive dermatitis is caused by the exposure of skin and mucous membranes to radiation fields. The pathogenesis of radioactive dermatitis is complex and difficult to cure. Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs) may serve as a promising candidate for the therapy of cutaneous wounds. The aim of this study was to investigate whether a WJ-MSC-derived conditioned medium (MSC-CM) could be used to treat radiation-induced skin wounds in rats using a radiation-induced cutaneous injury model. The present study was designed to examine MSC-CM therapy in the recovery of radiation-induced skin wounds in vitro and in vivo. Firstly, we prepared the MSC-CM and tested the effects of the MSC-CM on human umbilical vein endothelial cell proliferation in vitro. After that, we used a β-ray beam to make skin wounds in rats and tested the effects of MSC-CM on cutaneous wound healing in vivo. Our results indicated that MSC-CM secreted factors that promoted HUVEC proliferation, regeneration of sebaceous glands, and angiogenesis. Importantly, MSC-CM promoted wound healing in excess of the positive control (epidermal growth factor), with no, or smaller, scar formation. In conclusion, MSC-CM significantly accelerated wound closure and enhanced the wound healing quality. MSC-CM has a beneficial therapeutic effect on radiation-induced cutaneous injury skin in rats and in this way MSC-CM may serve as a basis of a novel cell-free therapeutic approach for radiation dermatitis. SAGE Publications 2018-10-23 2019-01 /pmc/articles/PMC6322144/ /pubmed/30350716 http://dx.doi.org/10.1177/0963689718807410 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Sun, JiaYang
Zhang, YunFeng
Song, XianJi
Zhu, Jiajing
Zhu, QingSan
The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats
title The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats
title_full The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats
title_fullStr The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats
title_full_unstemmed The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats
title_short The Healing Effects of Conditioned Medium Derived from Mesenchymal Stem Cells on Radiation-Induced Skin Wounds in Rats
title_sort healing effects of conditioned medium derived from mesenchymal stem cells on radiation-induced skin wounds in rats
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322144/
https://www.ncbi.nlm.nih.gov/pubmed/30350716
http://dx.doi.org/10.1177/0963689718807410
work_keys_str_mv AT sunjiayang thehealingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT zhangyunfeng thehealingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT songxianji thehealingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT zhujiajing thehealingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT zhuqingsan thehealingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT sunjiayang healingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT zhangyunfeng healingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT songxianji healingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT zhujiajing healingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats
AT zhuqingsan healingeffectsofconditionedmediumderivedfrommesenchymalstemcellsonradiationinducedskinwoundsinrats