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Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones
Pheromone (PHB)-receptor (RCB) interaction in the mating pheromone response pathway of Lentinula edodes was investigated using synthetic PHBs. Functionality of the C-terminally carboxymethylated synthetic PHBs was demonstrated by concentration-dependent induction of a mating-related gene (znf2) expr...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322375/ https://www.ncbi.nlm.nih.gov/pubmed/30637149 http://dx.doi.org/10.1080/12298093.2018.1541518 |
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author | Ha, Byeongsuk Kim, Sinil Kim, Minseek Ro, Hyeon-Su |
author_facet | Ha, Byeongsuk Kim, Sinil Kim, Minseek Ro, Hyeon-Su |
author_sort | Ha, Byeongsuk |
collection | PubMed |
description | Pheromone (PHB)-receptor (RCB) interaction in the mating pheromone response pathway of Lentinula edodes was investigated using synthetic PHBs. Functionality of the C-terminally carboxymethylated synthetic PHBs was demonstrated by concentration-dependent induction of a mating-related gene (znf2) expression and by pseudoclamp formation in a monokaryotic strain S1-11 of L. edodes. Treatment with synthetic PHBs activated the expression of homeodomain genes (HDs) residing in the A mating type locus, and of A-regulated genes, including znf2, clp1, and priA, as well as genes in the B mating type locus, including pheromone (phb) and receptor (rcb) genes. The synthetic PHBs failed to discriminate self from non-self RCBs. PHBs of the B4 mating type (B4 PHBs) were able to activate the mating pheromone response pathway in both monokaryotic S1-11 and S1-13 strains, whose B mating types were B4 (self) and B12 (non-self), respectively. The same was true for B12 PHBs in the B4 (non-self) and B12 (self) mating types. The synthetic PHBs also promoted the mating of two monokaryotic strains carrying B4-common incompatible mating types (A5B4 × A1B4). However, the dikaryon generated by this process exhibited abnormally high content of hyphal branching and frequent clamp connections and, more importantly, was found to be genetically unstable due to overexpression of mating-related genes such as clp1. Although synthetic PHBs were unable to discriminate self from non-self RCBs, they showed a higher affinity for non-self RCBs, through which the mating pheromone response pathway in non-self cells may be preferentially activated. |
format | Online Article Text |
id | pubmed-6322375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-63223752019-01-11 Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones Ha, Byeongsuk Kim, Sinil Kim, Minseek Ro, Hyeon-Su Mycobiology Research Articles Pheromone (PHB)-receptor (RCB) interaction in the mating pheromone response pathway of Lentinula edodes was investigated using synthetic PHBs. Functionality of the C-terminally carboxymethylated synthetic PHBs was demonstrated by concentration-dependent induction of a mating-related gene (znf2) expression and by pseudoclamp formation in a monokaryotic strain S1-11 of L. edodes. Treatment with synthetic PHBs activated the expression of homeodomain genes (HDs) residing in the A mating type locus, and of A-regulated genes, including znf2, clp1, and priA, as well as genes in the B mating type locus, including pheromone (phb) and receptor (rcb) genes. The synthetic PHBs failed to discriminate self from non-self RCBs. PHBs of the B4 mating type (B4 PHBs) were able to activate the mating pheromone response pathway in both monokaryotic S1-11 and S1-13 strains, whose B mating types were B4 (self) and B12 (non-self), respectively. The same was true for B12 PHBs in the B4 (non-self) and B12 (self) mating types. The synthetic PHBs also promoted the mating of two monokaryotic strains carrying B4-common incompatible mating types (A5B4 × A1B4). However, the dikaryon generated by this process exhibited abnormally high content of hyphal branching and frequent clamp connections and, more importantly, was found to be genetically unstable due to overexpression of mating-related genes such as clp1. Although synthetic PHBs were unable to discriminate self from non-self RCBs, they showed a higher affinity for non-self RCBs, through which the mating pheromone response pathway in non-self cells may be preferentially activated. Taylor & Francis 2018-12-21 /pmc/articles/PMC6322375/ /pubmed/30637149 http://dx.doi.org/10.1080/12298093.2018.1541518 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of the Korean Society of Mycology. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Ha, Byeongsuk Kim, Sinil Kim, Minseek Ro, Hyeon-Su Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones |
title | Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones |
title_full | Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones |
title_fullStr | Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones |
title_full_unstemmed | Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones |
title_short | Activation of the Mating Pheromone Response Pathway of Lentinula edodes by Synthetic Pheromones |
title_sort | activation of the mating pheromone response pathway of lentinula edodes by synthetic pheromones |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322375/ https://www.ncbi.nlm.nih.gov/pubmed/30637149 http://dx.doi.org/10.1080/12298093.2018.1541518 |
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