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Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time
This study aimed to establish a protocol for in vitro embryo production using epididymal sperm (EP). Samples were obtained from ejaculated sperm (EJ) and the epididymis of 7 Gir bulls. First, the effect of heparin (+) on the viability, longevity (Experiment 1) and fertilization rates (Experiment 2)...
Autores principales: | Cunha, Andrielle T. M., Carvalho, José O., Guimarães, Ana L. S., Leme, Ligiane O., Caixeta, Felippe M., Viana, João H. M., Dode, Margot A. N. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322719/ https://www.ncbi.nlm.nih.gov/pubmed/30615639 http://dx.doi.org/10.1371/journal.pone.0209692 |
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